EXTRACTION CONDITIONS FOR DIFFERENT PARTS OF MELOCAN (SMILAX EXCELSA L.) USING SUPERCRITICAL CARBON DIOXIDE

Abstract

Melocan, which is very important in both traditional Turkish cuisine and traditional medicine, especially in the Black Sea region of Türkiye, is a climbing, thorny and perennial plant that grows naturally in the bushy woodlands. In the study, different melocan parts (sprout, fruit and leaf) have been extracted under two different pressure levels (250 and 350 bar), temperatures (30 and 50 °C), durations (60 and 90 minutes), and two different levels of ethanol concentration (10% and 20%) through supercritical carbon dioxide (Sc-CO2) extraction method, which is an environmentally-friendly method. It has been observed that the melocan leaf which has been extracted through Sc-CO2 at 350 bar pressure and 50 °C which has been modified with 20% ethanol for 90 mins has the richest total phenolic substance content (TPC) as 2265.1 mg gallic acid equivalent (GAE)/kg dry weight and total antioxidant capacity (TAC) as 2876.7 mg trolox equivalent (TE)/kg dry weight.

Keywords

• Supercritical carbondioxide
• extraction conditions
• natural antioxidant
• melocan
• Smilax excelsa

MELOCAN'IN (SMILAX EXCELSA L.) FARKLI KISIMLARI İÇİN SÜPERKRİTİK KARBON DİOKSİT EKSTRAKSİYON KOŞULLARI

Abstract

Özellikle Türkiye’nin Karadeniz bölgesinde hem geleneksel mutfak kültüründe hem de geleneksel tıpta oldukça önemli yere sahip olan melocan, ormanlık arazilerin çalılık bölgelerinde kendiliğinden yetişen tırmanıcı, dikenli ve çok yıllık bir bitkidir. Bu çalışmada çevre dostu bir yöntem olan süperkritik karbon dioksit (Sc-CO2) ekstraksiyon yöntemi ile melocan bitkisinin farklı kısımları (filiz, meyve ve yaprak); iki farklı basınç (250 ve 350 bar), sıcaklık (30 ve 50 °C), süre (60 ve 90 dakika (dk)) ve iki farklı etanol konsantrasyonunda (%10 ve %20) ekstrakte edilmiştir. 350 bar basınç ve 50 °C'de, %20 etanol ile 90 dk Sc-CO2 ekstraksiyonuna tabii tutulan melocan yaprağının; 2265.1 mg gallik asit eşdeğeri (GAE)/kg kuru ağırlık olarak toplam fenolik madde (TFM) ve 2876.7 mg troloks eşdeğeri (TE)/kg kuru ağırlık olarak toplam antioksidan kapasite (TAK) açısından en iyi sonucu verdiği belirlenmiştir.

Keywords

• Süperkritik karbondioksit
• doğal antioksidanlar
• ekstraksiyon koşulları
• melocan
• Smilax excelsa

References

1. Akdeniz, B., Şümnü, G., Şahin, S. (2018). Microencapsulation of phenolic compounds extracted from onion (Allium cepa) skin. Journal of Food Processing and Preservation, 42, 13648, doi:10.1111/jfpp.13648.
2. Albuquerque, B.R., Heleno, S.A., Oliveira, M.B.P., Barros, L., Ferreira, I.C. (2021). Phenolic compounds: Current industrial applications, limitations and future challenges. Food & Function, 12(1): 14-29, doi:10.1039/D0FO02324H.
3. Al Yassine, D., El Massri, N., Demircan, G., Bulut, G., Akin, D., Tacer-Caba, Z. (2023). Total antioxidant potential, total phenolic profile and cytotoxic activity against brain cancer: Melocan and Galdirik. Food Technology and Biotechnology, 61(4): 475-484, doi:10.17113/ ftb.61.04.23.8071.
4. Arumugham, T., Rambabu, K., Hasan, S.W., Show, P.L., Rinklebe, J., Banat, F. (2021). Supercritical carbon dioxide extraction of plant phytochemicals for biological and environmental applications–A review. Chemosphere, 271, 129525, doi: 10.1016/j.chemosphere.2020.129525.
5. Aziz, M.A., Diab, A.S., Mohammed, A.A. (2019). Antioxidant categories and mode of action. In Antioxidants, Shalaby, E. (ed.), IntechOpen, doi:10.5772/intechopen.83544.
6. Bagchi, K., Puri, S. (1998). Free radicals and antioxidants in health and disease. East Mediterranean Health Jr, 4(2): 350-360.
7. Bimakr, M., Rahman, R.A., Taip, F.S., Ganjloo, A., Salleh, L.M., Selamat, J., Hamid, A., Zaidul, I. (2011). Comparison of different extraction methods for the extraction of major bioactive flavonoid compounds from spearmint (Mentha spicata L.) leaves. Food and Bioproducts Processing, 89(1): 67-72, doi:10.1016/j.fbp.2010.03.002.
8. Bitencourt, R.G., Queiroga, C.L., Duarte, G.H.B., Eberlin, M.N., Kohn, L.K., Arns, C.W., Cabral, F.A. (2014). Sequential extraction of bioactive compounds from Melia azedarach L. in fixed bed extractor using CO2, ethanol and water. The Journal of Supercritical Fluids, 95, 355-363, doi:10.1016/j.supflu.2014.09.027

