FARKLI YUMURTALARIN VİTAMİN, PEPTİD VE MALONDİALDEHİT İÇERİĞİNİN KARŞILAŞTIRILMASI

Abstract

Bu çalışmada, köy tavuğu (organik), çiftlik tavuğu, ördek, bıldırcın ve kaz yumurtalarındaki A, E, C vitaminleri, malondialdehit (MDA), beta-karoten, likopen, glutatyon ve grelin miktarları HPLC ile belirlenmiştir. Çiftlik tavuğu yumurtasındaki A ve E vitamini miktarlarının daha yüksek β-karoten ve likopen miktarlarının ise organik tavuk yumurtasında daha yüksek (P<0.005) olduğu belirlenmiş. Diğer taraftan C vitamini, grelin, GSSG ve MDA bıldırcın yumurtasında daha yüksek, fakat GSH miktarının ise gaz yumurtasında daha fazla olduğu belirlendi. Bulgularımız, organik tavuk yumurtasındaki A vitamini ve GSSG miktarının daha düşük (P<0.005) olduğunu, E vitamini miktarının ise bıldırcın yumurtasında daha düşük olduğunu göstermektedir. Kaz yumurtasında C vitamini, β-karoten ve MDA'nın daha düşük olduğu belirlendi. Diğer taraftan, likopen ve GSH'nin ördek yumurtasında daha düşük iken, çiftlik tavuğu yumurtasındaki grelin miktarı daha düşük (P<0.005) bulunmuştur. Sonuç olarak bıldırcın yumurtasındaki MDA ve GSSG değerlerinin diğer yumurtalara göre daha yüksek olması, bıldırcınların yaşadığı ortamın kuşlar üzerinde stres oluşturması ile açıklanabilir. 

Keywords

• Yumurta,vitamin,β-karoten,likopen,grelin,malondialdehit,glutatyon

COMPARISON OF VITAMIN, PEPTIDE AND MALONDIALDEHYDE CONTENT OF DIFFERENT EGGS

Abstract

In this work, vitamin A, E, C, MDA, beta-carotene, lycopene, glutathione and ghrelin levels of local chicken (organic), farm chicken, duck, quail and goose eggs were determined by HPLC. The amount of vitamin A and E found to be higher in the farm chicken egg than other eggs. β-carotene and lycopene found to be the higher (P<0.005) in the organic chicken egg. On the other hand, vitamin C, ghrelin, GSSG and MDA found to be higher in the quail's egg, but GSH was the higher in goose egg. Vitamin A and GSSG were lowest in the organic chicken egg while vitamin E was the lowest (P<0.005) in the quail's egg. The amount of vitamin C and β-carotene and MDA were found to be lowest in goose egg. Also, lycopene and GSH were found to be lowest in the duck egg, but ghrelin was found lowest (P<0.005) in the farm chicken egg. 

Keywords

• Egg,β-carotene,lycopene,ghrelin,malondialdehyde,vitamin,glutathione

References

1. Agarwal. A., Prahakaran. S. A. and Said.T.M. (2005). Strategies to prevent oxidative stress induced injury to human spermatozoa. Review Article. J Androl, 26: 653-660.
2. Akdemir. F.. Orhan. C.. Sahin. N.. Sahin. K.. and Hayırlı. A. (2012). Tomato powder in laying hen diets: effects on concentrations of yolk carotenoids and lipid peroxidation. Br Poult Sci, 53: 675-680.
3. Applegate. E. (2000). Introduction. nutritional and functional roles of eggs in the diet. J Am Coll Nutr, 19: 495-498.
4. Aydin. S.. Karatas. F. and Geckil H. (2008). Simultaneous quantification of acylated and desacylated ghrelin in biological fluids. Biomed Chromatogr, 22: 1354-1359.
5. Bakalivanov. S.. Tsvetkova. E.. Bakalivanova. T.. Tsvetkov. T.. Kaloyanov. N.. Grigorova. S.. Alexieva. V. (2008). Characterization of freeze–dried egg melange long stored after irradiation. Radiat Phys Chem, 77: 58–63.
6. Barboza. P.S., Parker. K.L. and Hume. I.D. (2009). Metabolic constituents: water. minerals and vitamins. Integrative wildlife nutrition book II. Germany: Springer-Verlag Berlin and Heidelberg GmbH & Co. K; pp. 157–206.
7. Catignani. G. L. (1983). Simultancous determination of retinol and α-tocopherol in serum or plazma by liquid chromatography. Clin Chem, 2914: 708-712.
8. Cerhata. D.. Bauerova. A. and Ginter. E. (1994). Determination of Ascorbic Acid in blood serum using high-performance liquid chromatography and its correlation with spectrophotometric. Caska-Slov-Farm, 43(4): 166-168.

