BIOGENIC AMINE PRODUCTION IN HISTIDINE DECARBOXYLASE BROTH BY SELECTED LACTIC ACID BACTERIA STRAINS

Abstract

The purpose of the current study was to investigate the biogenic amine production of lactic acid bacteria (LAB) strains (Lactobacillus pentosus, Lactobacillus plantarum, Pediococcus acidilactici, Lactobacillus paraplantarum, Lactobacillus fermentum, Lactococcus raffinolactis, Pediococcus pentosaceus and Leuconostoc mesenteroides) which in fermented foodstuff. Biogenic amine production of LAB strains was monitored in histidine decarboxylase broth using HPLC method. Significant differences were observed on ammonia (AMN) and biogenic amines accumulation among LAB strains (p<0.05). Although P. acidilactici produced lesser AMN than other LAB, it was observed that all LAB strains produced high amount of AMN. LAB strains produced all amines in amounts between 1.48 and 1187.50 mg/L. Histamine production by LAB was in range from 17.20 mg/L by P. acidilactici to 126.26 mg/L by Lb. fermentum. The lowest tyramine concentration was observed by P. acidilactici with value of 14.66 mg/L whilst the highest tyramine production was found by L. raffinolactis with value of 64.43 mg/L.

Keywords

• biogenic amine,lactic acid bacteria,histamine,tyramine

BAZI LAKTİK ASİT BAKTERİ ÜYELERİNİN HİSTİDİN DEKARBOKSİLAZ SIVISINDA BİYOJEN AMİN ÜRETİMLERİ

Abstract

Bu çalışmada fermente gıda ürünlerinden izole edilen yedi laktik asit bakteri türünün (Lactobacillus pentosus, Lactobacillus plantarum, Pediococcus acidilactici, Lactobacillus paraplantarum, Lactobacillus fermentum, Lactococcus raffinolactis, Pediococcus pentosaceus ve Leuconostoc mesenteroides) biyojen amin üretimi araştırılmıştır. LAB üyelerinin, histidin dekarboksilaz sıvısında biyojen amin üretimleri HPLC yöntemi kullanılarak belirlenmiştir. LAB üyeleri arasında amonyak (AMN) ve biyojen amin üretimi bakımından istatistiksel farklılıklar gözlenmiştir (p<0.05). P. acidilactici diğer LAB’lardan daha az amonyak üretmesine rağmen bütün LAB suşlarının yüksek miktarda biyojen amin ürettiği gözlenmiştir. LAB suşları bütün aminleri 1.48 ila 1187.50 mg/L arasında çeşitli miktarlarda üretmiştir. En düşük histamin üretimi 17.20 mg/L ile P. acidilactici tarafından üretilirken en yüksek histamin üretimi 126.26 mg/L ile Lb. fermentum tarafından gerçekleşmiştir. En düşük tiramin konsantrasyonu 14.66 mg/L ile P. acidilactici bakterisinde belirlenirken en yüksek tiramin üretimi 64.43 mg/L ile L. raffinolactis bakterisinde gözlenmiştir. 

Keywords

• biyojen amin,laktik asit bakterileri,histamin,tiramin

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