Aslanpençesi (Alchemilla ellenbergiana) Ekstrelerinin Antioksidan ve Antimikrobiyal Özelliklerinin Belirlenmesi

Year 2018, Volume: 8 Issue: 1, 113 - 121, 31.01.2018

Tuba Acet , Kadriye Özcan

https://doi.org/10.17714/gumusfenbil.308430

Cited By: 6

Abstract

Bu çalışmada, Gümüşhane bölgesi halk tıbbında
kullanılan Alchemilla ellenbergiana (aslanpençesi) bitkisinin farklı
polariteye sahip çözücülerle (hekzan, etil asetat, etanol ve metanol)
hazırlanmış ekstrelerinin toplam fenolik miktarları, antioksidan ve antimikrobiyal
aktiviteleri araştırıldı. Toplam fenolik miktarı gallik asit eşdeğeri olarak
spektrofotometrik yöntemle ölçüldü ve en yüksek değerler metanol ve etanol
ekstrelerinde tespit edildi. Ekstrelerin antioksidan aktiviteleri ABTS
[2,2'-azino-bis (3-etilbenzotiazolin-6-sülfonik asit)] ve DPPH
(2,2-difenil-1-pikrilhidrazil) yöntemleriyle spektrofotometrik olarak
belirlendi ve troloks eşdeğeri olarak hesaplandı. Antimikrobiyal aktivite disk
difüzyon yöntemiyle patojen mikroorganizmalar üzerine test edildi. Sonuç olarak
bitkinin etanol ve metanol ekstrelerinin antioksidan aktivitesi diğer
ekstrelerden anlamlı olarak yüksek bulundu (p<0.05). Benzer olarak, etanol
ve metanol ekstrelerinin antimikrobiyal aktivitesi de diğer eksterelerden
yüksek bulundu. 

Keywords

Alchemilla ellenbergiana, antimikrobiyal aktivite, ABTS, antioksidan aktivite, disk difüzyon, DPPH

Determination of Antioxidant and Antimicrobial Properties of Lady's Mantle (Alchemilla ellenbergiana) Extracts

Abstract

In this study, different polarity solvent (hexane, ethyl acetate,
ethanol and methanol) extracts of Alchemilla ellenbergiana (Lady's Mantle),
which used in folk medicine plant in Gümüşhane region, were investigated in
terms of total phenolic contents, antioxidant and antimicrobial activity.  Total phenolic contents were measured by
spectrophotometric metod as gallic acid equivalent and detect in the highest in
ethanol and methanol extracts. Antioxidant activity of extracts were determined
by ABTS [2,2′-Azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium
salt] and DPPH (2,2-diphenyl-1-picrylhydrazyl) methods by spectrophotometric
metod and calculated as trolox equivalent. Antimicrobial activity was tested
against to pathogen microorganisms by disc diffusion method. As a result, it
has been found that ethanol and methanol extracts of plant have high
antioxidant and antimicrobial activity (p<0.05). Similarly, it has been
found that antimicrobial activity of ethanol and methanol extracts higher than
others.

Keywords

ABTS, Alchemilla ellenbergiana, antimicrobial activity, antioxidant activity, disc diffusion, DPPH

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