DETECTION OF LISTERIA SPP. IN RAW MILK AND DAIRY PRODUCTS RETAILED IN ANKARA

Abstract

The objective of this study was to determine the prevalence of Listeria species in raw milk, and dairy products. A total of 110 samples were obtained from randomly selected retail stores and local bazaars located in Ankara. Using TS EN ISO 11290-1 method, 23 samples were found positive for Listeria spp. The overall prevalence of Listeria spp. was 20.91%, in which L. innocua was the most commonly recovered species (6.36%). The remaining isolates were identified as L ivanovii (5.45%), L. monocytogenes (4.55%), and L welshimeri (4.55%). The L monocytogenes isolates were positive for the presence of hlyA gene. The highest prevalence of Listeria spp. was found in homemade cheese (9.09%), followed by raw milk (8.19%), and white cheese (3.64%). L monocytogenes was isolated from raw milk and homemade cheese in this study.

In conclusion, the low hygienic quality dairy products may lead to listeriosis surveillance in Ankara. 

Keywords

• Listeria,Milk,Milk Products,Identification

ANKARA’DA SATIŞA SUNULAN ÇİĞ SÜT VE SÜT ÜRÜNLERİNDE LISTERIA SPP. VARLIĞININ BELİRLENMESİ

Abstract

Bu çalışmada çiğ süt ve süt ürünlerinde Listeria türlerinin yaygınlığının saptanması amaçlanmıştır. Ankara'da bulunan ve rastgele seçilen market ve pazarlardan toplam 110 örnek alınmıştır. TS EN ISO 11290-1 yöntemi kullanılarak, toplam 23 örnek Listeria spp. varlığı bakımından pozitif bulunmuştur. Analize alınan örneklerde Listeria türlerinin bulunma sıklığı %20.91 olarak tespit edilmiş, L. innocua (%6.36) ise en fazla rastlanan tür olarak belirlenmiştir. Geri kalan izolatlar ise L. ivanovii (%5.45), L. monocytogenes (%4.55) ve L. welshimeri (%4.55) olarak tanımlanmıştır. L. monocytogenes suşları hlyA gen bölgesi varlığı bakımından pozitif olarak saptanmıştır. En yüksek Listeria spp. prevalansı ise, sırasıyla ev yapımı peynir (%9.09), çiğ süt (%8.19) ve beyaz peynirde (%3.64) saptanmıştır. Bu çalışmada L. monocytogenes suşları çiğ süt ve ev yapımı peynirlerden izole edilmiştir.

Sonuç olarak hijyenik kalitesi düşük süt ürünlerinin,  listeriosis surveyansını Ankara’da artırabileceği gözlemlenmiştir.

Keywords

• Listeria,Süt,Süt Ürünleri,Tanımlama

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