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Sublethal Exposure and Toxicity Effect of Propanil on Hematology and Serum Biochemistry in Oreochromis niloticus in a Static Bioassay

Year 2018, Volume: 31 Issue: 4, 1048 - 1062, 01.12.2018

Abstract

Herbicide Propanil is a toxicant that can
adversely affect the health of fish. The present study was conducted to
ascertain the toxicity effect of Propanil on hematology and serum biochemistry
in Oreochromis niloticus juvenile. Fish specimens were exposed to sub-lethal
concentrations (0.0, 0.22, 0.44 and 0.87 mg/L) of Propanil derived from the
96hour Lethal concentration for eight weeks. Fish specimens were sampled for
hematological and biochemical analysis at 2, 6 and 8 week’s duration exposure.
Significant changes and dose dependent decreases in red blood cell, pack cell
volume, hemoglobin values were observed in Propanil exposed fish compared to
the control. Progressive increase in white blood cell count and leukocyte
differentials (neutrophils and lymphocytes) were found in Propanil exposed fish
compared to the control. Significant increases in biochemical parameters
(glucose, protein, triglycerides, glutamic oxalo-acetic transminase and
glutamate pyruvate transaminase) were observed in Propanil exposed fish groups
compared to the control. However, cholesterol levels decreased significantly
except for 8th week Propanil duration exposure. Findings of the present study
revealed that Propanil adversely affected fish health.

