DETECTION OF LISTERIA SPP. IN RAW MILK AND DAIRY PRODUCTS RETAILED IN ANKARA

Öz

The objective of this study was to determine the prevalence of Listeria species in raw milk, and dairy products. A total of 110 samples were obtained from randomly selected retail stores and local bazaars located in Ankara. Using TS EN ISO 11290-1 method, 23 samples were found positive for Listeria spp. The overall prevalence of Listeria spp. was 20.91%, in which L. innocua was the most commonly recovered species (6.36%). The remaining isolates were identified as L ivanovii (5.45%), L. monocytogenes (4.55%), and L welshimeri (4.55%). The L monocytogenes isolates were positive for the presence of hlyA gene. The highest prevalence of Listeria spp. was found in homemade cheese (9.09%), followed by raw milk (8.19%), and white cheese (3.64%). L monocytogenes was isolated from raw milk and homemade cheese in this study.

In conclusion, the low hygienic quality dairy products may lead to listeriosis surveillance in Ankara. 

Anahtar Kelimeler

• Listeria,Milk,Milk Products,Identification

ANKARA’DA SATIŞA SUNULAN ÇİĞ SÜT VE SÜT ÜRÜNLERİNDE LISTERIA SPP. VARLIĞININ BELİRLENMESİ

Öz

Bu çalışmada çiğ süt ve süt ürünlerinde Listeria türlerinin yaygınlığının saptanması amaçlanmıştır. Ankara'da bulunan ve rastgele seçilen market ve pazarlardan toplam 110 örnek alınmıştır. TS EN ISO 11290-1 yöntemi kullanılarak, toplam 23 örnek Listeria spp. varlığı bakımından pozitif bulunmuştur. Analize alınan örneklerde Listeria türlerinin bulunma sıklığı %20.91 olarak tespit edilmiş, L. innocua (%6.36) ise en fazla rastlanan tür olarak belirlenmiştir. Geri kalan izolatlar ise L. ivanovii (%5.45), L. monocytogenes (%4.55) ve L. welshimeri (%4.55) olarak tanımlanmıştır. L. monocytogenes suşları hlyA gen bölgesi varlığı bakımından pozitif olarak saptanmıştır. En yüksek Listeria spp. prevalansı ise, sırasıyla ev yapımı peynir (%9.09), çiğ süt (%8.19) ve beyaz peynirde (%3.64) saptanmıştır. Bu çalışmada L. monocytogenes suşları çiğ süt ve ev yapımı peynirlerden izole edilmiştir.

Sonuç olarak hijyenik kalitesi düşük süt ürünlerinin,  listeriosis surveyansını Ankara’da artırabileceği gözlemlenmiştir.

Anahtar Kelimeler

• Listeria,Süt,Süt Ürünleri,Tanımlama

Kaynakça

1. 1. Abay, S., Aydın, F., Sümerkand, A.B. (2012). Molecular typing of Listeria spp. isolated from different sources. Ankara Üniv. Vet. Fak. Derg., 59: 183-190.
2. 2. Abrahao, W.M., Abrahao, P.R.S., Monteiro, C.L.B., Pontarolo, R. (2008). Occurrence of Listeria monocytogenes in cheese and ice cream produced in the State of Prana, Brazil. Braz. J. Pharm. Sci., 44: 289-296.
3. 3. Akkaya, L., Alişarlı, M. (2006). Afyonkarahisar’da tüketime sunulan peynirlerde Listeria monocytogenes ve Salmonella spp. varlığının belirlenmesi. Y.Y.Ü. Vet. Fak.Derg., 17(1-2): 87-91.
4. 4. Al-Mariri, A., AbouYounes, A., Ramadan, L. (2013). Prevalence of Listeria spp. in Raw Milk in Syria. Bulg. J. Vet. Med., 16(2): 112-122.
5. 5. Anonymous. (2017). https://www.ncbi.nlm.nih.gov/Taxonomy/Browser, Access Date: (24.03.2017).
6. 6. Arrese, E., Arroyo-Izaga, M. (2012). Prevalence of Listeria monocytogenes in Idiazabal cheese. Nutr. Hosp., 27(6):2139-2141. doi: 10.3305/nh.2012.27.6.6052.
7. 7. Arslan, S., Özdemir, F. (2008). Prevalence and antimicrobial resistance of Listeria spp. in homemade white cheese. Food Control, 19: 360-363. Doi:10.1019/j.foodcont.2007.04. 009.
8. 8. Aygun, O., Pehlivanlar, S. (2006). Listeria spp. in the raw milk and dairy products in Antakya, Turkey. Food Control, 17: 676-679. Doi:10.1016/j.foodcont.2005.09.014.

