Türkiye’de Gökkuşağı Alabalığı Çiftliklerinden İzole Edilen Listonella Anguillarum Suşlarının Fenotipik Özel likleri

Year 2012, Volume: 23 Issue: 2, 44 - 48, 31.12.2012

Seçil Metin

Abstract

Bu çalışmada Türkiye’de İç Anadolu ve Akdeniz bölgesinde bulunan 5 gökkuşağı alabalığı çiftliklerinde meydana
gelen salgınlardan izole edilen 9 Listonella anguillarum izolatının fenotipik özellikleri belirlenmiştir. Enfekte
gökkuşağı alabalıklarında letarji, deri renginde koyulaşma, bilateral ekzoftalmus, pullarda dökülme, vücut yüzeyinde
furunkuller, deri, ağız, göz, yüzgeç ve anüste hemorajiler görülmüştür. İç bakıda asites, karaciğer, yağ doku ve kaslarda
hemoraji, karaciğer ve böbrekte solgunluk, dalakta genişleme, mide ve bağırsakta sarı renkli kanlı sıvı birikimi tespit
edilmiştir. Bakteriyolojik incelemeler için karaciğer ve böbrekten alınan örnekler, %1 NaCl ilave edilmiş Triptik Soy
Agara ekilmiş ve 25°C de 48 saat süre ile inkübe edilmiştir. L.anguillarum izolatları (n=9) geleneksel bakteriyolojik
yöntemler ve API 20 E kullanılarak identifiye edilmiştir. İzolatların fenotipik özelliklerinin homojen olduğutespit edilmiştir.
Yapılan antibiyogram testinde tüm izolatların trimethoprim, tetrasiklin, oksitetrasiklin, enoksasin’e duyarlı ve
flukloksasillin, sülfamethakzol, amoksisillin ve ampisilline karşı dirençli oldukları belirlenmiştir.

Keywords

Etlik Veteriner Mikrobiyoloji Dergisi, Gökkuşağı Alabalığı, İzole, Listonella Anguillarum, Fenotipik, Şeçil METİN, 2012

Phenotypic Characteristics of Listonella Anguillarum Strains Isolated From Rainbow Trout (Oncorhyn- Chus mykiss, Walbaum) Farms in Turkey

Abstract

In the present study, nine isolates of Listonella anguillarum were characterized isolated from epizootics at
five rainbow trout fresh water farms in Middle and Mediterranean region of Turkey. Infected rainbow trout exhibited
lethargy, dark skin, bilateral exophthalmos, spilling of scales, furuncles on the sides of the body, haemorrhage in the
skin, mouth, eyes, fins and anus. Necropsy findings included haemorrhage in the liver, adipose tissue and muscles,
pale kidney and liver, enlarged spleen, ascites in the body cavity, yellowish-bloody fluid in the stomach and intestine.
Samples for bacteriological examinations were collected from the kidney and liver by using sterile swabs; these samples
were streaked onto trypticase soy agar supplemented with 1% NaCl plates, and incubated at 25°C for 48 h. Nine bacterial
isolates were obtained from sick fish. L.anguillarum isolates was identified by conventional methods and API 20
E. Phenotypic characteristics of the isolates were found as homogenous. All of L.anguillarum isolates were sensitive
to trimethoprim, tetracycline, oxytetracycline, enoxacin and resistance to flucloxacillin, sulfamethoxazole, amoxicillin
and ampicillin.

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