DETECTION OF GENETICALLY MODIFIED MAIZE IN FOODS AND FEEDSTUFF BY PCR METHODS

Abstract

In this study twenty six maize kernels and 64 processed maize food including maize flour, starch, corn flakes were collected from different markets located in Turkey, analyzed for genetic modification using the polymerase chain reaction. The samples were examined for the presence of genetic elements located in the majority of transgenic crops such as NOS terminator and CaMV 35S promoter using conventional PCR and then verified real time PCR, too. Additionally, Bt11, Bt176, Mon810, CBH351 and T25 events which have been enjoyed, limited data in Turkey, examined in the products using conventional PCR. Then, quantification of the all lines (except CBH351) was performed via real time PCR too. The results indicated that foreign genetic elements were found in analyzed 14 samples raw and processed materials and the quantity in one sample (maize flour) was greater than 0.9%, the limit at which labeling is required by Biosafety Law in Turkey.

Keywords

• GM maize (corn),feedstuffs,PCR,real-time PCR

GIDA VE YEMLERDE GENETİK MODİFİYE MISIRIN PCR METOTLARI İLE TESPİTİ

Abstract

Bu çalışmada Türkiye piyasasından toplanan ve mısır unu, mısır nişastası, mısır cipsi ve mısır gevreğini içeren 64 adet işlenmiş mısır ürünü ile 19 adet işlenmemiş yemlik tane mısırda PCR yöntemi ile genetik modifikasyon araştırılmıştır. Tüm numunelerde, birçok bitkide düzenleyici elementlerden olan 35S promotör ve NOS terminatör taranması önce konvensiyonel PCR ile yapılarak daha sonra real time PCR ile doğrulanmıştır. Ürünlerde, Türkiye’ de kısıtlı veriye sahip olan Bt11, Bt176, Mon810, T25 ve CBH351 mısırların varlığı konvensiyonel PCR ile aranmış ve CBH351 hariç adı geçen tüm hatlarda real-time PCR kullanılarak miktar analizi yapılmıştır.  Sonuçlar 14 örnekte yabancı genetik element bulunduğunu, bir örneğin (mısır unu) ise Türkiye “Biyogüvenlik Yasası” na göre %0.9 olan etiketleme değerinin üstünde olduğunu göstermektedir. 

Keywords

• GM mısır,yem,konvensiyonel PCR,real-time PCR

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