9. 9. Azak, M. G., Kılıç, H., Hızlısoy, H., Abay, S. (2012). Erzincan ili tulum peynirlerinden Listeria spp. İzolasyonu ve İdentifikasyonu. Erciyes Üniv. Vet. Fak. Derg., 9(3): 149-156.
10. 10. Barre, L., Angelidis, A.S., Boussaid, D., Brasseur, E.D., Manso, E., Besse, N.G. (2016). Applicability of the EN ISO 11290-1 standard method for Listeria monocytogenes detection in presence of new Listeria species. Int. J. Food Microbiol., 238: 281-287. Doi: 10.1016/j.ijfoodmicr.2016.09.028.
11. 11. Beasley, S. S., Saris, P. E. J. (2004). Nisin-Producing Lactococcus lactis Strains Isolated from Human Milk. Applied and Environmental Microbiology, 5051-5053.
12. 12. Blaiotta, G., Pepe, O., Mauiello, G., Villani, F., Andolfi, R., Moschetti, G. (2002). 16S-23S rDNA intergenic spacer region polymorphism of Lactococcus gavriae, Lactococcus raffinolactis and Lactococcus lactis as revealed by PCR and nucleotide sequence analysis system. Applied and Environmental Microbiolology, 25: 520-527.
13. 13. Botsaris, G., Nikolaou, K., Liapi, M., Pipis, C. (2016). Prevalence of Listeria spp. and Listeria monocytogenes in cattle farms in Cyprus using bulk tank miilk samples. Journal of Food Safety, 36: 482-488. Doi: 10.1111/jfs.12265
14. 14. Coroneo, V., Carraro, V., Aissani, N., Sanna, A., Ruggeri, A., Succa, S., Meloni, B., Pinna, A., Sanna, C. (2016). Detection of Virulence Genes and Growth Potential in Listeria monocytogenes Strains Isolated from Ricotta Salata Cheese. Journal of Food Science, 81(1): M114-M120.
15. 15. Durmaz, H., Avcı, M., Aygün, O. (2015). The Presence of Listeria Species in Corn Silage and Raw Milk Produced in Southeast Region of Turkey. Kafkas Univ. Vet. Fak.Derg., 21:41-44. Doi: 10.9775/kvfd.2014.11664.
16. 16. Elshinaway, S.H., Meshref, A.M.S., Zeinhom, M.M.A., Hafez, D.A.A. (2017). Incidence of Listeria Species in Some Dairy Products Inbeni-Suef Governorate. Assiut. Vet. Med. J., 63: 5-13.
17. 17. Gebretsadik, S., Kassa, T., Alemayehu, H., Huruy, K., Kebede, N. (2011). Isolation and characterization of Listeria monocytogenes and other Listeria species in foods of animal origin in Addis Ababa, Ethiopia. J. Infect. Public Health, 4: 22-29. Doi:10.1016/j.jiph.2010.10.002.
18. 18. Gökmen, M., Akkaya, L., Kara, R., Önen, A. (2016). Prevalence of Salmonella spp. and L. monocytogenes in some ready to eat foods sold retail in Balıkesir. Van Vet. J., 27(1):31-36.
19. 19. Hellberg, R.S., Martin, K.G., Keys, A.L., Haney, C.J., Shen, Y., Smiley, R.D. (2013). 16S rRNA partial gene sequencing for the differentiation and molecular subtyping of Listeria species. Food Microbiol., 36: 231-240. Doi: 10.1016/j.fm.2013.06.001.
20. 20. ISO 11290-1:1996 Microbiology of food and animal feding stuffs-Horizontal method for the detection and enumeration of Listeria monocytogenes-Part 1: Detection method.
21. 21. Jamali, H., Chai, L.C., Thong, K.L. (2013). Detection and Isolation of Listeria spp. And Listeria monocytogenes in ready-to-eat foods with various selective culture media. Food Control, 32:19-24. Doi:10.1016/j.foodcont.2012.11.033.
22. 22. Kaptan, B. (2016). Prevalence of Listeria spp. and L. monocytogenes in Homemade Pottery Cheese. Tekirdağ Ziraat Fakültesi Dergisi. 13: 80-87.
