Investigation of antioxidant activity, total phenolic content and -glucosidase enzyme inhibition potential of Trachystemon orientalis (L.) G. Don plant

Year 2024, Issue: 32, 27 - 36

Fatma Cebeci Tayyibe Erten

Abstract

Objective: This study aims to determine the protein content, antioxidant activity, total phenolic content, and α-glucosidase inhibition rate of the parts of Trachystemon orientalis (L.) G. Don plant separating the plant into 3 parts: leaf, stem, and rhizome.
Material and Method: Proximate analyses of the plant were made according to the methods of the Association of Official Analytical Chemists (AOAC). Spectrophotometric methods were used to determine antioxidant activity, total phenolic content analysis, and α-glucosidase inhibition. One-way ANOVA and Tukey tests were applied to evaluate the differences between samples (p<0.05).
Results and Conclusion: The ash and dry matter ratios of the leaf, stem, and rhizome parts of the plant were found as statistically significant. While the highest protein content was obtained from the leaf (15.67%), no significant difference was observed between the stem and rhizome. The highest antioxidant activity was observed in the rhizome, followed by the leaf and stem, respectively (p<0.05) for DPPH and ABTS methods. The results of the total phenolic content of leaf, stem, and rhizome were compatible with the antioxidant activity results, and the total phenolic content was found as rhizome>leaf>stem with the values of 2.78 ± 0.04, 1.11 ± 0.06 and 0.81 ± 0.07 mg GAE /g fresh weight, respectively. In addition, the α-glucosidase inhibition values of leaf (52.06 ± 2.87) and rhizome (54.34 ± 1.10) extracts were found to be higher than the standard α-glucosidase inhibitor acarbose (43.05±1.27) (p<0.05). As a result, this study showed that all edible and inedible parts of the Trachystemon orientalis (L.) G. Don plants are important in terms of protein content and it has been revealed that this plant can enhance human health with its antioxidant and antidiabetic properties.

Keywords

Trachystemon orientalis, antioxidant activity, protein, total phenolic content, α-glucosidase

Trachystemon orientalis (L.) G. Don bitkisinin antioksidan aktivite, toplam fenolik madde içeriği ve -glukozidaz enzimi inhibisyon potansiyelinin araştırılması

Abstract

Amaç: Bu çalışmanın amacı Trachystemon orientalis (L.) G.Don bitkisini yaprak, sap ve rizom olarak 3 kısma ayırıp, bu kısımların protein içeriğini, antioksidan aktivitesini, toplam fenolik madde içeriğini ve α- glukozidaz inhibisyon oranını belirlemektir.
Materyal ve Yöntem: Bitkinin proksimet analizleri Association of Official Analytical Chemists’in (AOAC) metotlarına göre yapılmıştır. Antioksidan aktivite, toplam fenolik madde analizleri ve α- glukosidaz inhibisyonunun belirlenmesinde ise spektrofotometrik yöntemler kullanılmıştır. Örnekler arasındaki farklılıkların değerlendirilmesinde tek yönlü ANOVA ve Tukey testi uygulanmıştır (p<0,05).
Tartışma ve Sonuç: Bitkinin yaprak, sap ve rizom kısımlarının içerdiği kül ve kuru madde oranları istatistiksel olarak anlamlı farklılık içermektedir. Buna karşın protein oranı en çok yaprak kısmından elde edilirken (%15,67), sap ve rizom kısımlarında önemli bir fark görülmemiştir. DPPH ve ABTS yöntemleriyle antioksidan aktivitesi ölçüldüğünde; en yüksek antioksidan aktivite rizom kısmında, ardından sırasıyla yaprak ve sap kısımlarında görülmüştür (p<0,05). Yaprak, sap, rizom kısımlarının toplam fenolik madde içeriği, antioksidan aktivite sonuçlarıyla paralellik göstermektedir. Toplam fenolik madde içeriği rizom>yaprak>sap şeklinde, sırasıyla 2,78  0,04; 1,11  0,06 ve 0,81  0,07 mg GAE/ g taze ağırlık olarak bulunmuştur. Yaprak (%52,06  2,87) ve rizom (%54,34  1,10) ekstraktlarının α-glukozidaz inhibisyon değerleri standart α-glukozidaz inhibitörü olan akarboza (%43,051,27) göre daha yüksek bulunmuştur (p<0,05). Sonuç olarak bu çalışma, Trachystemon orientalis (L.) G. Don bitkisinin protein içeriği açısından önemli olduğunu; antioksidan ve antidiyabetik özellikleriyle insan sağlığını destekleyebileceğini ortaya koymuştur.

Keywords

Trachystemon orientalis, antioksidan aktivite, protein, toplam fenolik madde, α- glukozidaz

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