KASTAMONU SARIMSAĞINDAN MİKRODALGA DESTEKLİ CLEVENGER SİSTEMİYLE ELDE EDİLEN YAĞIN UÇUCU BİLEŞENLERİNİN VE ANTİOKSİDAN AKTİVİTESİNİN BELİRLENMESİ

Abstract

Bu çalışmada, mikrodalga destekli clevenger sistemiyle, geleneksel yöntemlere kıyasla daha kısa sürede çözücüsüz olarak elde edilmiş, Allium sativum (sarımsak, Kastamonu bölgesinden toplanmış) uçucu yağında gaz kromatografisi/kütle spektroskopisi (GC/MS) yardımıyla on dört bileşen tespit edilmiştir. Uçucu yağ örneğinde; alil trisülfit (27.09%), allil metil trisülfit (21.26%), alil disülfit (20.02%), allil trans-1-propenil disülfit (7.48%), allil metil disülfit (6.08%) ve 2-vinil-4H-1,3-dithiin (4.32%) en fazla bulunan sülfür bileşikleridir. Ayrıca, uçucu yağın toplam fenolik madde içeriği 2.84 mg gallik asit eşdeğeri/g ve 2,2-difenil-1-pikrilhydrazil (DPPH) çözeltisinin %50’sini indirgemek için gerekli olan miktarın bir ifadesi ve antioksidan aktivitenin bir indikatörü olan IC₅₀ değeri ise 63.58 g/L olarak hesaplanmıştır. Bütillendirilmiş hidroksianisol (BHA) ve 6-Hidroksi-2,5,7,8-tetrametilkroman-2-karbosilik asit (troloks) çalışmada referans olarak kabul edilmiş sentetik antioksidanlardır ve IC₅₀ değerleri sırasıyla 0.09 ve 0.08 mg/L olarak bulunmuştur.

Keywords

• Allium sativum,sarımsak,uçucu bileşen,fenolikler,antioksidan aktivite

DETERMINATION OF VOLATILE COMPONENTS AND ANTIOXIDANT ACTIVITY OF ESSENTIAL OIL OBTAINED FROM KASTAMONU GARLIC BY MICROWAVE-ASSISTED CLEVENGER SYSTEM

Abstract

In present study, fourteen compounds in     Allium sativum (garlic, harvested from Kastamonu province) essential oil which was obtained by using a microwave-assisted clevenger system with a solvent-free option in a shorter time than traditional methods were identified by gas chromatography/mass spectrometry (GC/MS). Allyl trisulfide (27.09%), allyl methyl trisulfide (21.26%), allyl disulfide (20.02%), allyl trans-1-propenyl disulfide (7.48%), allyl methyl disulfide (6.08%), 2-vinyl-4H-1,3-dithiine (4.32%) were the most abundant sulphur compounds in oil. Also, total phenolic content was calculated as 2.84 mg gallic acid equivalent (GAE)/g and IC₅₀ value which defined the required amount to reduce the 50% of 2,2-Diphenyl-1-picrylhydrazyl (DPPH) solution and an indicator for antioxidant activity was 63.58 g/L. Butylated hydroxyanisole (BHA) and 6-Hydroxy-2,5,7,8-tetramethylchromane-2-carboxylic acid (trolox) were accepted as reference synthetic antioxidants and their IC₅₀ values were recorded as 0.09 and 0.08 g/L respectively. 

Keywords

• Allium sativum,garlic,volatile compound,phenolics,antioxidant activity

References

1. Abu-Lafi,, S., Dembicki, JW., Goldshlag, P., Hanuš, L.O., Dembitsky, V.M. (2004). The use of the ‘cryogenic’ GC/MS and on-column injection for study of organosulfur compounds of the Allium sativum. J Food Comp Anal, 17: 235-245, doi: 10.1016/j.jfca.2003.09.002
2. Adams, R. P. (2001). Identification of essential oil components by gas chromatography/quadrupole mass spectroscopy. Allured Publishing Corporation, Carol Stream, IL, doi: 10.1016/j.jasms.2005.07.008
3. Albi, T., Lanzón, A., Guinda, A., León, M., Pérez-Camino, M. C. (1997). Microwave and conventional heating effects on thermoxidative degradation of edible fats. J Agric Food Chem, 45(10): 3795–3798, doi: 10.1021/jf970181x
4. Arts, I. C. W., Hollman, P. C. H (2005). Polyphenols and disease risk in epidemiologic studies. Am J Clin Nutr, 81: 317S–325S, doi: 10.1093/ajcn/81.1.317S
5. Asdaq, S. M., Inamdar, M. N. (2010). Potential of garlic and its active constituent, S-allyl cystein, as antihypertensive and cardioprotective in presence of captopril. Phytomedicine, 17: 1016-1026, doi: 10.1016/j.phymed.2010.07.012
6. Avato, P., Miccolis, V., Tursi, F. (1998). Agronomic evaluation and essential oil content of garlic (Allium sativum L.) ecotypes grown in Southern Italy. Adv Hort Sci, 12: 201-204.
7. Benkeblia, N. (2004). Antimicrobial activity of essential oil extracts of various onions (Allium cepa) and garlic (Allium sativum). LWT-Food Sci Technol, 37(2): 263-268, doi: 10.1016/j.lwt.2003.09.001
8. Casella, S., Leonardi, M., Melai, B., Fratini, F., Pistelli, L. (2013). The role of diallyl sulfides and dipropyl sulfides in the in vitro antimicrobial activity of the essential oil of garlic, Allium sativum L., and leek, Allium porrum L. Phytother Res, 27(3): 380-383, doi: 10.1002/ptr.4725