23. 23. Kasalica, A., Vukovic, V., Vranjes, A., Memisi, N. (2011). Listeria monocytogenes in milk and dairy products. Biotechnology in Animal Husbandary, 27(3): 1067-1082. Doi:10.2298/BAH1103067K
24. 24. Kevenk, T. O. (2014). Süt ve Ürünlerinde Listeria monocytogenes’in İnsidensi, Serotiplendirilmesi ve Antibiyotik Dirençliliklerinin Belirlenmesi. Ondokuz Mayıs Üniversitesi Sağlık Bilimleri Enstitüsü Besin Hijyeni ve Teknolojisi Anabilim Dalı Doktora Tezi, Samsun, Türkiye, 116 sayfa.
25. 25. Kongo, J. M., Malcata, F.X., Ho, A. J., Wiedmann, M. (2006). Detection and Caharacterization of Listeria monocytogenes in Sao Jorge (Portugal) Cheese Production. J. Dairy Sci., 89: 4456-4461.
26. 26. Korsak, D., Szuplewska, M. (2016). Characterization on nonpathogenic Listeria species isolated from food and food processing environment. Int. J. Food Microbiol., 238: 274-280. Doi: 10.1016/j.ijfoodmicro.2016.08.032.
27. 27. Kum, E., Yıldırım, Y., Ertaş, N. (2011). Kayseri’de Satışa Sunulan Peynir Örneklerinde Listeria monocytogenes Varlığının Klasik Kültür Yöntemi ile Belirlenmesi. Erciyes Üniversitesi Veteriner Fakültesi Dergisi, 8(2): 105-109.
28. 28. Marnissi, B. E., Bennani, L., Cohen, N., Lalami, A.E. O., Belkhou, R. (2013). Presence of Listeria monocytogenes in raw milk and traditional dairy products marketed in the North-central region of Morocco. African Journal of Food Science, 7(5): 87-91. Doi: 10.5897/AJFS2013.0992.
29. 29. Milillo, S. R., Friendly, E. C., Saldivar, J. C., Muthaiyan, A., O’Brian, C., Crandall, P. G., Johnson, M. G., Ricke, S. C. (2012). A review of the ecology, genomics, and stres response of Listeria innocua and Listeria monocytognes. Critical Reviews in Food Science and Nutrition, 52: 712-725. Doi: 10.1080/10408398.2010.507909.
30. 30. Momtaz, H., Yadollahi, S. (2013). Molecular Characterization of Listeria monocytogenes isolated from fresh sea food samples in Iran. Diagn. Pathol., 8: 149.
31. 31. Moosavy, M. M., Esmaeili,S., Mostafavi, E., Amiri, F.B. (2014). Isolation of Listeria monocytogenes from milks used for Iranian traditional cheese in Lighvan cheese factories. Annals of Agricultural and Environmental Medicine. 21(4): 728-729.
32. 32. Natratilova,P., Schlegelova, J., Sustackova, A., Napravnikova, E., Lukasova, J. (2004). Prevalence of Listeria monocytogenes in milk, meat and foodstuff of animal origin and the phenotype of antibiotic resistance of isolated strains. Vet. Med. Czech, 49: 243-252.
33. 33. Odetokun, I.A., Adetunji, V.O. (2016). Prevalence and persistence of Listeria monocytogenes in dairy and other ready-to-eat food products in Africa. Microbes in Food and Health, 319: 349-361. Doi: 10.1007/978-3-319-25277-3-18.
34. 34. Paziak-Domanska, B., Bogulawska, E., Wiekowska-Szakiel, M., Kotlowski, R., Rozalska, B., Chmiela, M., Kur, J., Dabrowski, W., Rudnicka, W. (1999). Evaluation of the API test, phosphatidylinositol-specific phospholipase C activity and PCR method in identification of Listeria monocytogenes in meat foods. FEMS Microbiology Letters,171: 209–214.
35. 35. Phraephaisarn, C., Khumthong, R., Takahashi, H., Ohshima, C., Kodama, K., Techaruvichit, P., Vvesaratchavest, M., Taharnklaew, R., Keeratipibul, S. (2017). A novel biomarker for detection of Listeria species in food processing factory. Food Control, 73: 1032-1038. Doi:10.1016/j.foodcont.2016.10.001.
