YOĞURDUN UÇUCU BİLEŞEN PROFİLİ VE DUYUSAL ÖZELLİKLERİ ÜZERİNE KÜLTÜR ÇEŞİDİNİN ETKİLERİ

Abstract

Çalışmada, yoğurt üretiminde farklı kültür çeşitleri kullanımının yoğurdun bazı kimyasal ve duyusal özellikleri ile uçucu bileşenleri üzerine etkisinin belirlenmesi amaçlanmıştır. Bu amaçla altı farklı ticari kültür kullanılmıştır. Yoğurt örneklerinin protein, kül, titrasyon asitliği, laktoz ve tirozin değerlerinin sırasıyla %4.13-4.38, %1.10-1.21, %0.92-1.23, %5.62-6.19 ve 9.57-14.76 mg tirozin/100 g yoğurt arasında değiştiği ortaya konmuştur. Uçucu bileşenlerin belirlenmesi amacıyla katı faz mikroekstraksiyon tekniği ve Gaz Kromatografisi-Kütle Spektrometresi kullanılmıştır. Analiz sonucunda asetaldehit, diasetil, asetoin, asetik asit, bütanoik asit ve hekzanoik asit yoğurtların karakteristik uçucu bileşenleri olarak saptanmıştır. Tanımlayıcı duyusal analizler sonucunda topaklı yapı, kıvam, sünen yapı, fermente ve ekşi terimleri panelistlerce en yoğun algılanan duyusal özellikler olarak belirlenmiştir.

Keywords

• yoğurt
• kültür
• uçucu bileşen

EFFECTS OF CULTURE TYPE ON VOLATILE COMPONENT PROFILE AND SENSORY PROPERTIES OF YOGURT

Abstract

In this study, it was aimed to determine the effect of using different varieties of cultures in yogurt production on some chemical, sensory properties and volatile compounds. Six different starter cultures were used for this purpose. It was revealed that the protein, ash, titratable acidity, lactose and tyrosine values of yoghurts varied between 4.13-4.38%, 1.10-1.21%, 0.92-1.23%, 5.62-6.19% and 9.57-14.76 mg tyrosine/100g yogurt, respectively. Solid phase microextraction technique and Gas Chromatography-Mass Spectrometry were used to determine volatile components. Acetaldehyde, diacetyl, acetoin, acetic acid, butanoic acid and hexanoic acid were determined as the characteristic volatile compounds of yoghurts. As a result of descriptive sensory analysis, the terms lumpy structure, consistency, ropy structure, fermented and sour were determined as the most intensely perveived sensory characteristics by the panelists.

Keywords

• yogurt
• culture
• volatile component
• sensory

References

1. Akan, E., Yerlikaya, O., Saygılı, D., Kınık, Ö. (2021). Farklı starter kültür kullanımının yoğurtların tekstürel ve viskozite özelliklerine etkisi. Ege Üniversitesi Ziraat Fakültesi Dergisi, 58(3), 377-383.
2. AOAC (2000). Official methods of analysis of AOAC International. Gaithersburg, USA, https://doi.org/10.3109/15563657608988149.
3. Avsar Y. K., Karagül Yüceer Y., Drake M. A., Singh T. K., Yoon Y., Cadwallader K.R. (2004). Characterization of nutty flavor in cheddar cheese. Journal of Dairy Science, 87: 1999-2010.
4. Beshkova, D., Simova, E., Frengova, G., Simov, Z. (1998). Production of flavour compounds by yogurt starter cultures. Journal of Industrial Microbiology and Biotechnology, 20(3): 180-186.
5. Cheng, H. (2010). Volatile flavor compounds in yogurt: A review. Critical Reviews in Food Science and Nutrition, 50(10): 938-950.
6. Clarke, H., Fitzpatrick, E., Hennessy, D., O'Sullivan, M. G., Kerry, J. P., Kilcawley, K. N. (2022). The influence of pasture and non-pasture-based feeding systems on the aroma of raw bovine milk. Frontiers in Nutrition, 9: 841454.
7. Dias, P.G.I., Sajiwani, J.W.A., Rathnayaka, R.M. (2020). Consumer perception and sensory profile of probiotic yogurt with added sugar and reduced milk fat. Heliyon, 6(7): e04328.
8. Drake, M. A., Karagül-Yüceer, Y., Chen, X. Q., Cadwallader, K. R. (1999). Characterization of desirable and undesirable lactobacilli from cheese in fermented milk. LWT-Food Science and Technology, 32(7): 433-439.