9. Corzo-Martinez, M., Corzo, N., Villamiel, M. (2007). Biological properties of onions and garlic. Trends Food Sci Technol, 18: 609-625, doi: 10.1016/j.tifs.2007.07.011
10. Dziri, S., Casabianca, H., Hanchi, B., Hosni, K. (2014). Composition of garlic essential oil (Allium sativum L.) as influenced by drying method. J Essent Oil Res, 26(2): 91-96, doi: 10.1080/10412905.2013.868329
11. Djilani, A., Dicko, A. (2012). The therapeutic benefits of essential oils. In: Bouayed, J., Bohn, T. (Eds.), Nutrition,Well-being and Health. In Tech, Croatia, 155-178, doi: 10.5772/25344
12. El-Sayed, H. S., Chizzola, R., Ramadan, A. A., Edris, A. E. (2017). Chemical composition and antimicrobial activity of garlic essential oils evaluated in organic solvent, emulsifying, and self-microemulsifying water based delivery systems. Food Chem, 221: 196-204, doi: 10.1016/j.foodchem.2016.10.052
13. Fenwick, G., Hanley, A. (1985). The genus Allium. Part 2. Crit Rev Food Sci Nutr, 22(4): 273-377, doi: 10.1080/10408398509527417
14. Filly, A., Fernandez, X., Minuti, M., Visinoni, F., Cravott, G., Chemat, F. (2014). Solvent-free microwave extraction of essential oil from aromatic herbs: From laboratory to pilot and industrial scale. Food Chem, 150: 193-198, doi: 10.1016/j.foodchem.2013.10.139
15. Hussain, A. I., Anwar, F., Hussain Sherazi, S. T., Przybylski, R. (2008). Chemical composition, antioxidant and antimicrobial activities of basil (Ocimum basilicum) essential oils depends on seasonal variations. Food Chem, 108: 986-995, doi: 10.1016/j.foodchem.2007.12.010
16. Jang, H. J., Lee, H. J., Yoon, D. K., Ji, D. S., Kim, J. H., Lee, C. H (2018). Antioxidant and antimicrobial activities of fresh garlic and aged garlic by-products extracted with different solvents. Food Sci Biotechnol, 27(1): 219–225, doi: 10.1007/s10068-017-0246-4
17. Kimbaris, A. C., Kioulos, E., Koliopoulos, G., Polissious, M. G., Michaelakis, A. (2008). Coactivity of sulfide ingredients: a new perspective of the larvicidal activity of garlic essential oil against mosquitoes. Pest Manag Sci, 65: 249-254, doi: 10.1002/ps.1678
18. Lanzotti, V. (2006). The analysis of onions and garlic. J Chromatogr A, 1112: 3-22, doi: 10.1016/j.chroma.2005.12.016
19. Li, X., Wasila, H., Liu, L., Yuan, T., Gao, Z., Zhao, B., Ahmad. I. (2015). Physicochemical characteristics, polyphenol compositions and antioxidant potential of pomegranate juices from 10 Chinese cultivars and the environmental factors analysis. Food Chem, 175; 575-584, doi: 10.1016/j.foodchem.2014.12.003
20. Manzocco, L., Anese, M., Nicoli, M. C. (1998). Antioxidant properties of tea extracts as affected by processing. LWT-Food Sci Technol, 31(7–8): 694-698, doi: 10.1006/fstl.1998.0491
21. Mnayer, D., Fabiano-Tixier, A. S., Petitcolas, E., Hamieh, T., Nehme, N., Ferrant, C., Fernandez, X., Chemat, F. (2014). Chemical composition, antibacterial and antioxidant activities of six essentials oils from the Alliaceae family. Mol, 19(12): 20034–20053, doi: 10.3390/molecules191220034
22. Périno-Issartier, S., Ginies, C., Cravotto, G., Chemat, F. (2013). A comparison of essential oils obtained from lavandin via different extraction processes: Ultrasound, microwave, turbohydrodistillation, steam and hydrodistillation. J Chromatogr A, 1305: 41-47, doi: 10.1016/j.chroma.2013.07.024
