EFFECTS OF THE NEW ANTIFUNGAL THIO HALO-BENZENE DERIVATIVE 2,4-DITHIO-PHENOXY-1-IODO-4-BROMO BENZENE MOLECULE ON RAT LIVER AND KIDNEY TISSUE OXIDATIVE EVENTS

Year 2017, Volume: 30 Issue: 3, 237 - 244, 20.09.2017

Elif Loğoğlu , Eda Çınar Avar Şule Coşkun Cevher

Abstract

Nowadays, fluconazole is a
widely used antifungal drug. Besides its similarities with the carbon structure
of fluconazole, the newly synthesized 2,4-dithio-phenoxy-1-iodo-4-bromo
benzene is also more active than
low concentrated fluconazole. During and after the drug use,
oxidative events occur in the organism. In this study, effects of previously proved as an antibacterial
and antifungal molecule 2,4-dithio-phenoxy-1-iodo-4-bromo
benzene (C₁₈H₁₂S₂IBr) on mammal liver
tissue and mammal kidney using biochemical methods. 80 male and
female Wistar albino rats between the range of 150-200 gr have been used for
this study.  Total 16 rats have been used
as subjects in this experiment and each group consisted of 8 males and 8
females. Rats have been given fluconazole as well as 2,4-dithio-phenoxy-1-iodo-4-bromo benzene orally, each dissolved
in alcohol, once in a week for 4 weeks. All subjects separated into 5 groups: a
control group, alcohol control, fluconazole group (0.28 mgr/100 gr) and two
different doses of 2,4-dithio-phenoxy-1-iodo-4-bromo
benzene (0.112 mg/100 gr and 0.056 mg/100 gr). Liver tissues obtained
from subjects using cervical dislocation and preserved at -30^oC
until examination. Kidney malondialdehyde (MDA), glutathione (GSH), nitric
oxide (NO), vitamin C levels (AA) and myeloperoxidase activities (MPO) studied
using spectrophotometric methods. Thio halo-benzene derivative 2,4-dithio-phenoxy-1-iodo-4-bromo benzene
reduced female MDA levels -the indicator of lipid peroxidation-, and also
decreased neutrophil infiltration. As for male rats, no statistically
significant differences have been found on other parameters, excluding GSH
levels. Thereby it is concluded that this newly synthesized antifungal compound
does not cause any damage on female liver tissue. When the effects of newly
synthesized thio halo-benzene derivative 2,4-dithio-phenoxy-1-iodo-4-bromo benzene and fluconazole on the
lipid peroxidation indicator MDA compared, it has been found that it increases
the capacity of kidney tissue antioxidants and there were significantly low
statistics. It is concluded that this newly synthesized antifungal compound
does not cause any oxidative damage in male kidney tissues when comparing with
fluconazole.

Keywords

Oxidative stress, Thio halo-benzene derivatives, Fluconazole, Antioxidant, Rat, Kidney

References

• [7] Koçkaya, E. A., Kılıç, A., Selmanoğlu, G., Akay, M. T., Loğoğlu, E., Öktemer, A., “Evaluation of the Effects of Newly Synthesized 2,4-Dithiophenoxy-1-iodo-4-bromo Benzene That Has Anti-Fungal and Anti-Bacterial Activities on Rats. 6th International Congress of Turkish Society of Toxicology”, 2-5 November, Antalya, Turkey (2006).