Determination of Neospora caninum in Cattle Fetuses from the Central Black Sea Region Using PCR

Yıl 2024, Cilt: 35 Sayı: 2, 142 - 147, 03.01.2025

Rahşan Koç Akpınar , Selma Kaya , Coşkun Aydın , Şakir Önder Türlek , Sema Nur Çelik

https://doi.org/10.35864/evmd.1557406

Öz

Neosporosis is an infectious disease caused by the Neospora caninum, which leads to abortions in cattle. It causes significant economic losses in both global livestock farming and has recently become one of the leading causes of abortions. This study aims to determine the prevalence of Neospora infection in aborted fetuses from 136 cattle in enterprises where large-scale cattle farming is intensively practiced in the Central Black Sea Region (Amasya, Samsun, and Tokat provinces). For the diagnosis of N. caninum, DNA isolation was performed aborted fetuses, and these samples were subsequently analyzed using the PCR test method. According to the results obtained, N. caninum was found in 3 out of 136 aborted fetuses. In conclusion, this study conducted on cattle in the Central Black Sea Region detected the presence of Neospora caninum in aborted fetal samples at a rate of 2.2%. These findings indicate that N. caninum should not be overlooked in future studies involving aborted fetal samples. Conducting comprehensive research on the definitive hosts of the parasite will play a crucial role in controlling neosporosis and contribute to the development of effective strategies to prevent the spread of the disease.

Anahtar Kelimeler

Cattle, Central Black Sea, Fetus, Neosporosis, PCR

Etik Beyan

Ethical Statement: This study was conducted with permission from the Local Ethics Committee for Animal Experiments of the Samsun Veterinary Control Institute, under the letter dated 07.07.2022 with reference number 19572899/031-65 (Decision no: 2022/5).

Orta Karadeniz Bölgesindeki Sığır Atık Fetüslerinde Neospora Canınum’un Prevelansının PCR ile Taranması

Öz

Neosporozis, Neospora caninum’un neden olduğu, sığırlarda aborta neden olan enfeksiyöz bir hastalıktır. Hastalık, büyükbaş hayvan yetiştiriciliğinde önemli ekonomik kayıplara neden olmakta ve son yıllarda abortların başlıca nedenleri arasında yer almaktadır. Bu çalışma, Orta Karadeniz Bölgesinde (Amasya, Samsun ve Tokat illeri) büyükbaş hayvancılığın yoğun olarak yapıldığı işletmelerde abort yapan 136 sığıra ait atık fetüslerde Neospora sp. varlığını ve prevalansını belirlemeyi hedeflemektedir. Neospora caninum'un teşhisi için atık fetüslerden DNA izolasyonu yapılmış ve ardından bu örnekler PCR test yöntemi ile analiz edilmiştir. Elde edilen sonuçlar doğrultusunda, 136 sığıra ait atık fetüslerden 3’ünde N. caninum’a rastlanmıştır. Sonuç olarak, Orta Karadeniz Bölgesindeki sığırlarda gerçekleştirilen bu çalışmada aborte fetüs numunelerinde N.caninum'un varlığı %2.2 oranında tespit edilmiştir. Bu bulgular, aborte fetüs numuneleri ile yapılacak gelecekteki çalışmalarda N. caninum'un göz ardı edilmemesi gerektiğini göstermektedir. Parazitin son konaklarıyla ilgili kapsamlı araştırmaların yürütülmesi, neosporosisin kontrolünde önemli bir rol oynayacak ve hastalığın yayılmasını engellemeye yönelik etkili stratejiler geliştirilmesine katkı sağlayacaktır.

Anahtar Kelimeler

Fetüs, Neosporosis, Orta Karadeniz, PCR, Sığır

Etik Beyan

Ethical Statement: This study was conducted with permission from the Local Ethics Committee for Animal Experiments of the Samsun Veterinary Control Institute, under the letter dated 07.07.2022 with reference number 19572899/031-65 (Decision no: 2022/5).

Kaynakça

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