ERZURUM’DA SÜPERMARETLERDEN TOPLANAN PEYNİR ÖRNEKLERİNDE BELİRLİ MİKROBİYOLOJİK PARAMETRELERİN BELİRLENMESİ

Abstract

Bu çalışmanın amacı 50 farklı peynir örneğini belirli mikrobiyolojik parametreler açısından değerlendirmektir. Peynirlerde Salmonella spp. ve Listeria spp. varlığı immunomanyetik separasyon ve geleneksel metotlarla araştırılmıştır. 50 örneğin 5’i Listeria spp. açısından pozitif bulunurken, 4’ü Staphylococcus aureus, 2’si şüpheli kabul edilen Salmonella spp. olarak belirlendi. Şüpheli Salmonella spp. izolatları identifikasyon aşamasında Proteus mirabilis olarak tanımlandığından örneklerde Salmonella spp. tespit edilmemiştir. Listeria spp. izolatları Listeria monocytogenes (2/5), Listeria grayi (3/5) olarak tanımlandı. Antibiyotik test sonuçlarına göre, Staphylococcus aureus izolatları tetrasiklin, trimetoprim, gentamisin, kloramfenikol, sulfametoksazol/trimetoprim, siprofloksasin antibiyotiklerine %100 seviyesinde duyarlılık gösterdi. Ayrıca Listeria spp. izolatları kloramfenikol, siprofloksasin ve ampisilin antibiyotiklerine karşı yüksek duyarlılık sergilemiştir.

Keywords

• Peynir
• Salmonella
• Listeria
• immünomanyetik separasyon
• antiyotik direnç

DETERMINATION OF CERTAIN MICROBIOLOGICAL PARAMETERS ON CHEESE SAMPLES COLLECTED FROM SUPERMARKETS IN ERZURUM

Abstract

This study aimed to evaluate 50 different cheese samples in terms of certain microbiological parameters. The presence of Salmonella spp. and Listeria spp. in cheeses were investigated by immunomagnetic separation and conventional methods. Staphylococcus aureus was detected in 4 samples, while 2 samples were accepted suspicious of Salmonella spp. and 5/50 samples were found positive with respect to Listeria spp. The suspicious Salmonella spp. isolates were determined as Proteus mirabilis. Therefore, Salmonella spp. were not determined from the samples. The isolates of Listeria spp. were identified as Listeria monocytogenes (2/5), Listeria grayi (3/5). According to antibiotic test results, Staphylococcus aureus isolates showed susceptibility to tetracycline, trimethoprim, gentamicin, chloramphenicol, sulphamethoxazole/trimethoprim, ciprofloxacin at the level of 100%. Additionally, the Listeria spp. isolates displayed high susceptibility to chloramphenicol, ciprofloxacin, and ampicillin.

Keywords

• Cheese
• Salmonella
• Listeria
• Immunomagnetic separation
• antibiotic resistance

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