23. Petropoulos, S., Fernandes, Â., Barros, L., Ciric, A., Sokovic, M., Ferreira, I. C. F. R. (2018). Antimicrobial and antioxidant properties of various Greek garlic genotypes. Food Chem, 245(8): 7–12, doi: 10.1016/j.foodchem.2017.10.078
24. Rao, P. P., Nagender, A., Rao, L. J., Rao, D. G. (2007). Studies one the effects of microwave drying and cabinet tray drying on the chemical composition of volatile oils of garlic powder. Eur Food Res Technol, 224: 791-795, doi: 10.1007/s00217-006-0364-3
25. Razavi Rohani, S. M., Moradi, M., Mehdizadeh, T., Saei-Dehkordi, S. S., Griffiths, M.W. (2011). The effect of nisin and garlic (Allium sativum L.) essential oil separately and in combination on the growth of Listeria monocytogenes. LWT-Food Sci Technol, 44(10): 2260-2265, doi: 10.1016/j.lwt.2011.07.020
26. Rivlin, R. (2001). Historical perspective on the use of garlic. J Nutr, 131: 951-955, doi: 10.1093/jn/131.3.951S
27. Romeilah, R. M., Fayed, S. A., Mahmoud, G. I. (2010). Chemical compositions, antiviral and antioxidant activities of seven essential oils. J Appl Sci Res, 6(1): 50-62.
28. Schaneberg, B. T., Khan, I. A. (2002). Comparison of extraction methods for marker compounds in the essential oil of lemon grass by GC. J Agric Food Chem, 50(6): 1345-1349, doi: 10.1021/jf011078h
29. Seydim, A. C., Sarikus, G. (2006). Antimicrobial activity of whey protein based edible films incorporated with oregano, rosemary and garlic essential oils. Food Res Int, 39(5): 639-644, doi: 10.1016/j.foodres.2006.01.013
30. Szychowski, K. A., Rybczyńska-Tkaczyk, K., Gaweł-Bęben, K., Świeca, M., Karaś, M., Jakubczyk, A., Matysiak, M., Binduga, U., Gmiński, J. (2018). Characterization of active compounds of different garlic (Allium sativum L.) cultivars. Pol J Food Nutr Sci, 68(1): 73–81, doi: 10.1515/pjfns-2017-0005
31. Taşkın, H., Baktemur, G., Kurul, M., Büyükalaca, S (2013). Use of tissue culture techniques for producing virus-free plant in garlic and their identification through real-time PCR. Sci World J, doi: 10.1155/2013/781282
32. Teixeira, B., Marques, A., Ramos, C., Neng, N. R., Nogueira, J. M. F., Saraiva, J. A., Nunes, M. L., (2013). Chemical composition and antibacterial and antioxidant properties of commercial essential oils. Ind Crops Prod, 43: 587-595, doi: 10.1016/j.indcrop.2012.07.069
33. TÜİK, (2018). Bitkisel üretim istatistikleri. https://biruni.tuik.gov.tr/medas/?kn=92&locale=tr Accessed 06.08.2018
34. Viuda-Martos, M., Navajas, Y. R., Zapata, E. S., Fernández-López, J., Pérez-Álvarez, J. A. (2010). Antioxidant activity of essential oils of five spice plants widely used in a Mediterranean diet. Flavour Fragr J, 25(1): 13-19, doi: 10.1002/ffj.1951
35. Wu, C. C., Chung, J. G., Tsai, S. J., Yang, J. H., Sheen, L. Y. (2004). Differential effects of allyl sulfides from garlic essential oil on cell cycle regulation in human liver tumor cells. Food Chem Toxicol, 42(12): 1937-1947, doi: 10.1016/j.fct.2004.07.008
36. Yen, H. Y., Lin, Y. C (2017). Green extraction of Cymbopogon citrus essential oil by solar energy. Ind Crops Prod, 108(1): 716-721, doi: 10.1016/j.indcrop.2017.07.039
37. Xiong, F., Dai, C. H., Hou, F. R., Zhu, P. P., He, R. H., Ma, H. L. (2018). Study on the ageing method and antioxidant activity of black garlic residues. Czech J. Food Sci, 36(1): 88–97, doi: 10.17221/420/2016-CJFS