AN INVESTIGATION OF THE ACE INHIBITORY ACTIVITY, ANTIOXIDANT CAPACITY, AND PHYTOCHEMICAL CONSTITUENTS OF POLAR AND NON-POLAR EXTRACTS OF ZIZIPHUS JUJUBA FRUIT: STATISTICAL SCREENING THE MAIN COMPONENTS RESPONSIBLE FOR BIOACTIVITY

Abstract

Herein, the angiotensin I-converting enzyme (ACE) inhibitory activity, antioxidant capacity, total polyphenol contents (TPC), and phytochemical profiles of polar and non-polar extracts of dried Ziziphus jujuba fruits were investigated, along with the statistical determination of the main components responsible for ACE inhibitory activity. The non-polar extract expressed the strongest ACE inhibitory activity (99.81%) among the extracts. The non-polar extract also exhibited the highest DPPH scavenging activity (IC50 of 30.63), linoleic acid/β-carotene bleaching capacity (89.31%), and TPC (59.47 mg GAE/g). The phenolic profiles of the extracts were identified by LC-MS/MS, and the presence of seven triterpenoid species in the extracts was examined using GC-MS techniques. The principal constituents included 19 phenolics, 2 organic acids, and 4 triterpenoids. A Pearson correlation and principal component analysis were conducted to find the correlation between individual phenolic compounds and ACE inhibitory activity.

Keywords

• Ziziphus jujuba
• ACE inhibitory activity
• antioxidant capacity
• phytochemical profile
• Pearson correlation
• principal component analysis

ZIZIPHUS JUJUBA (HÜNNAP) MEYVESİNİN POLAR VE APOLAR EKSTRAKLARININ ACE İNHİBİTÖR AKTİVİTESİ, ANTİOKSİDAN KAPASİTESİ VE FİTOKİMYASAL BİLEŞENLERİNİN İNCELENMESİ: BİYOAKTİVİTEDEN SORUMLU ANA BİLEŞENLERİN İSTATİSTİKSEL İNCELENMESİ

Abstract

Bu çalışmada Ziziphus jujuba’nın kurutulmuş meyvelerinin polar ve apolar ekstraktlarının anjiyotensin I-dönüştürücü enzim (ACE) inhibitör aktivitesi, antioksidan kapasitesi, toplam polifenol içerikleri (TPC) ve fitokimyasal profilleri araştırılmıştır ve ACE inhibitör aktivitesinden sorumlu temel bileşenler istatistiksel olarak analiz edilmiştir. En yüksek ACE inhibitör aktivitesi (%99.81) meyvenin apolar ekstraktında tespit edilmiştir. Apolar ekstrakt ayrıca en yüksek DPPH radikal süpürme aktivitesi (IC50 : 30,63), linoleik asit/β-karoten ağartma kapasitesi (%89.31) ve TPC'yi (59.47 mg GAE/g) sergilemiştir. Ekstraktların fenolik profilleri LC-MS/MS ile tanımlanmış ve ekstraktlardaki yedi triterpenoid türünün varlığı GC-MS teknikleri kullanılarak incelenmiş ve 19 fenolik, 2 organik asit ve 4 triterpenoid tanımlanmıştır. ACE inhibisyon aktivitesinden sorumlu fenolik bileşenlerin belirlenmesi amacı ile Pearson korelasyonu ve temel bileşen analizi kullanılmıştır.

Keywords

• Ziziphus jujuba
• ACE inhibitory activity
• antioxidant capacity
• phytochemical profile
• Pearson correlation
• principal component analysis

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