9. Gursoy, A., Durlu-Özkaya, F., Yıldız F., Aslım, B. (2010). Set Type Yoghurt Production by Exopolysaccharide Producing Turkish Origin Domestic Strains of Streptococcus thermophilus (W22) and Lactobacillus delbrueckii ssp. bulgaricus. Kafkas Üniversitesi Veteriner Fakültesi Dergisi, 16(1): 81-86.
10. Güler-Akın, M.B., Akın, M.S., Korkmaz, A. (2009). Influence of different exopolysaccharide‐producing strains on the physicochemical, sensory and syneresis characteristics of reduced‐fat stirred yoghurt. International Journal of Dairy Technology, 62(3): 422-430.
11. Gürsoy, A., Durlu-Özkaya, F., Yıldız, F., Aslım, B. (2010). Set type yoghurt production by exopolysaccharide producing Turkish origin domestic strains of Streptococcus thermophilus (W22) and Lactobacillus delbrueckii ssp. bulgaricus (B3). Kafkas Universitesi Veteriner Fakültesi Dergisi, 16: 81-86.
12. Güven, M. ve Karaca, O. B. (2003). Farklı yöntemlerle kurumaddesi artırılan sütlerden üretilen yoğurtların özellikleri. Gıda, 28(4): 429-436.
13. Güzel-Seydim, Z. B., Sezgin, E., Seydim, A. C. (2005). Influences of exopolysaccharide producing cultures on the quality of plain set type yogurt. Food Control, 16(3): 205-209.
14. Heo, J., Lee, S.J., Oh, J., Kim, M.R., Kwak, H.S. (2023). Comparison of descriptive analysis and flash profile by naïve consumers and experts on commercial milk and yogurt products. Food Quality and Preference, 110, 104946.
15. Hull, M. E. (1947). Studies on milk proteins. 2. colorimetric determination of the partial hydrolysis of the proteins in milk. Journal of Dairy Science, 30: 881-884
16. Isleten, M., Karagul-Yuceer, Y. (2006). Effects of dried dairy ingredients on physical and sensory properties of nonfat yogurt. Journal of Dairy science, 89(8): 2865-2872.
17. Kırdar, S. S. (2019). Süt ve Ürünlerinde Laboratuvar Uygulamaları Analiz Yöntemleri. Sidas Medya: İzmir.
18. Kneifel, W., Ulberth, F., Erhard, F., Jaros, D. (1992). Aroma profiles and sensory properties of yogurt and yogurt-related products. I: Screening of commercially available starter cultures. Milchwissenschaft, 47(6): 362-365.
19. Köse, Ş., Ocak, E. (2014). Yoğurtta lezzet bileşenlerinin oluşumu ve bu oluşum üzerine etki eden faktörler. Akademik Gıda, 12(2): 101-107.
20. Krastanov, A., Yeboah, P.J., Wijemanna, N.D., Eddin, A.S., Ayivi, R.D., Ibrahim, S.A. (2023). Volatile aromatic flavor compounds in yogurt: a review. Current Issues and Advances in the Dairy Industry. IntechOpen.
21. Marshall, V.M. (1993). Starter cultures for milk fermentation and their characteristics. International Journal of Dairy Technology, 46(2): 49-56.
22. Meilgaard M., Civille G.V., Carr B.T., (1999). Descriptive Analysis Techniques: Sensory Evaluation Techniques. 3. Edition Crc Press, Inc. Boca Raton, Fl. 161-172.
23. Moineau-Jean, A., Raymond, Y., Sabik, H., Graveline, N., Champagne, C. P., Roy, D., LaPointe, G. (2020). Effect of manufacturing processes and storage on aroma compounds and sensory properties of yoghurt. International Dairy Journal, 105: 104662.
24. NIST, (2008). NIST/EPA/NIH Mass Spectral Library (NIST 08). National Institute of Standards and Technology. Gaithersburg, M.D 20899.
25. Oyeniran, A., Gyawali, R., Aljaloud, S. O., Krastanov, A., Ibrahim, S. A. (2020). Probiotic characteristics and health benefits of the yogurt bacterium Lactobacillus delbrueckii sp. bulgaricus. In Current Issues and Challenges in the Dairy Industry. IntechOpen.
26. Özdemir, S., Bodur A.E. (1994). Yoğurt üretimi sırasında oluşan fiziksel, kimyasal ve biyokimyasal olaylar. Atatürk Üniversitesi Ziraat Fakültesi Dergisi, 25(3): 479- 487.
27. Özer, B. (2006). Yoğurt Bilimi ve Teknolojisi, Sidas Medya: İzmir.
28. Panesar, P.S. (2011). Fermented dairy products: starter cultures and potential nutritional benefits. Food and Nutrition Sciences, 2: 47-51.
29. Tian, H., Shi, Y., Zhang, Y., Yu, H., Mu, H., Chen, C. (2019). Screening of aroma‐producing lactic acid bacteria and their application in improving the aromatic profile of yogurt. Journal of Food Biochemistry, 43(10): e12837.
30. Ulberth, F. (1991). Headspace gas chromatographic estimation of some yogurt volatiles. Journal of the Association of Official Analytical Chemists, 74(4): 630- 634.
31. Ulberth, F., Kneifel, W. (1992). Aroma profiles and sensory properties of yogurt and yogurt-related products. II. Classification of starter cultures by means of cluster analysis. Milchwissenschaft 47: 432–434.
32. WILEY, (2005). Wiley Registry of Mass Spectral Data 7. Edition (F. W. Mclafferty) ISBN: 978-0471473251. Yaygın, H. (1999). Yoğurt Yapımında Saf Kültür Kullanımı ve Önemi III. Milli Süt ve Süt Ürünleri Sempozyumu, 2-3 Haziran, Ankara, 83-94.
33. Yiğit, S. ve Mendeş, M. (2018). Which effect size measure is appropriate for one-way and two-way ANOVA models? A Monte Carlo simulation study. Revstat-Statistical Journal, 16(3): 295-313.