Lipid Composition and Molecular Speciation of the Triacylglycerol of the Oil of Picralima Nitida (in English)

Abstract

The seed of Picralima nitida was analyzed for the proximate and mineral composition while its oil was chemically evaluated. The crude protein content was found as 10.20±0.50 % while the carbohydrate content was 74.53±0.50 %. The iodine and saponification values were found as 136.40±1.00 and 198.25±0.30 mg KOH/g, respectively. Potassium (114.00±0.20 ppm) was the dominant mineral in the seed while calcium (91.00±0.20 ppm) was the dominant mineral in the oil. C18:1 (50.65±0.2 %) was the major fatty acid while the neutral lipids (96.30±0.50 %) were the predominant lipid class. The molecular speciation of the triacylglycerol revealed the presence of molecular species with equivalent carbon chain number C38 (20.38±0.1 %) as the most abundant species. Monogalactosylmonoacylglycerol (67.95±0.10 %) and phosphatidyl choline (66.30±0.10 %) were the main glycolipids and phospholipids found in the oil. Sterols and hydrocarbons were identified in the unsaponifiable matter of the oil using GC-MS.

Keywords

• Fatty acids, Lipid classes, mineral, Picralima nitida, triacylglycerol

Picralima Nitida Yağının Lipit Kompozisyonu ve Trigliserit Yapısı (İngilizce)

Abstract

Picralima nitida tohumunun bileşim ve mineral analizleri yapılmış ve elde edilen yağlarının kimyasal bileşimleri araştırılmıştır. Tohumda ham protein % 10.20±0.50 olarak tespit edilirken, karbohidrat içeriği % 74.53±0.50 oranında bulunmuştur. İyot ve sabunlaşma sayıları sırasıyla 136.40±1.00 ve 198.25±0.30 mg KOH/g değerlerindedir. Tohumun temel minerali potasyum iken (114.00±0.20 ppm), yağda temel mineral kalsiyumdur (91.00±0.20 ppm). Yağda nötral lipit % 96.30±0.50 oranında hesaplanırken, temel yağ asidi olaik asittir (% 50.65±0.2). Eşdeğer karbon sayısına göre analiz edilen trigliseritlerde C38 trigliseridi en yüksek orana sahiptir (% 20.38 ±0.1). Yağın temel glikolipidi monogalatozilmonogliserol iken (% 67.95±0.10), temel fosfolipidi ise fosfotidil kolindir (% 66.30±0.10). Sterol ve hidrokarbonlar ise GC-MS kullanılarak belirlenmiştir.

Keywords

• Yağ asitleri, lipit sınıfları, mineral, Picralima nitida, trigliserit

References

1. Schneider M. 2001. Phospholipids for functional food. Eur J Lipid Sci Technol, 103, 98-101.
2. Beare-Rogers JL. 1983. "Trans and positional isomers of common fatty acids." In H.H. Draper (ed.) Advances in Nutritional Research. Vol. 5 Plenum Press, New York, pp. 171-200.
3. Anon A. 1987. Word fats and oil report. J Amer Oil Chem Soc,64, 1058-1085.
4. Kapadia GJ, Angerhofer, CK, Ansa-Asamoah RA. 1993. An antimalarial indolemonoterpene alkaloid of Picralima nitida seeds. Planta Medica, 59, 565-600.
5. Wen-hua, L, Jing-Zhu Z, Guo- hua L. 2005. Impacts of sewage irrigation on heavy metal distribution and contamination in Beijing, China. Environ Int,31, 805-812.
6. AOAC. 1984. Official Methods of Analysis, 14 ed, vol.67.Association of Official Analytical Chemist, Arlington, VA, pp503-515.
7. Akintayo ET, Bayer E. 2002. Characterization and some possible uses of Plukenetia conophora and Adenopus breviflorusseeds and seed oils. Biores Technol, 85, 95-97.
8. AOAC. 1990. Official Methods of Analysis. 15ed. Association of Official Analytical Chemists, Arlington, VA.

9. Oderinde RA, Adewuyi A, Ajayi IA. 2008. Determination of the mineral nutrients, characterization and analysis of the fat-soluble vitamins of Caesalpinia pulcherrima and Albizia lebbeckseed and seed oils. Seed Sci Biotechnol, 2, 74-78.
10. Christie WW. 1982. Lipid analysis, 2ndedn. Pergamoon press. pp107-130.
11. Jacin H, Mishkin AR. 1965. Separation of carbohydrates on borate-impregnated silica gel G plates. J Chromatogr, 18,170-173.
12. Avalli A, Contarini G. 2005. Determination of phospholipids in dairy products by SPE/HPLC/ELSD. J Chromatogr, 1071, 185-190.
13. Esuoso KO, Odetokun SM. 1995. Proximate composition and possible industrial utilization of Blighia Sapida seed and seed oils. L Riv Ital Sostanze Grasse,72, 311-313.
14. Noskov SY, Bernèche S, Roux B. 2004. "Control of ion selectivity in potassium channels by electrostatic and dynamic properties of carbonyl ligands". Nature, 431, (7010), 830-834.