PROPOLİS EKSTRAKTI İLE ZENGİNLEŞTİRİLMİŞ BALIK YAĞINDA LİPİT OKSİDASYONU DÜZEYİNİN BELİRLENMESİ

Abstract

Bu çalışma, propolis ekstraktının balık yağında doğal antioksidan olarak kullanımı üzerine odaklanmıştır. Farklı konsantrasyonlarda (100, 500, 1.000 mg/kg)  propolis ekstraktı ve sentetik antioksidan olarak bütillendirilmiş hidroksitoluen (BHT, 100 mg/kg) balık yağına eklenerek hızlandırılmış oksidasyon koşulları altında lipit oksidasyonu düzeyi belirlenmiştir. Depolama süresince peroksit değeri, UV-spektrum değerleri (UV₂₃₂ ve UV₂₇₀), tiyobarbitürik asit (TBARS) değeri ve para-anisidin değeri incelenmiştir.  Depolama boyunca propolis ekstraktı ilaveli gruplarda peroksit değeri, UV₂₃₂ ve UV₂₇₀ değeri, TBARS değeri ve para-anisidin değeri kontrol grubu ve BHT ilaveli gruptan daha düşük düzeylerde bulunmuştur. Elde edilen sonuçlar, 500 ve 1.000 mg/kg konsantrasyonlarında propolis ekstraktı ilave edilmiş balık yağı örneklerinde oksidasyon düzeyinin daha düşük olduğunu ve en etkili konsantrasyonun 1.000 mg/kg olduğunu göstermektedir. Bu çalışma ile, propolis ekstraktının balık yağında oksidatif stabiliteyi sağlamada doğal bir antioksidan kaynağı olarak kullanılabileceği gösterilmektedir.

Keywords

• Balık yağı,propolis ekstraktı,lipit oksidasyonu,doğal antioksidan,oksidatif bozulma

DETERMINATION OF THE LIPID OXIDATION LEVEL IN FISH OIL ENRICHED WITH PROPOLIS EXTRACT

Abstract

This study focused on the utilization of propolis extract as a natural antioxidant in fish oil. Propolis extract at different concentrations (100, 500, 1,000 mg/kg) and butylated hydroxytoluene (BHT, 100 mg/kg) as synthetic antioxidant were added to fish oil in order to determine the lipid oxidation level under accelerated oxidation conditions. Peroxide value, UV-spectrum values (UV₂₃₂ and UV₂₇₀), thiobarbituric acid (TBARS) value and para-anisidine value were investigated during the storage. Peroxide value, UV₂₃₂ and UV₂₇₀ value, TBARS value and para-anisidine value of groups which supplemented with the propolis extract was found lower than the control group and BHT added group throughout the storage period. The results show that the oxidation level in fish oil samples supplemented with 500 and 1.000 mg/kg propolis extract lower, and the most effective concentration is 1.000 mg/kg propolis extract. This study indicated that the propolis extract might be used as a natural source in order to provide oxidative stability in fish oil.

Keywords

• Fish oil,propolis extract,lipid oxidation,natural antioxidant,oxidative deterioration

References

1. Abd El-aal, H.A., Halaweish, F.T. (2010). Food preservative activity of phenolic compounds in orange peel extracts (Citrus sinensis L.). Lucrări Ştiinţifice - Seria Zootehnie, 53(15): 233-240.
2. Alonso, I.S., Escrig, A.J., Calixo, F.S, Borderias, A.J. (2007). Effect of grape antioxidant dietary fibre on the prevention of lipid oxidation in minced fish: Evaluation by different methodologies. Food Chem, 101: 372-378.
3. AOAC (1990). Official methods of analysis. 15th Edition, Washington, DC, Association of Official Analytical Chemists.
4. AOCS (American Oil Chemists’ Society) (1998). AOCS Official Method Cd 19-90. 2-Thiobarbituric acid value. Direct Method. In: Official Methods and Recommended Practices of the American Oil Chemists’ Society, Firestone, D. (Ed.), AOCS, Champaign, III.
5. Bozkurt, A.F. (2010). Farklı düzeylerde propolis uygulamalarının farelerde lipid peroksidasyonu (MDA) ile bazı biyokimyasal parametrelere etkilerinin değerlendirilmesi. Yüksek Lisans Tezi, Selçuk Üniversitesi Sağlık Bilimleri Enstitüsü, Konya.
6. Hamilton, R.H., Kalu, C., McNeill, G.P., Padley, F.B., Pierce, J.H. (1998). Effects of tocopherols, ascorbyl palmitate, and lecithin on autoxidation of fish oil. J Am Oil Chem Soc, 75(7): 813-822.
7. Hsieh, R.J., Kinsella, J.E. (1989). Oxidation of polyunsaturated fatty acids: mechanisms, products. Adv Food Nutr Res, 33: 233-341.
8. Iqbal, S., Bhanger, M.I. (2007). Stabilization of sunflower oil by garlic extract during accelerated storage. Food Chem, 100: 246-254.

