Koyunlarda Campylobacter Taşıyıcılığı ve Serum Antikor Düzeyleri Arasındaki ilişki

Year 2000, Volume: 11 Issue: 1-2, 31 - 46, 01.12.2000

Nahit Yazıcıoğlu

Abstract

Bu çalışmada . mezbahada kesilen koyunların safra keselerinde Campylobacter türlerinin
ve serumlarında anti-Campylobacter antikarlarının sıklıgı incelendi. Dokuz farklı orijinden gelen
ı80 koyunun % 52.8'inin safra keselerinden Campylobacter'ler izole edildi. İzolasyon oranları,
7-8 aylık koyunlarda% 33.3, 2-3 yaşlı koyunlarda% 59.3 olarak belirlendi. izole edilen 95
Campylobacter suşunun, 66'sı C.jetus subsp.jetus, 22'si C.jejuni ve Tsi C. coli olarak identiliye
edildi.
Komplement fikzasyon testinde, 180 koyunun% 36.7'sı 1:4'ten 1:32'ye kadar degişen titrelerde
C. jetus subsp. fetus antijenine karşı pozitif reaksiyon verdi. Kuzuların % 22.2'sinde,
erişkin koyunların% 41.5'inde serapozitiflik saptandı. Kuzu serumlarının çogu 1:4 titrede pozitif
bulunurken, pozitif erişkin koyun serumları 1 :4- 1 :32 titreler arasında nisbeten dengeli dagılım
gösterdi. C.jetus subsp. fetus izole edilen koyunların çogu (% 84.4) aynı zamanda serapozitif
bulunurken, C. jejuni/ co li izole edilen koyunların sadece % 17 .2'si seropozitif bulundu. Bu
durum iki Campylobacter grubu arasında düşük düzeyde antijenik ilişki oldugunu gösterdi.
Bu bulgular, Campylobacter'lerin özellikle erişkin koyunların safra keselerinde yaygın oldugunu
ve asemptomatik Campylobacter taşıyıcılıgının önemli düzeyde antikor üretimine neden
olabilecegini gösterdi. Ayrıca, halen kullanılan pozitiflik kriterleri ile komplement fikzasyon
testinin, asemptomatik taşıyıcıları ve abort yapan koyunları ayıramayacagı belirlendi.
Sonuç olarak, abortif Campylobacter infeksiyonlarının teşhisi için kullanılan komplement
fikzasyon testinin pozitiflik kriterleri ve antijen spektrumunun tekrar gözden geçirilmesi gerektigi
kanısına varıldı.

Keywords

Nahit YAZICIOĞLU, 2000, Etlik Veteriner Mikrobiyoloji Dergisi, Campylobacter, Koyun, safra kesesi, izolasyon, seroloji.

The Relationship Between Campylobacter Colonization And Serum Antibody Levels in Sheep

Abstract

In this study, the prevaience of Campylobacter spp. in the gallbladder and anti-Campylobacter
antibodies in the sera of slaughtered sheep were investigated. Campylobacters were isolated
from 52.8 % of gallbladders of ı8o sheep from nine different origins. Isolation rates were
determined as 33.3 % in 7-8 months old sheep. and 59.3 % in 2-3 years old ones. Out of 95
Campylobacter strains isolated, 66 were identified as C. jetus subsp. jetus. 22 as C. jejuni and
7 as C. coli. In complement fıxatlon test, 36.7% of sera from ı80 sheep gave positlve reactlons against
C.jetus subsp.jetus antigen. Seropositlvity was detected in 22.2% of lambs, and 4ı.5% of older
sheep. The most of the sera from lambs were found positive at ı:4 tlter, whereas the positive
sera from mature sheep showed a relatlvely homogenous distribution with titers from 1:4
to 1:32. The most (84.8 %) of sheep from which C.jetus subsp.jetus was isolated were also found
as seropositlve. Only 17.2% of sheep from which C.jejuni/coli was isolated, were found as
seropositlve, indicating the low level of antlgenic relationship between these two groups of
campylobacters.
These findings showed that campylobacters were common in the gallbladder of sheep, particularly
in older ones and asymptomatic carriage of campylobacters could cause antibody production
in a considerable level. It was also determined that complement fıxation test with currently
used criteria was inadequate to differentlate the asymptomatically infected and aborted
sheep.
It was concluded that the diagnostic criteria and antigen spectrum of complement fıxation
test to detect abortlve Campylobacter infection should be reevaluated.

Keywords

Sheep, Campylobacter, gall bladder, isolatlon, serology.

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