References

  • Blaxhall PC, Daisley KW. (2006). Routine haematological methods for use with fish blood.Fish Biol, 5: 771-781.
  • Canli M. (1996). Effects of mercury, chromium, and nickel on glycogen reserves and protein levels in tissues of Cyprinus carpio. Turkish J. Zool. 20:161–168
  • Cole MB, Arnold DE, Watten BJ, Krise WF. (2001). Hematological and physiological responses of brook charr, to untreated and limestone neutralized acid mine drainage. J Fish Biol 59:79–91.
  • Dacie, J.V., & Lewis, S.M. (2011). Practical hematology. 11th edition, New York: Churchill Livingstone, pp 41.
  • Dinis-Oliveira RJ, Duarte JA, Sánchez-Navarro A, Remião F, Bastos ML, Carvalho F. (2008) Paraquat poisonings: mechanisms of lung toxicity, clinical features, and treatment. Crit Rev Toxico,l 38: 13-71.
  • El-Sayed YS, Saad TT, El-Bahr SM. (2007) Acute intoxication of deltamethrin in monosex Nile tilapia, Oreochromis niloticus with special reference to the clinical, biochemical and haematological effects. Environ Toxicol Pharmacol , 24: 212-217.
  • Ensibi C, Pérez-López M, Soler Rodríguez F, Míguez-Santiyán MP, Yahya MN, Hernández-Moreno D. (2013). Effects of deltamethrin on biometric parameters and liver biomarkers in common carp (Cyprinus carpio L.). Environ Toxicol Pharmacol , 36: 384-391.
  • Hesser, E.F. (1960). Method for routine fish haematology prog. Fish cult, 22: 164-171.Iheanacho SC, Ogunji JO, Ogueji EO, Nwuba LA, Nnatuanya IO, Ochang SN, Mbah CE, Usman IB, Haruna M. (2017a). Comparative assessment of ampicillin antibiotic and ginger (Zingiber officinale) effects on growth, haematology and biochemical enzymes of Clarias gariepinus Juvenile. J Pharma Phytochem. 6(3): 761-767.
  • Iheanacho S, Ogueji E, Yaji A, Dada O, Mbah C, Ifejimalu A, Ibrahim BU. (2017b). Effects of herbal plants (Zingiber officinale and Hibiscus sabdariffa) as dietary additives on serum biochemistry and some metabolites in Clarias gariepinus (Burchell, 1822). J. Coast. Life Med. 5(12): 516-520. https://doi.org/10.12980/jclm.5.2017.167 Iheanacho SC, Ikwo TN, Igweze N, Chukwuidha C, Ogueji EO, Onyeneke R. (2018). Effect of different dietary inclusion levels of melon seed (Citrullus lanatus) peel on growth, haematology and histology of Oroechromis niloticus juvenile. Turkish J. Fish. Aquat. Sci., 18(3): 377-384. Doi :10.4194/1303-2712-v18_3_03.
  • Jeney G. (2017). Fish diseases ; Prevention and Control Strategies. Academic press, London wall, 8p.
  • Khalid A. A. ( 2012). Acute toxicity and effects of sublethal malathion exposure on biochemical and hematological parameters of Oreochromis niloticus. Scient. Res. Essays 7:1674–1680.
  • Levesque HM, Moon TW, Campbell PGC, Hontela A. (2002). Seasonal variation in carbohydrate and lipid metabolism of yellow perch (Perca flavescens) chronically exposed to metals in the field. Aquat Toxicol 60:257–267.
  • Li ZH, Zlabek V, Velisek J, Grabic R, Machova J, Kolarova J, et al. (2011). Acute toxicity of carbamazepine to juvenile rainbow trout (Oncorhynchus mykiss): effects on antioxidant responses, hematological parameters and hepatic EROD. Ecotoxicol Environ Saf , 74: 319-327.
  • Muralidharan L. (2012). Hemato-biochemical alternations induced by chronic exposure to fenthion in Cyprinus carpio. Trends in Fish. Res. 1:19–25.
  • Nnadi JU, Dimelu IN, Nwani SI, Madu JC, Atama CI, Attamah GN, Okwor JL, Nwani CD. (2018). Biometric variations and oxidative stress responses in juvenile Clarias gariepinus exposed to termex. Afr. J. Aquat. Sci., https://doi.org/10.2989/16085914.2018.1445615.
