Lipid Composition and Molecular Speciation of the Triacylglycerol of the Oil of Picralima Nitida (in English)

Yıl 2012, Cilt: 37 Sayı: 1, 1 - 7, 01.02.2012

Yemisi A. Adebowale Adewale Adewuyi Kayode O. Adebowale

https://izlik.org/JA44KX37FA

Öz

The seed of Picralima nitida was analyzed for the proximate and mineral composition while its oil was chemically evaluated. The crude protein content was found as 10.20±0.50 % while the carbohydrate content was 74.53±0.50 %. The iodine and saponification values were found as 136.40±1.00 and 198.25±0.30 mg KOH/g, respectively. Potassium (114.00±0.20 ppm) was the dominant mineral in the seed while calcium (91.00±0.20 ppm) was the dominant mineral in the oil. C18:1 (50.65±0.2 %) was the major fatty acid while the neutral lipids (96.30±0.50 %) were the predominant lipid class. The molecular speciation of the triacylglycerol revealed the presence of molecular species with equivalent carbon chain number C38 (20.38±0.1 %) as the most abundant species. Monogalactosylmonoacylglycerol (67.95±0.10 %) and phosphatidyl choline (66.30±0.10 %) were the main glycolipids and phospholipids found in the oil. Sterols and hydrocarbons were identified in the unsaponifiable matter of the oil using GC-MS.

Anahtar Kelimeler

Fatty acids , Lipid classes , mineral , Picralima nitida , triacylglycerol

Picralima Nitida Yağının Lipit Kompozisyonu ve Trigliserit Yapısı (İngilizce)

https://izlik.org/JA44KX37FA

Öz

Picralima nitida tohumunun bileşim ve mineral analizleri yapılmış ve elde edilen yağlarının kimyasal bileşimleri araştırılmıştır. Tohumda ham protein % 10.20±0.50 olarak tespit edilirken, karbohidrat içeriği % 74.53±0.50 oranında bulunmuştur. İyot ve sabunlaşma sayıları sırasıyla 136.40±1.00 ve 198.25±0.30 mg KOH/g değerlerindedir. Tohumun temel minerali potasyum iken (114.00±0.20 ppm), yağda temel mineral kalsiyumdur (91.00±0.20 ppm). Yağda nötral lipit % 96.30±0.50 oranında hesaplanırken, temel yağ asidi olaik asittir (% 50.65±0.2). Eşdeğer karbon sayısına göre analiz edilen trigliseritlerde C38 trigliseridi en yüksek orana sahiptir (% 20.38 ±0.1). Yağın temel glikolipidi monogalatozilmonogliserol iken (% 67.95±0.10), temel fosfolipidi ise fosfotidil kolindir (% 66.30±0.10). Sterol ve hidrokarbonlar ise GC-MS kullanılarak belirlenmiştir.

Anahtar Kelimeler

Yağ asitleri , lipit sınıfları , mineral , Picralima nitida , trigliserit

Kaynakça

• Schneider M. 2001. Phospholipids for functional food. Eur J Lipid Sci Technol, 103, 98-101.
• Beare-Rogers JL. 1983. "Trans and positional isomers of common fatty acids." In H.H. Draper (ed.) Advances in Nutritional Research. Vol. 5 Plenum Press, New York, pp. 171-200.
• Anon A. 1987. Word fats and oil report. J Amer Oil Chem Soc,64, 1058-1085.
• Kapadia GJ, Angerhofer, CK, Ansa-Asamoah RA. 1993. An antimalarial indolemonoterpene alkaloid of Picralima nitida seeds. Planta Medica, 59, 565-600.
• Wen-hua, L, Jing-Zhu Z, Guo- hua L. 2005. Impacts of sewage irrigation on heavy metal distribution and contamination in Beijing, China. Environ Int,31, 805-812.
• AOAC. 1984. Official Methods of Analysis, 14 ed, vol.67.Association of Official Analytical Chemist, Arlington, VA, pp503-515.
• Akintayo ET, Bayer E. 2002. Characterization and some possible uses of Plukenetia conophora and Adenopus breviflorusseeds and seed oils. Biores Technol, 85, 95-97.
• AOAC. 1990. Official Methods of Analysis. 15ed. Association of Official Analytical Chemists, Arlington, VA.
• Oderinde RA, Adewuyi A, Ajayi IA. 2008. Determination of the mineral nutrients, characterization and analysis of the fat-soluble vitamins of Caesalpinia pulcherrima and Albizia lebbeckseed and seed oils. Seed Sci Biotechnol, 2, 74-78.
• Christie WW. 1982. Lipid analysis, 2ndedn. Pergamoon press. pp107-130.
• Jacin H, Mishkin AR. 1965. Separation of carbohydrates on borate-impregnated silica gel G plates. J Chromatogr, 18,170-173.
• Avalli A, Contarini G. 2005. Determination of phospholipids in dairy products by SPE/HPLC/ELSD. J Chromatogr, 1071, 185-190.
• Esuoso KO, Odetokun SM. 1995. Proximate composition and possible industrial utilization of Blighia Sapida seed and seed oils. L Riv Ital Sostanze Grasse,72, 311-313.
• Noskov SY, Bernèche S, Roux B. 2004. "Control of ion selectivity in potassium channels by electrostatic and dynamic properties of carbonyl ligands". Nature, 431, (7010), 830-834.