9. Chai. Y. C.. Ashraf. S. S.. Rokutan. K.. Johnston. R.B.. Jr. Thomas. J.A. (1994). Sthiolation of individual human neutrophil proteins including actin by stimulation of the respiratory burst: evidence againist a role for glutathione disulfide. Arch Biochem Biophys, 310: 273-281.
10. Cheesman. K. H., Slater. T. F. (1993). Introduction to free radical biochemistryBr Med Bull, 49: 481-493.
11. Dawes. P.. Dawes. E. (2000). SGE Choromatography Products Catalog. pg: 182.
12. Emilia, N., Chukwuemeka, M. S., Emmanuel C, D., Johnkennedy, N., Pricilla, E. (2016). Quail eggs consumption and the levels of selected mineral in healthy students of Nnamdi Azikiwe University, Nnewi. Int J Pharmacol Therapeutics, 6(4): 1-5. eISSN 2249 – 6467.
13. Fang. Y.Z.. Yang. S. and Wu. G. (2002). Free radicals. antioxidants. and nutrition. Nutrition, 18: 872-879.
14. Gutierrez M. A., Takahashi H.. Juneja L.R.. (1996). Nutritive evaluation of hen eggs In: Hen eggs their basic and applied science. Eds T. Yamamoto. L.R. Juneja. H. Hatta. M. Kim. CRC Press Boca Raton. 25-35.
15. Hansen P.. Scoble J.A., Hanson B.. Hoogenraad N. J. (1998). Isolation and purification of immunoglobulins from chicken eggs using thiophilic interaction chromatography. J Immunol Methods, 215: 1-7.
16. Hargitai, R., Nyiri, Z., Eke, Z., Török, J. (2016). Effects of Temperature and Duration of Storage on the Stability of Antioxidant Compounds in Egg Yolk and Plasma. . Physiol Biochem Zool, 89(2): 161–167
17. Hencken. H.. (1992): Chemical and physiological behavior of feed caratenoids and their effects on pigmentation. Poult Sci, 71: 711-717.
18. Huang. W. Y., Majumder. K., Wu. J. (2010). Oxygen radical absorbance capacity of peptides from egg white protein ovotransferrin and their interaction with phytochemicals. Food Chem, 123: 635–641.
19. Irie. T.. Sugimoto. T.. Ueki. N.. Senoo. H.. Seki. T. (2010). Retinoid storage in the egg of reptiles and birds. Comp Biochem Physiol. Part B 157: 113–118.
20. Islam, K.M.S., Schweigert, F.J. (2015). Comparison of three spectrophotometric methods for analysis of egg yolk carotenoids. Food Chem, 172: 233–237
21. Jung-Woo. K. (2008). Effects of lycopene as a Feed Additive on the Quality of Chicken eggs lycopene. Korean J Poult Sci, 35(3): 275-281.
22. Karadas. F.. Grammendis. E.. Surai. P. F. (2006). Effects of carotenoids from lucerne. marigold and tomato on egg yolk pigmentation and carotenoid composition. Br Poult Sci, 47: 561-566.
23. Karatas. F.. Karatepe. M.. Baysar A.. (2002). Determination of free malondialdehyde in human serum by high performance liquid chromatography. Anal Biochem, 311: 76-79.
24. Karatas. F.. Öbek. E.. Kamışlı F. (2009). Antioxidant capacity of Lemna gibba L. Exposed to wastewater treatment. J Ecol Eng, 35: 1225–1230.
25. Kerver. J.M., Park. Y. and Song. W.O. (2002). The role of eggs in American diets: health implications and benefits. In: Watson R. editor. Eggs and health promotion. Iowa: Blackwell Publishing Co. pp. 9-18.
26. Kocsy. G.. Galiba. G.. Brunold. C. (2001). Role of glutathione in adaptation and signalling during chilling and cold acclimation in plants. Physiol Plantarum, 113: 158–164.
