Simultaneous Determination of Spiramycin and Metronidazole in Coated Tablets by Derivative and Wavelet Transforms of UV Spectra and Ratio Spectra

Year 2022, Volume: 47 Issue: 3, 301 - 316, 19.10.2022

Doan Thanh Xuan Vu Dang Hoang

Abstract

Signal transformation (derivative and wavelet) was applied to
UV spectra and ratio spectra to directly quantify spiramycin and
metronidazole in binary mixtures. Linear calibration graphs were
examined for either drug in the concentration range of 6.25 – 25
mg/L with R2 > 0.990. First derivative-transformed (i.e., using
Savitzky-Golay filter) and wavelet-transformed (i.e., using families
such as sym6, haar, db5, bior2.4, rbio2.4, meyr with scaling factor =
256) UV spectrophotometric methods were statistically comparable to
the RP-HPLC reference method (p > 0.05) with regard to accuracy
and precision when assaying spiramycin and metronidazole in their
coated tablets. These analytical methods used only green solvent, and
proved to be time-saving and cost-effective.

Keywords

UV spectrophotometry, derivative transform, wavelet transform, spiramycin, metronidazole, coated tablets, RP-HPLC

References

• Abdel-Kader, D. A., & Hashem, E. Y. (2021). Spectrophotometric determination of Metronidazole antibacterial drug via oxidation with alkaline potassium permanganate. Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy, 259, 119858. doi:https://doi.org/10.1016/j.saa.2021.119858