36. 36. Rahimi, E., Momtaz, H., Sharifzadeh, A., Behzadnia, A., Ashtari, M.S., Esfahani, S.Z., Riahi, M., Momeni, M. (2012). Prevalence and Antimicrobial Resistance of Listeria Species Isolated from Traditional Dairy Products in Chahar Mahal &Bakhtiyari, Iran. Bulgarian J. Vet. Med., 15: 115-122.
37. 37. Reda, W.W., Abdel-Moein, K., Hegazi, A., Mohamed, Y., Abdel-Razik, K. (2016). Listeria monocytogenes:An emerging food-borne pathogen and its public health implications. J. Infect. Dev. Ctries, 10(2), 149-154. Doi: 10.3855/jidc.6616
38. 38. Sağun, E., Sancak, Y.C., İşleyici, Ö., Ekici, K. (2001). Van ve Çevresi Süt ve Otlu Peynirlerinde Listeria Türlerinin Varlığı ve Yaygınlığı Üzerine Bir Araştırma. Turk. J. Vet. Anim. Sci., 25: 15-19.
39. 39. Saludes, M., Troncoso, M., Figuero, G. (2015). Presence of Listeria monocytogenes in Chilean food matrices. Food Control, 50: 331-335. Doi: 10.1016/j.foodcont.2014.08.008.
40. 40. Sarfaz, M., Ashraf, Y., Ashraf, S. (2017). A Review:Prevalence and antimicrobial susceptibility profile of Listeria species in milk. Matrix Science Medica.1(1): 03-09. Doi:10.26480/msm.01.2017.03.09.
41. 41. Sarker, R., Ahmed, S. (2015). Prevalence and Antimicrobial Susceptibility of Listeria spp in Dairy Food Products and Water Samples in Dhaka, Bangladesh. J. Life Sci., 9:152-158. Doi:10.17265/1934-7391/2015.04.002.
42. 42. Seyoum, E.T., Woldetsadik, D.A., Mekonen, T.K., Gezahegn, H.A., Gebreyes, W.A. (2015). Prevalence of Listeria monocytogenes in raw bovine milk and milk products from central highlands of Ethiopia. J. Infect Dev. Ctries., 9: 1204-1209. Doi:10.3855/jidc.6211.
43. 43. Silva, I.M.M., Almeida, R.C.C., Alves, M.A.O., Almedia, P.F. (2003). Occurrence of Listeria spp. in critical control points and the environment of Minas Frescal cheese processing. Int. J. Food. Microbiol., 81:241-248.
44. 44. Soni, D., Singh, R., Singh, D., Dubey, S. (2013). Characterization of Listeria monocytogenes isolated from Ganges water, human clinical and milk samples at Varanasi, India. Infection, Genetics and Evolution, 14: 83-91.
45. 45. Taşçı, F., Türütoğlu, H., Öğütçü, H. (2010). Investigations of Listeria Species in Milk and Silage Produced in Burdur Province. Kafkas Univ. Vet. Fak. Derg., 16:S93-S97. Doi:10.9775/kvfd.2010.1744.
46. 46. Telli, N., Güner, A., Dönmez, F.S., Özdemir, Ö.Ö. (2016). Detection of the contamination sources of Listeria monocytogenes in pickled white cheese production process line and genotyping with the pulsed-field gel electrophoresis method. Turk. J. Vet. Anim. Sci., 40, 630-636. Doi:10.3906/vet-1511-59
47. 47. Usman, U. B., Kwaga, J. K. P., Kabir, J., Olonitola, O.S. (2016). Isolation and antimicrobial suspectibility of Listeria monocytogenes from raw milk and milk products in Northern Kaduna State, Nigeria. Journal of Applied and Environmental Microbiology, 4(3): 46-54. Doi: 10.1269/jaem-4-3-1.
48. 48. Vardar-Ünlü, G., Ünlü, M., Bakıcı, M.Z.(1998). Incidence of Listeria spp from raw milk in Sivas. Tr. J. Medical Sci., 28: 389-392.
49. 49. Yehia, H.M., Ibraheim, S.M., Hassanein, W.A. (2016). Prevalence of Listeria species in some foods and their rapid identification. Trop. J. Pharm. Res., 15: 1047-1052. Doi: 10.4314/ tjpr.v15i5.21.