  • Nwani CD, Nagpure NS, Kumar R, Kushwaha B, Lakra WS. (2013). DNA damage and oxidative stress modulatory effects of glyphosate-based herbicide in freshwater fish, Channa punctatus. Environ Toxicol Pharmacol, 36: 539-547.
  • Nwani CD, Ekwueme HI, Ejere VC, Onyeke CI, Chukwuka CO, Somdare PO, Nwadinigwe AO. (2015). Physiological effects of paraquat in Juvenile African catfish Clarias gariepinus (Burchell, 1882), J. Coast. Life Med., 3(1):35-43. doi: 10.12980/JCLM.3.2015JCLM-2014-0113.
  • Nwani CD, Somdare PO, Ukonze JA, Ejere VC, Nwadinigwe AO, Nwani JC, Odo GE, and Ugbor ON(2016). Subchronic exposure to fenthion induces haematological changes in liver tissue of African catfish Clarias gariepinus, J. Aquat. Anim. Health, 28 (4): 229-234. http://dx. doi.org/10.1030108997659.2016.1194907.
  • Odo GE, Agwu E, Ivoke N, Ejere VC, Atama CI, Ezea CO, Aguoru GC, Anya BC. (2017). Effect of short term exposure to cyperdicot on behavioural and hematological responses in African catfish Clarias gariepinus, Turkish J. Fish Aquat. Sci. 17:61-70. Doi:10.4194/1303-2712v17_1_08.
  • Ogueji EO, Iheanacho SC, Nwani CD, Mbah CE, Okeke OC, Ibrahim BU. (2017). Toxicity of diazepam on lipid peroxidation, biochemical and oxidative stress indicators on liver and gill tissues of African catfish Clarias gariepinus (Burchell, 1822). Inter. J. Fish. Aquat. Stud. 5(3): 114-123
  • Oladimeji AA, Ogunmeta, RT. (1987). Chronic Toxicity of water-borne lead to Tilapia nilotica (L.). Nigerian J. of Appl. Fish Hydrobiol, 2:19-24.
  • Oner MA, Atli G and Canli M (2008). Changes in serum biochemical parameters of fresh water fish Oreochromis niloticus following prolonged metal (Ag, Cd, Cr, Cu, Zn) exposures. Environmental Toxicol and Chem, 27(2): 360-366.
  • Pandey S, Kumar R, Sharma S, Nagpure NS, Srivastava SK, Verma MS. (2005). Acute toxicity bioassays of mercuric chloride and malathion on airbreathing fish Channa punctatus (Bloch). Ecotoxicol Environ Saf, 61: 114-120.
  • Saravanan M, Kumar KP, Ramesh M. (2011). Haematological and biochemical responses of freshwater teleost fish Cyprinus carpio (Actinopterygii: Cypriniformes) during acute and chronic sublethal exposure to lindane. Pestic Biochem Physiol 100: 206-211.
  • Singh HS, Reddy TV. (1990). Effect of copper sulfate on hematology,blood chemistry, and hepatosomatic index of an Indian catfish, Heteropneustes fossillis (Bloch), and its recovery. Ecotoxicol Environ Saf, 20:30–35.
  • Summers LA. (1980). The bipyridinium herbicides. London: Academic Press.
  • Tilak KS, Veeraiah K, Butchiram MS. (2007). Effect of phenol on hematological components of Indian major carps Catla catla, Labeo rohita, and Cirrhinus mirgala. J. Environ. Biol. 28:177–179.
  • Tortorelli MC, Hernández DA, Rey Vázquez G, Salibián A. (1990). Effects of paraquat on mortality and cardiorespiratory function of catfish fry Plecostomus commersoni. Arch Environ Contam Toxicol , 19: 523-529.
  • Yang J, Chen HC. (2003). Effects of gallium on common carp (Cyprinus carpio): Acute test, serum biochemistry, and erythrocyte morphology. Chemosphere 53:877–882.
  • Zhang, X Z, Xie P, Wang WM, Li DP, Shi ZC. (2008). Plasma biochemical responses of the omnivorous Crucian Carp (Carassius auratus) to crude cyanobacterial extracts. Fish Physiol. Biochem. 34:323–329.
Year 2018, Volume: 31 Issue: 4, 1048 - 1062, 01.12.2018

