The Comparision of the ELISA and The Gamma-İnterferon Assay for the Diagnosis of Bovine Tuberculosis

Year 2002, Volume: 13 Issue: 2, 7 - 15, 01.12.2002

Erhan Akçay Müjgan İzgür

Abstract

In the present study, the blood samples from 536 animal s, checked w ith tu bereulin each year and the samples from 2'10 animals
which have never been checked with tuberculin have been tested with ELISA. As a result 582 of them (78%) have resulred
in negative and 164 of them (22%) have resulred in positive. In the group of checked animals there were 57 ( 11 %) positive
and 479 (89%) negative samples and in the group of unchecked animals there were 107 (51%) pasit/ve and 103 (49%)
negative samples. 75 samples from checked animals, resulred in negative in ELISA and 75 samples ,chosen from the unchecked
animals ,resulted in positive have been tesred with y-IFN assay. 42 of the unchecked samples (56%) have given positive and
33 (44%) ofthem negative, 61 of the checked samples (8'1.3%) have given negative and 14 of them (18.7%) havegiven
positive results.
The difference between the positive results (p<0.001) is important, between the negative results (p>0.05) is unimportant and
the difference between the evafuarian of the results of the tests (p

Sığır Tüberkülozunun Teşhisinde Kullanılan Gamma İnterferon Testi ile Elısa'nın Karşılaştırmalı Değerlendirmesi

Abstract

Bu çalışmada, tüberkülin testi ile periyodik kontrolleri yapılan 536 adet ve test edilmeyen kontrolsüz 210 adet hayvandan
kan örnekleri toplanarak önce ELISA'da değerlendirilmiştir. ELISA'da örneklerin 582'si (%78) negatif 164'ü (%22)
pozitifbulunmuştur. Kontrollü hayvanlarda 57 (%11) pozitif, 479 (%89) negatif kontrolsüz hayvanlarda '107 (%51) pozitif,
103 (%49) negatif sonuç saptanmıştır. Kontrollü hayvanlarda negatif bulunan 75 adet örnek ile kontrolsüz
hayvanlarda pozitif bulunan 75 adet örnek y-IFN testinde kullanılmıştır. y-IFN testinde kontrolsüz hayvanlardan seçilen
örneklerin 42'si (%56) pozitif 33'ü (%44) negatif, kontrollü hayvanlardan seçilen örneklerin 61'1 (%8'1.3) negatif ,14'ü
(%18. 7) pozitifsonuç vermiştir.
Her iki testten elde edilen sonuçların khi kare (x2) yöntemi ile istatistiksel değerlendirilmelerinde, pozitif sonuçlar arasındaki
farkın (p<0.001) önemli, negatif sonuçlar arasındaki farkın (p>0.05) önemsiz ve test sonuçlarınm ortak
değerlendirilmelerinde ise testler arasındaki farkın (p<0.01) önemli olduğu görülmüştür.

Keywords

Etlik Veteriner Mikrobiyoloji Dergisi, Sığır, Tüberküloz, Elisa, Gamma İnterforen, 2002

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