9. Iqbal, S., Haleem, S., Akhtar, M., Zia-ul-Haq, M., Akbar, J. (2008). Efficiency of pomegranate peel extracts in stabilization of sunflower oil under accelerated conditions. Food Res Int, 41: 194-200.
10. IUPAC (International Union of Pure and Applied Chemistry) (1987a). Method Number 2.505., Evidence of purity and detoriation from ultraviolet spectrometry. In: Standard Methods for the Analysis of Oils, Fats and Derivates, Paquet, C., Hautfenne, A. (Eds), Oxford, UK: Blackwell Scientific Publications, pp: 144-145.
11. IUPAC (International Union of Pure and Applied Chemistry) (1987b). Method Number 2.504. Determination of the p-anisidine value (P-Av). In: Standard Methods for the Analysis of Oils. Fats and Derivates, Paquet, C., , Hautfenne, A. (Eds), Oxford. UK, Blackwell Scientific Publications, pp. 143-144.
12. Kanner, J., Rosenthal, I. (1992). An assessment of lipid oxidation in foods. Pure Appl Chem, 64(12): 1959-1964.
13. Kolanowski, W., Jaworska, D., Weissbrodt, J. (2007). Importance of instrumental and sensory analysis in the assessment of oxidative deterioriation of omega-3 long-chain polyunsaturated fatty acid-rich foods. J Sci Food Agric, 87: 181-191.
14. Nakajima, Y., Shimazawa, M., Mishima, S., Hara, H. (2007). Water extract of propolis and its main constituents, caffeoylquinic acid derivatives, exert neuroprotective effects via antioxidant actions. Life Sci, 80: 370-377.
15. Pietta, P.G., Gardana, C., Pietta, A.M. (2002). Analytical methods quality control of propolis. Fitoterapia, 73: 7-20.
16. Shahidi, F. (2015). Omega-3 fatty acids and marine oils in cardiovascular and general health: A critical overview of controversies and realities. J Funct Foods, 19: 797-800.
17. Shahidi, F., Wanasundara, U.N. (1998). Methods of measuring oxidative rancidity in fats and oils. In: Akoh, C.C., Min, D.B. (Eds.), Food lipids, Chemistry, Nutrition, and Biotechnology, CRC Press Inc. Boca Raton, Florida, pp. 377–396).
18. Shaidi, F., Wanasundara, U.N. (2002). Methods for measuring oxidative rancidity in fats and oils. In: Food Lipids. Marcel Dekker, New York, USA, pp. 465-485.
19. Tabaraki, R., Heidarizadi, E., Benvidi, A. (2012). Optimization of ultrasonicassisted extraction of pomegranate (Punica granatum L.) peel antioxidants by response surface methodology. Sep Purif Technol, 98: 16-23.
20. Topuz, O.K., Yerlikaya, P., Ucak, I., Gumus, B., Buyukbenli, H.A., Gokoglu, N. (2015). Influence of pomegranate peel (Punica granatum) extract on lipid oxidation in anchovy fish oil under heat accelerated conditions. J Food Sci Technol, 52(1): 625-632.
21. Viuda-Martos, M., Ruiz-Navajas, Y., Fernandez-Lopez, J., Perez-Alvarez, J.A. (2008). Functional properties of Honey, Propolis, and Royal Jelly. J Food Sci, 73: 117-124.
22. Yanishlieva, N.V., Marinova, E.M. (2001). Stabilisation of edible oils with natural antioxidants. Eur J Lipid Sci Technol, 103: 752-767.