9. Box, G.E.P., Hunter, W.H., Hunter, S. (1978). Statistics for experimenters: An Introduction to Design, Data Analysis and Model Building, John Wiley & Sons, Inc., New York. ISBN 0-471-09315-7
10. Carocho, M., Ferreira, I.C.F.R. (2013). A review on antioxidants, prooxidants and related controversy: Natural and synthetic compounds, screening and analysis methodologies and future perspectives. Food and Chemical Toxicology, 51, 15-25, doi:10.1016/j.fct.2012.09.021.
11. Da Porto, C., Natolino, A., Decorti, D. (2014). Extraction of proanthocyanidins from grape marc by supercritical fluid extraction using CO2 as solvent and ethanol-water mixture as co-solvent. The Journal of Supercritical Fluids, 87, 59-64, doi:10.1016/j.supflu.2013.12.013.
12. Da Silva, R.P., Rocha-Santos, T.A., Duarte, A.C. (2016). Supercritical fluid extraction of bioactive compounds. TrAC Trends in Analytical Chemistry, 76, 40-51, doi:10.1016/j.trac.2015.11.013.
13. De Castro, M. D. L., Priego-Capote, F. (2010). Soxhlet extraction: Past and present panacea. Journal of Chromatography A, 1217(16): 2383-2389, doi:10.1016/j.chroma.2009.11.027.
14. Dehghan, H., Sarrafi, Y., Salehi, P. (2016). Antioxidant and antidiabetic activities of 11 herbal plants from Hyrcania region, Iran. Journal Of Food and Drug Analysis, 24(1): 179-188, doi:10.1016/j.jfda.2015.06.010.
15. Estévez, M., Li, Z., Soladoye, O.P., Van-Hecke, T. (2017). Chapter two: Health risks of food oxidation. In Advances in Food and Nutrition Research, Toldrá, F (ed.), Volume 82, Academic Press, pp. 45-81, doi:10.1016/ bs.afnr.2016.12.005.
16. Foy, C.J., Passmore, A.P., Vahidassr, M.D., Young, I.S., Lawson, J.T. (1999). Plasma chain-breaking antioxidants in Alzheimer’s disease, vascular dementia and Parkinson’s disease. Quarterly Journal of Medicine, 92(1): 39-45, doi:10.1093/qjmed/92.1.39.
17. Ghafoor, K., Al-Juhaimi, F.Y., Choi, Y.H. (2012). Supercritical fluid extraction of phenolic compounds and antioxidants from grape (Vitis labrusca B.) seeds. Plant Foods for Human Nutrition, 67, 407-414, doi:10.1007/s11130-012-0313-1.
18. Hamburger, M., Baumann, D., Adler, S. (2004). Supercritical carbon dioxide extraction of selected medicinal plants-effects of high pressure and added ethanol on yield of extracted substances. Phytochemical Analysis: An International Journal of Plant Chemical and Biochemical Techniques, 15(1): 46-54, doi:10.1002/pca.743.
19. Ho, C.T. (1992). Phenolic compounds in food: An overview. In Phenolic Compounds in Food and Their Effects on Health II, Huang, M., Ho, C., Lee C. (eds.), Volume 2, ACS Symposium Series; American Chemical Society: Washington, DC, pp. 2-7. ISBN: 9780841224759.
20. Huang, W.Y., Cai, Y.Z., Zhang, Y. (2010). Natural phenolic compounds from medicinal herbs and dietary plants: Potential use for cancer prevention. Nutrition and Cancer, 62(1): 1-20, doi:10.1080/01635580903191585.
21. Ignat, I., Volf, I., Popa, V.I. (2011). A critical review of methods for characterisation of polyphenolic compounds in fruits and vegetables. Food Chemistry, 126(4): 1821-1835, doi:10.1016/j.foodchem.2010.12.026. Ivanova, A., Mikhova, B., Kostova, I., Evstatieva, L. (2010). Bioactive chemical constituents from Smilax excelsa. Chemistry of Natural Compounds, 46(2): 295-297.
22. Jahromi, S.G. (2019). Extraction techniques of phenolic compounds from plants. In Plant Physiological Aspects of Phenolic Compounds, Soto-Hernández, M., García-Mateos, R., Palma-Tenango, M (eds.), IntechOpen, doi:10.5772/intechopen.84705.
