Çevre Dostu Bir Yaklaşım: One-Pot (Tek-Kap) Yeşil Sentez ile AgNP Sentezi, Kitosan Konsantrasyonunun Tanecik Boyutu Üzerine Etkisi ve Antibakteriyel Aktiviteleri

Yıl 2024, Cilt: 5 Sayı: 2, 169 - 180, 28.11.2024

Filiz Kara

Öz

Gümüş nanopartikül (AgNP), hem indirgeyici hem de stabilize edici ajan olarak hareket eden kitosan (CH) kullanılarak one-pot yeşil sentez yöntemi ile otoklavda sentezlenmiştir. Farklı konsantrasyonlarda kullanılan kitosanın (%0,3-0,9 w/v) oluşan AgNP (AgNP-CH0.3¬AgNP-CH0.9) özellikleri üzerine etkisi incelenmiştir. UV-vis spektrumları, AgNP-CH'ler için karakteristik olan 423-432 nm aralığında pik göstermiştir. Fizikokimyasal özellikler taramalı elektron mikroskobu (SEM), X-ışını kırınımı (XRD), zetasizer gibi farklı yöntemler kullanılarak değerlendirilmiştir. Sonuçlar kitosan konsantrasyonun artmasıyla nanopartikül boyutunun azaldığını ve en küçük boyuta sahip örneğin yüzey merkezli kübik (fcc) kristal yapıdaki AgNP-CH-0.9 olduğunu ortaya koyulmuştur. Tüm örnekler Escherichia coli (E. coli) ve Staphylococcus aureus (S. aureus)'a karşı antibakteriyal aktiviteye sahipken en yüksek aktiviteyi AgNP-CH-0.9 sergilemiştir. Nanopartikül boyutu küçüldükçe antibakteriyal etkinliğin arttığı belirlenmiştir. Herhangi bir toksik kimyasalın kullanılmadığı, düşük maliyetli, kolay olan bu yöntem çok geniş kullanım alanına sahip AgNP’lerin eldesi için alternatif bir uygulama sunmaktadır.

Anahtar Kelimeler

Yeşil sentez, AgNP, Kitosan, Antibakteriyal aktivite.

Kaynakça

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