EFFECTS OF THE NEW ANTIFUNGAL THIO HALO-BENZENE DERIVATIVE 2,4-DITHIO-PHENOXY-1-IODO-4-BROMO BENZENE MOLECULE ON RAT LIVER AND KIDNEY TISSUE OXIDATIVE EVENTS

Abstract

Nowadays, fluconazole is a widely used antifungal drug. Besides its similarities with the carbon structure of fluconazole, the newly synthesized 2,4-dithio-phenoxy-1-iodo-4-bromo benzene is also more active than low concentrated fluconazole. During and after the drug use, oxidative events occur in the organism. In this study, effects of previously proved as an antibacterial and antifungal molecule 2,4-dithio-phenoxy-1-iodo-4-bromo benzene (C₁₈H₁₂S₂IBr) on mammal liver tissue and mammal kidney using biochemical methods. 80 male and female Wistar albino rats between the range of 150-200 gr have been used for this study.  Total 16 rats have been used as subjects in this experiment and each group consisted of 8 males and 8 females. Rats have been given fluconazole as well as 2,4-dithio-phenoxy-1-iodo-4-bromo benzene orally, each dissolved in alcohol, once in a week for 4 weeks. All subjects separated into 5 groups: a control group, alcohol control, fluconazole group (0.28 mgr/100 gr) and two different doses of 2,4-dithio-phenoxy-1-iodo-4-bromo benzene (0.112 mg/100 gr and 0.056 mg/100 gr). Liver tissues obtained from subjects using cervical dislocation and preserved at -30^oC until examination. Kidney malondialdehyde (MDA), glutathione (GSH), nitric oxide (NO), vitamin C levels (AA) and myeloperoxidase activities (MPO) studied using spectrophotometric methods. Thio halo-benzene derivative 2,4-dithio-phenoxy-1-iodo-4-bromo benzene reduced female MDA levels -the indicator of lipid peroxidation-, and also decreased neutrophil infiltration. As for male rats, no statistically significant differences have been found on other parameters, excluding GSH levels. Thereby it is concluded that this newly synthesized antifungal compound does not cause any damage on female liver tissue. When the effects of newly synthesized thio halo-benzene derivative 2,4-dithio-phenoxy-1-iodo-4-bromo benzene and fluconazole on the lipid peroxidation indicator MDA compared, it has been found that it increases the capacity of kidney tissue antioxidants and there were significantly low statistics. It is concluded that this newly synthesized antifungal compound does not cause any oxidative damage in male kidney tissues when comparing with fluconazole.

Keywords

• Oxidative stress,Thio halo-benzene derivatives,Fluconazole,Antioxidant,Rat,Kidney

References

1. [7] Koçkaya, E. A., Kılıç, A., Selmanoğlu, G., Akay, M. T., Loğoğlu, E., Öktemer, A., “Evaluation of the Effects of Newly Synthesized 2,4-Dithiophenoxy-1-iodo-4-bromo Benzene That Has Anti-Fungal and Anti-Bacterial Activities on Rats. 6th International Congress of Turkish Society of Toxicology”, 2-5 November, Antalya, Turkey (2006).