23. Jankovic, A., Chaudhary, G., Goia, F. (2021). Designing the design of experiments (DOE)–An investigation on the influence of different factorial designs on the characterization of complex systems. Energy and Buildings, 250, 111298, doi:10.1016/j.enbuild.2021.111298.
24. Koocheki, A., Taherian, A.R., Razavi, S.M., Bostan, A. (2009). Response surface methodology for optimization of extraction yield, viscosity, hue and emulsion stability of mucilage extracted from Lepidium perfoliatum seeds. Food Hydrocolloids, 23(8): 2369-2379, doi:10.1016/ j.foodhyd.2009.06.014.
25. Li, R., Xia, Z., Li, B., Tian, Y., Zhang, G., Li, M., Dong, J. (2021). Advances in supercritical carbon dioxide extraction of bioactive substances from different parts of Ginkgo biloba L. Molecules, 26(13): 4011, doi:10.3390/molecules26134011.
26. Lü, J., Lin, P.H., Yao, Q., Chen, C. (2010). Chemical and molecular mechanisms of antioxidants: experimental approaches and model systems. Journal of Cellular and Molecular Medicine, 14(4): 840-860, doi:10.1111/j.1582-4934.2009.00897.x.
27. Mandana, B., Russly, A., Ali, G., Farah, S. (2011). Antioxidant activity of spearmint (Mentha spicata L.) leaves extracts by Supercritical Carbon Dioxide (SC-CO2) extraction. International Food Research Journal, 18(2): 543-547.
28. Maran, J.P., Manikandan, S., Priya, B., Gurumoorthi, P. (2015). Box-Behnken design based multi-response analysis and optimization of supercritical carbon dioxide extraction of bioactive flavonoid compounds from tea (Camellia sinensis L.) leaves. Journal of Food Science and Technology, 52(1): 92-104, doi:10.1007/s13197-013-0985-z.
29. Mitić, M., Tošić, S., Pavlović, A., Mašković, P., Kostić, D., Mitić, J., Stevanović, V. (2019). Optimization of the extraction process of minerals from Salvia officinalis L. using factorial design methodology. Microchemical Journal, 145, 1224-1230, doi:10.1016/j.microc.2018.12.047.
30. Monroy, Y.M., Rodrigues, R.A., Sartoratto, A., Cabral, F.A. (2016a). Extraction of bioactive compounds from cob and pericarp of purple corn (Zea mays L.) by sequential extraction in fixed bed extractor using supercritical CO2, ethanol, and water as solvents. The Journal of Supercritical Fluids, 107, 250-259, doi:10.1016/j.supflu.2015.09.020.
31. Monroy, Y.M., Rodrigues, R.A., Sartoratto, A., Cabral, F.A. (2016b). Influence of ethanol, water, and their mixtures as co-solvents of the supercritical carbon dioxide in the extraction of phenolics from purple corn cob (Zea mays L.). The Journal of Supercritical Fluids, 118, 11-18, doi:10.1016/j.supflu.2016.07.019.
32. Noordin, M.Y., Venkatesh, V., Sharif, S., Elting, S., Abdullah, A. (2004). Application of response surface methodology in describing the performance of coated carbide tools when turning AISI 1045 steel. Journal of Materials Processing Technology, 145(1): 46-58, doi:10.1016/S0924-0136(03)00861-6.
33. Ozsoy, N., Can, A., Yanardag, R., Akev, N. (2008). Antioxidant activity of Smilax excelsa L. leaf extracts. Food Chemistry, 110(3): 571-583, doi:10.1016/j.foodchem.2008.02.037.
34. Özbucak, T.B., Akçin, Ö.E., Yalçın, S. (2007). Nutrition contents of the some wild edible plants in central black sea region of Turkey. International Journal of Natural and Engineering Sciences, 1, 11-13.
35. Rahmawati, A., Pang, D., Ju, Y., Soetaredjo, F.E., Ki, O.L., Ismadji, S. (2015). Supercritical CO2 extraction of phytochemical compounds from Mimosa pudica Linn. Chemical Engineering Communications, 202(8): 1011-1017, doi:10.1080/ 00986445.2014.896346.