Abstract

References

  • Blaxhall PC, Daisley KW. (2006). Routine haematological methods for use with fish blood.Fish Biol, 5: 771-781.
  • Canli M. (1996). Effects of mercury, chromium, and nickel on glycogen reserves and protein levels in tissues of Cyprinus carpio. Turkish J. Zool. 20:161–168
  • Cole MB, Arnold DE, Watten BJ, Krise WF. (2001). Hematological and physiological responses of brook charr, to untreated and limestone neutralized acid mine drainage. J Fish Biol 59:79–91.
  • Dacie, J.V., & Lewis, S.M. (2011). Practical hematology. 11th edition, New York: Churchill Livingstone, pp 41.
  • Dinis-Oliveira RJ, Duarte JA, Sánchez-Navarro A, Remião F, Bastos ML, Carvalho F. (2008) Paraquat poisonings: mechanisms of lung toxicity, clinical features, and treatment. Crit Rev Toxico,l 38: 13-71.
  • El-Sayed YS, Saad TT, El-Bahr SM. (2007) Acute intoxication of deltamethrin in monosex Nile tilapia, Oreochromis niloticus with special reference to the clinical, biochemical and haematological effects. Environ Toxicol Pharmacol , 24: 212-217.
  • Ensibi C, Pérez-López M, Soler Rodríguez F, Míguez-Santiyán MP, Yahya MN, Hernández-Moreno D. (2013). Effects of deltamethrin on biometric parameters and liver biomarkers in common carp (Cyprinus carpio L.). Environ Toxicol Pharmacol , 36: 384-391.
  • Hesser, E.F. (1960). Method for routine fish haematology prog. Fish cult, 22: 164-171.Iheanacho SC, Ogunji JO, Ogueji EO, Nwuba LA, Nnatuanya IO, Ochang SN, Mbah CE, Usman IB, Haruna M. (2017a). Comparative assessment of ampicillin antibiotic and ginger (Zingiber officinale) effects on growth, haematology and biochemical enzymes of Clarias gariepinus Juvenile. J Pharma Phytochem. 6(3): 761-767.
  • Iheanacho S, Ogueji E, Yaji A, Dada O, Mbah C, Ifejimalu A, Ibrahim BU. (2017b). Effects of herbal plants (Zingiber officinale and Hibiscus sabdariffa) as dietary additives on serum biochemistry and some metabolites in Clarias gariepinus (Burchell, 1822). J. Coast. Life Med. 5(12): 516-520. https://doi.org/10.12980/jclm.5.2017.167 Iheanacho SC, Ikwo TN, Igweze N, Chukwuidha C, Ogueji EO, Onyeneke R. (2018). Effect of different dietary inclusion levels of melon seed (Citrullus lanatus) peel on growth, haematology and histology of Oroechromis niloticus juvenile. Turkish J. Fish. Aquat. Sci., 18(3): 377-384. Doi :10.4194/1303-2712-v18_3_03.
  • Jeney G. (2017). Fish diseases ; Prevention and Control Strategies. Academic press, London wall, 8p.
  • Khalid A. A. ( 2012). Acute toxicity and effects of sublethal malathion exposure on biochemical and hematological parameters of Oreochromis niloticus. Scient. Res. Essays 7:1674–1680.
  • Levesque HM, Moon TW, Campbell PGC, Hontela A. (2002). Seasonal variation in carbohydrate and lipid metabolism of yellow perch (Perca flavescens) chronically exposed to metals in the field. Aquat Toxicol 60:257–267.
  • Li ZH, Zlabek V, Velisek J, Grabic R, Machova J, Kolarova J, et al. (2011). Acute toxicity of carbamazepine to juvenile rainbow trout (Oncorhynchus mykiss): effects on antioxidant responses, hematological parameters and hepatic EROD. Ecotoxicol Environ Saf , 74: 319-327.
  • Muralidharan L. (2012). Hemato-biochemical alternations induced by chronic exposure to fenthion in Cyprinus carpio. Trends in Fish. Res. 1:19–25.
  • Nnadi JU, Dimelu IN, Nwani SI, Madu JC, Atama CI, Attamah GN, Okwor JL, Nwani CD. (2018). Biometric variations and oxidative stress responses in juvenile Clarias gariepinus exposed to termex. Afr. J. Aquat. Sci., https://doi.org/10.2989/16085914.2018.1445615.
  • Nwani CD, Nagpure NS, Kumar R, Kushwaha B, Lakra WS. (2013). DNA damage and oxidative stress modulatory effects of glyphosate-based herbicide in freshwater fish, Channa punctatus. Environ Toxicol Pharmacol, 36: 539-547.
  • Nwani CD, Ekwueme HI, Ejere VC, Onyeke CI, Chukwuka CO, Somdare PO, Nwadinigwe AO. (2015). Physiological effects of paraquat in Juvenile African catfish Clarias gariepinus (Burchell, 1882), J. Coast. Life Med., 3(1):35-43. doi: 10.12980/JCLM.3.2015JCLM-2014-0113.
  • Nwani CD, Somdare PO, Ukonze JA, Ejere VC, Nwadinigwe AO, Nwani JC, Odo GE, and Ugbor ON(2016). Subchronic exposure to fenthion induces haematological changes in liver tissue of African catfish Clarias gariepinus, J. Aquat. Anim. Health, 28 (4): 229-234. http://dx. doi.org/10.1030108997659.2016.1194907.
  • Odo GE, Agwu E, Ivoke N, Ejere VC, Atama CI, Ezea CO, Aguoru GC, Anya BC. (2017). Effect of short term exposure to cyperdicot on behavioural and hematological responses in African catfish Clarias gariepinus, Turkish J. Fish Aquat. Sci. 17:61-70. Doi:10.4194/1303-2712v17_1_08.
  • Ogueji EO, Iheanacho SC, Nwani CD, Mbah CE, Okeke OC, Ibrahim BU. (2017). Toxicity of diazepam on lipid peroxidation, biochemical and oxidative stress indicators on liver and gill tissues of African catfish Clarias gariepinus (Burchell, 1822). Inter. J. Fish. Aquat. Stud. 5(3): 114-123
  • Oladimeji AA, Ogunmeta, RT. (1987). Chronic Toxicity of water-borne lead to Tilapia nilotica (L.). Nigerian J. of Appl. Fish Hydrobiol, 2:19-24.
  • Oner MA, Atli G and Canli M (2008). Changes in serum biochemical parameters of fresh water fish Oreochromis niloticus following prolonged metal (Ag, Cd, Cr, Cu, Zn) exposures. Environmental Toxicol and Chem, 27(2): 360-366.
  • Pandey S, Kumar R, Sharma S, Nagpure NS, Srivastava SK, Verma MS. (2005). Acute toxicity bioassays of mercuric chloride and malathion on airbreathing fish Channa punctatus (Bloch). Ecotoxicol Environ Saf, 61: 114-120.
  • Saravanan M, Kumar KP, Ramesh M. (2011). Haematological and biochemical responses of freshwater teleost fish Cyprinus carpio (Actinopterygii: Cypriniformes) during acute and chronic sublethal exposure to lindane. Pestic Biochem Physiol 100: 206-211.
  • Singh HS, Reddy TV. (1990). Effect of copper sulfate on hematology,blood chemistry, and hepatosomatic index of an Indian catfish, Heteropneustes fossillis (Bloch), and its recovery. Ecotoxicol Environ Saf, 20:30–35.
  • Summers LA. (1980). The bipyridinium herbicides. London: Academic Press.
  • Tilak KS, Veeraiah K, Butchiram MS. (2007). Effect of phenol on hematological components of Indian major carps Catla catla, Labeo rohita, and Cirrhinus mirgala. J. Environ. Biol. 28:177–179.
  • Tortorelli MC, Hernández DA, Rey Vázquez G, Salibián A. (1990). Effects of paraquat on mortality and cardiorespiratory function of catfish fry Plecostomus commersoni. Arch Environ Contam Toxicol , 19: 523-529.
  • Yang J, Chen HC. (2003). Effects of gallium on common carp (Cyprinus carpio): Acute test, serum biochemistry, and erythrocyte morphology. Chemosphere 53:877–882.
  • Zhang, X Z, Xie P, Wang WM, Li DP, Shi ZC. (2008). Plasma biochemical responses of the omnivorous Crucian Carp (Carassius auratus) to crude cyanobacterial extracts. Fish Physiol. Biochem. 34:323–329.
There are 30 citations in total.

