BIOGENIC AMINE PRODUCTION IN HISTIDINE DECARBOXYLASE BROTH BY SELECTED LACTIC ACID BACTERIA STRAINS

Year 2020, Volume: 45 Issue: 1, 31 - 38, 01.12.2019

Hatice Yazgan

https://doi.org/10.15237/gida.GD19108

Abstract

The purpose of the current study
was to investigate the biogenic amine production of lactic acid bacteria (LAB)
strains (Lactobacillus pentosus, Lactobacillus plantarum, Pediococcus
acidilactici, Lactobacillus paraplantarum, Lactobacillus
fermentum, Lactococcus raffinolactis, Pediococcus pentosaceus and
Leuconostoc mesenteroides) which in fermented foodstuff. Biogenic amine
production of LAB strains was monitored in histidine decarboxylase broth using
HPLC method. Significant differences were observed on ammonia (AMN) and
biogenic amines accumulation among LAB strains (p<0.05). Although P.
acidilactici produced lesser AMN than other LAB, it was observed that all
LAB strains produced high amount of AMN. LAB strains produced all amines in
amounts between 1.48 and 1187.50 mg/L. Histamine production by LAB was in range
from 17.20 mg/L by P. acidilactici to 126.26 mg/L by Lb. fermentum.
The lowest tyramine concentration was observed by P. acidilactici with
value of 14.66 mg/L whilst the highest tyramine production was found by L.
raffinolactis with value of 64.43 mg/L.

Keywords

biogenic amine, lactic acid bacteria, histamine, tyramine

Supporting Institution

Çukurova Üniversitesi, Bireysel Projeler Birimi

Thanks

This research was supported by Cukurova University for Department of Scientific Research Projects. The authors thank the Department of Scientific Research Projects at Cukurova University for supporting this research.

BAZI LAKTİK ASİT BAKTERİ ÜYELERİNİN HİSTİDİN DEKARBOKSİLAZ SIVISINDA BİYOJEN AMİN ÜRETİMLERİ

Abstract

Bu çalışmada fermente gıda
ürünlerinden izole edilen yedi laktik asit bakteri türünün (Lactobacillus pentosus,
Lactobacillus plantarum, Pediococcus acidilactici, Lactobacillus
paraplantarum, Lactobacillus fermentum, Lactococcus raffinolactis,
Pediococcus pentosaceus ve Leuconostoc mesenteroides) biyojen
amin üretimi araştırılmıştır. LAB üyelerinin, histidin dekarboksilaz sıvısında
biyojen amin üretimleri HPLC yöntemi kullanılarak belirlenmiştir. LAB üyeleri
arasında amonyak (AMN) ve biyojen amin üretimi bakımından istatistiksel
farklılıklar gözlenmiştir (p<0.05). P. acidilactici diğer LAB’lardan
daha az amonyak üretmesine rağmen bütün LAB suşlarının yüksek miktarda biyojen
amin ürettiği gözlenmiştir. LAB suşları bütün aminleri 1.48 ila 1187.50 mg/L
arasında çeşitli miktarlarda üretmiştir. En düşük histamin üretimi 17.20 mg/L
ile P. acidilactici tarafından üretilirken en yüksek histamin üretimi
126.26 mg/L ile Lb. fermentum tarafından gerçekleşmiştir. En düşük
tiramin konsantrasyonu 14.66 mg/L ile P. acidilactici bakterisinde
belirlenirken en yüksek tiramin üretimi 64.43 mg/L ile L. raffinolactis
bakterisinde gözlenmiştir. 

Keywords

biyojen amin, laktik asit bakterileri, histamin, tiramin

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