THE RELATIONSHIP BETWEEN ANTIOXIDANT ACTIVITIES AND PHENOLIC COMPOUNDS OF THE SUBCRITICAL WATER EXTRACTS FROM ORANGE PEEL

Abstract

Subcritical water extraction, which is used to extract phytochemicals from different plants and foods, is also called green technology. Orange peel, which a rich source of antioxidant compounds such as phenolics, β-carotene and essential oils, is a valuable by-product in the food industry. In this study, the determination of the relationship between antioxidant activitiy and phenolic compounds of subcritical water extracts obtained from orange peel was aimed. For this aim, The Ferric Reducing Antioxidant Assay (FRAP), The Cupric Reducing Antioxidant Capacity (CUPRAC) and DPPH radical scavenging activity (IC₅₀) of subcritical water extracts were measured. Total phenolic content of the subcritical water extracts from orange peel was determined statistically significant (p<0.01) correlated as R²=0.98 and 0.92 with FRAP and CUPRAC values. Also, total flavonoid content of the subcritical water extracts from orange peel had very high significant (P<0.01) positive correlations as R²=0.97 and 0.93 with FRAP and CUPRAC values. However, total phenolic and total flavonoid content in the subcritical water extracts from orange peel were statistically significant (p<0.01) correlated as R²=-0.68 and -0.70 with IC₅₀, respectively.

Keywords

• Antioxidant activity,DPPH,orange peel,subcritical water extraction,phenolics

PORTAKAL KABUĞUNDAN ELDE EDİLEN KRİTİK ALTI SU EKSTRAKTLARINDA ANTİOKSİDAN AKTİVİTE DEĞERLERİ ile FENOLİK BİLEŞİKLER ARASINDAKİ İLİŞKİ

Abstract

Farklı bitkilerden ve gıdalardan fitokimyasal bileşikleri ekstrakte etmek amacıyla kullanılan kritik altı su ekstraksiyonu aynı zamanda yeşil teknoloji olarak da adlandırılmaktadır. Fenolik bileşikler, β-karoten ve esansiyel yağlar gibi antioksidan bileşikler açısından zengin bir kaynak olan portakal kabuğu gıda endüstrisinde değerli bir yan üründür. Bu çalışmada, portakal kabuğundan elde edilen kritik altı su ekstraktlarında antioksidan aktivite değerleri ile fenolik bileşikler arasındaki ilişkinin saptanması amaçlanmıştır. Bu amaçla, kritik altı su ekstraktlarının Demir indirgeme Gücü Aktivitesi (FRAP), Bakır indirgeme Gücü Aktivitesi (CUPRAC) ve DPPH radikal süpürme aktivitesi (IC₅₀) ölçülmüştür. Portakal kabuğundan elde edilen kritik altı su ekstraktların toplam fenolik madde içeriği ile FRAP ve CUPRAC değerleri arasındaki ilişki istatistiksel (P<0.01) açıdan önemli olup sırası ile R²=0.98 ve 0.92 düzeylerinde bulunmuştur. Aynı zamanda, portakal kabuğundan elde edilen kritik altı su ekstraktların toplam flavonoid içeriği de FRAP ve CUPRAC değerleri ile istatistiksel (P<0.01) açıdan önemli düzeyde ilişkilidir (sırası ile R²=0.97 ve 0.93). Buna karşın, portakal kabuğundan elde edilen kritik altı su ekstraktların toplam fenolik madde ve toplam flavonoid içerikleri ile IC₅₀ değeri arasındaki ilişki sırası ile R²=-0.68 ve -0.70 düzeylerinde olup istatistiksel (P<0.01) açıdan önemlidir.

Keywords

• Antioksidan aktivite,DPPH,portakal kabuğu,kritik altı su ekstraksiyonu,fenolikler

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