36. Sajadian, S.A., Peyrovedin, H., Zomorodian, K., Khorram, M. (2023). Using the supercritical carbon dioxide as the solvent of Nystatin: Studying the effect of co-solvent, experimental and correlating. The Journal of Supercritical Fluids, 194, 105858, doi:10.1016/ j.supflu.2023.105858.
37. Sarıaltın, S.Y., Polat, D.C., Yalçın, C.Ö. (2023). Cytotoxic and antioxidant activities and phytochemical analysis of Smilax excelsa L. and Aegopodium podagraria L. Food Bioscience, 52, 102359, doi:10.1016/j.fbio.2023.102359.
38. Shahidi, F., Naczk, M. (2003). Phenolics in food and nutraceuticals. CRC Press google schola, 558 p. ISBN:9780367395094.
39. Sies, H. (1986). Biochemistry of oxidative stress. Angewandte Chemie International Edition in English, 25(12): 1058-1071, doi:10.1002/anie.198610581.
40. Silva, B.A., Ferreres, F., Malva, J.O., Dias, A.C.P. (2005). Phytochemical and antioxidant characterization of Hypericum perforatum alcoholic extracts. Food Chemistry, 90(1-2): 157-167, doi:10.1016/j.foodchem.2004.03.049.
41. Şahin, Ö. (2019). Melocan (Smilax Excelsa L.) bitkisinin farklı kısımlarının ultrason ve mikrodalga destekli ekstraksiyon ile elde edilen bileşenlerinin tanımlanması. Hacettepe Üniversitesi Fen Bilimleri Enstitüsü Gıda Mühendisliği Anabilim Dalı Yüksek Lisans Tezi, Ankara, Türkiye, 125 s.
42. Tanker, N., Koyuncu, M., Coşkun, M. (1993). Farmasötik Botanik Ders Kitabı. Ankara Üniversitesi Eczacılık Fakültesi Yayınları, Ankara, Türkiye, 449 p.
43. Topdas, E.F., Demirbaş, M., Şengül, M., Şat, İ.G. (2021). Farklı kurutma tekniklerinin Smilax excelsa genç sürgünlerinin antioksidan aktivitesi ve bazı fizikokimyasal özellikleri üzerine etkisi. Atatürk Üniversitesi Ziraat Fakültesi Dergisi, 52(3): 314-324, doi:10.17097/ataunizfd.816887.
44. Uwineza, P.A., Gramza-Michałowska, A., Bryła, M., Waśkiewicz, A. (2021). Antioxidant activity and bioactive compounds of lamium album flower extracts obtained by supercritical fluid extraction. Applied Sciences, 11(16): 7419, doi:10.3390/app11167419.
45. Uzombah, T.A. (2022). The implications of replacing synthetic antioxidants with natural ones in the food systems. In Natural food additives, Prieto, M.A., Otero, P. (eds.), IntechOpen, doi:10.5772/intechopen.103810.
46. Wang, Y., Ying, L., Sun, D., Zhang, S., Zhu, Y., Xu, P. (2011). Supercritical carbon dioxide extraction of bioactive compounds from Ampelopsis grossedentata stems: Process optimization and antioxidant activity. International Journal of Molecular Sciences, 12(10): 6856-6870, doi:10.3390/ijms12106856.
47. Wang, W., Rao, L., Wu, X., Wang, Y., Zhao, L., Liao, X. (2021). Supercritical carbon dioxide applications in food processing. Food Engineering Reviews, 13, 570-591, doi:10.1007/s12393-020-09270-9.
48. Xu, D., Li, Y., Meng, X., Zhou, T., Zhou, Y., Zheng, J., Zhang, J., Li, H. (2017). Natural antioxidants in foods and medicinal plants: Extraction, assessment and resources. International Journal of Molecular Sciences, 18(1) 96, doi:10.3390/ijms18010096.
49. Yeşilada, E., Sezik, E., Honda, G., Takaishi, Y., Takeda, Y., Tanaka, T. (1999). Traditional medicine in Turkey IX: Folk medicine in north-west Anatolia. Journal of Ethnopharmacology, 64(3): 195-210, doi:10.1016/S0378-8741(98)00133-0.
50. Yıldız, Ö.Ş., Ayanoğlu, F., Bahadırlı, N.P. (2018). Some morphological and chemical characteristics of sarsaparilla (Smilax aspera L., Smilax excelsa L.). Mustafa Kemal Üniversitesi Ziraat Fakültesi Dergisi, 23(2): 254-261. ISSN:1300-9362.