27. Ma. X.. Lin. Y.. Zhang. H.. Chen. W.. Wang. S.. Ruan. D.. Jiang. Z. (2014). Heat stress impairs the nutritional metabolism and reduces the productivity of egg-laying ducks. Anim Reprod Sci, 145: 182–190.
28. Marques, R. H., Gravena, R., A., Torre da Silva, J. D., Roccon, J., Picarelli, J., Hada, F. H., Sandra Aidar de Queiroz, S. A., Moraes, V. M. B. (2011). Effect of supplementation of diets for quails with vitamins A, D and E on performance of the birds and quality and enrichment of eggs. Rev Bras Zootecn, 40(6): 1222-1232
29. McGraw. K.J.. Adkins-Regan. E.and Parker. R.S. (2005). Maternally derived carotenoid pigments affect offspring survival. sex ratio. and sexual attractiveness in a colorful songbird. Naturwissenschaften, 92: 375-380.
30. Miller. K. W.. Lorr. N. A.. Yang. C. S. (1984). Simultaneous determination of plazma retinol α-tocoferol. lycopene. α-carotene. and β-carotene by high performance liquid chromatography. Anal Biochem, 138: 340-345.
31. Morel. Y.. Barouki. R. (1999). Repression of gene expression by oxidative stress. Biochem J, 342: 481–496.
32. Murcia. M.A.. Martı´nez-Tome´ M.. del Cerro. I.. Sotillo. F.. and Ramı´rez. A. (1999). Proximate composition and vitamin E levels in egg yolk: losses by cooking in a microwave oven. J Sci Food Agric, 79: 1550-1556.
33. Nimalaratne. C.. Lopes-Lutz. D.. Schieber. A.. Wu. J. (2011). Free aromatic amino acids in egg yolk show antioxidant properties. Food Chem, 129: 155–161.
34. Nowaczewski, S., Kontecka, H., Krystianiak, S. (2012). Effect of in ovo Injection of vitamin C during incubation on hatchability of chickens and ducks. Folia Biol (Krakow), 60(1-2): 93-97
35. Rotolo, L., Strazzullo, G., Pagella, M., . Brugiapaglia, A., Pozzo, L Schiavone, A. (2010). Effect of a tomato extract-supplemented diet on egg yolk pigmentation and lycopene transfer effıciency. Italian J Food Sci, 22(2): 180-185.
36. Rubolini. D.. Romano. M.. Bonisoli Alquati. A. and Saino. N. (2006). Early maternal. genetic and environmental components of antioxidant protection. morphology and immunity of Yellow-legged Gull (Larus michahellis) chicks. J Evol Biol, 19: 1571-1584.
37. Sakanaka S., Kitahata K., Mitsuya T.. Gutierrez M. A. (2000). Protein quality determination of delipidated egg-yolk. J Food Compost Anal, 13: 773-781.
38. Sies. H.; Stahl. W. (1995). Vitamins E and C. β-carotene. and other carotenoids as antioxidants. Am J Clin Nutr, 62: 1315–1321.
39. Sigma-Aldrich Chemie GmbH.. Supelco Chromatography Products for Analysis & Purification. Export Department Eschenstraße Taufkirchen. 131s. 169s. Germany. 2005-2006.
40. Surai. P. F.. Steinberg. W.. Wakeman. W. G.. Speake. B.K. and Sparks. N.H.C. (2003). Effect of canthaxanthin content of the maternal diet on the antioxidant system of the developing chick. Br Poult Sci, 44: 612–619.
41. Tunsaringkarn, T., Tungjaroenchai, W., Siriwong, W. (2013). Nutrient Benefits of quail (Coturnix Coturnix Japonica) eggs. IJSRP, 3(5): 1-8
42. Yoshimura. Y.. Tsuyuki. C.. Subedi. K.. Kaiya. H.. Sugino. T.. Isobe. N. (2009). Identification of ghrelin in fertilized eggs og chicken. J Poult Sci, 46: 257-259.