Details

Primary Language English
Subjects Engineering
Journal Section Biology
Authors

Abubakar Yajı This is me

Stanley Iheanacho This is me

Emmanuel Oguejı This is me

Publication Date December 1, 2018
Published in Issue Year 2018 Volume: 31 Issue: 4

Cite

APA Yajı, A., Iheanacho, S., & Oguejı, E. (2018). Sublethal Exposure and Toxicity Effect of Propanil on Hematology and Serum Biochemistry in Oreochromis niloticus in a Static Bioassay. Gazi University Journal of Science, 31(4), 1048-1062.
AMA Yajı A, Iheanacho S, Oguejı E. Sublethal Exposure and Toxicity Effect of Propanil on Hematology and Serum Biochemistry in Oreochromis niloticus in a Static Bioassay. Gazi University Journal of Science. December 2018;31(4):1048-1062.
Chicago Yajı, Abubakar, Stanley Iheanacho, and Emmanuel Oguejı. “Sublethal Exposure and Toxicity Effect of Propanil on Hematology and Serum Biochemistry in Oreochromis Niloticus in a Static Bioassay”. Gazi University Journal of Science 31, no. 4 (December 2018): 1048-62.
EndNote Yajı A, Iheanacho S, Oguejı E (December 1, 2018) Sublethal Exposure and Toxicity Effect of Propanil on Hematology and Serum Biochemistry in Oreochromis niloticus in a Static Bioassay. Gazi University Journal of Science 31 4 1048–1062.
IEEE A. Yajı, S. Iheanacho, and E. Oguejı, “Sublethal Exposure and Toxicity Effect of Propanil on Hematology and Serum Biochemistry in Oreochromis niloticus in a Static Bioassay”, Gazi University Journal of Science, vol. 31, no. 4, pp. 1048–1062, 2018.
ISNAD Yajı, Abubakar et al. “Sublethal Exposure and Toxicity Effect of Propanil on Hematology and Serum Biochemistry in Oreochromis Niloticus in a Static Bioassay”. Gazi University Journal of Science 31/4 (December 2018), 1048-1062.
JAMA Yajı A, Iheanacho S, Oguejı E. Sublethal Exposure and Toxicity Effect of Propanil on Hematology and Serum Biochemistry in Oreochromis niloticus in a Static Bioassay. Gazi University Journal of Science. 2018;31:1048–1062.
MLA Yajı, Abubakar et al. “Sublethal Exposure and Toxicity Effect of Propanil on Hematology and Serum Biochemistry in Oreochromis Niloticus in a Static Bioassay”. Gazi University Journal of Science, vol. 31, no. 4, 2018, pp. 1048-62.
Vancouver Yajı A, Iheanacho S, Oguejı E. Sublethal Exposure and Toxicity Effect of Propanil on Hematology and Serum Biochemistry in Oreochromis niloticus in a Static Bioassay. Gazi University Journal of Science. 2018;31(4):1048-62.