Sıçanlarda İndometazin İle İndüklenen Mide Ülseri Üzerine Krom Pikolinatın Koruyucu Etkileri

Year 2019, Volume: 8 Issue: 3, 237 - 244, 30.09.2019

Gözde Atila Uslu Hamit Uslu

Abstract

Bu çalışmada, nonsteroid anti-inflamatuar
ilaçlardan biri olan indometazin ile indüklenen gastrik lezyonlar üzerine krom
pikolinatın (Crpic) koruyucu etkilerinin araştırılması amaçlandı. Araştırmada
40 adet dişi Sprague-Dawley sıçan kullanıldı. Sıçanlar eşit olarak dört gruba
ayrıldı, Kontrol (K), Krom pikolinat + İndomethazin (Crpic+İND), İndomethazin
(İND) and Pantoprazol + İndomethazin (PAN+İND). Sırasıyla I gruba sadece
Di-metil sülfoksit (DMSO) verildi, grup II'ye 300 µg/kg Crpic verildi, grup
III'e DMSO verildi ve IV grubuna 5 mg/kg pantoprazol verildi. Çalışmanın 11.
gününde, II, III ve IV gruplarına tek doz 100 mg/kg indometazin oral olarak
verildi. C-reaktif protein (CRP) düzeyleri, İND grubunda kontrol grubuna göre
daha yüksekti (I-III p<0,001, I-II, IV p<0,05). Ancak Crpic ve pantoprazol
gruplarında tek başına indometazin ile tedavi edilen gruba göre anlamlı
düşüşler gözlendi (p<0,01). Siklooksijenaz-2 (COX-2) düzeyleri indometazin
uygulaması ile artarken (p<0,001) Crpic (p<0,001) ve pantoprazol (p<0,01)
uygulamalarıyla anlamlı olarak azaldı. Tümör nekrozis faktör-alfa (TNF-α)
(p<0,05) ve interlökin-6 (IL-6) (p<0,01) seviyeleri, İND grubunda önemli
ölçüde artmıştır. Krom pikolinat ve pantoprazol gruplarında TNF-α ve IL-6
düzeylerinde bazı düşüşler vardı, ancak bu düşüşler anlamlı değildi. Sonuç
olarak; Krom pikolinatın, inflamasyonun patogenezinde önemli bir rol oynayan
COX-2 ve CRP seviyelerini önemli ölçüde azalttığı bulunmuştur. Ayrıca krom
pikolinatın indometazin ile indüklenen gastrik lezyonları önlemede oldukça
etkili olduğu belirlendi. 

Keywords

Mide Ülseri, Krom pikolinat, İndomethazin, Siklooksijenaz-2

Protective Effects of Chromium Picolinate on Indomethacin-Induced Stomach Ulcer in the Rat

Abstract

In the present study, it was aimed to
investigate the protective effects of chromium picolinate (Crpic) on gastric
lesions induced by indomethacine, one of the non-steroid anti-inflammatory
drugs. Forty female Sprague-Dawley rats were used in this research. The rats
were equally divided into four groups, namely Control (C), Chromium Picolinate
+ Indomethacine (Crpic+IND), Indomethacine (IND) and Pantoprazole +
Indomethacine (PAN+IND). Respectively the group I was given Dimethyl Sulfoxide
(DMSO) only, the group II was given 300 μg/kg Crpic, group III was given (DMSO)
and the group IV was given 5 mg/kg pantoprazole orally. On the 11th day of the
study, a single dose of 100 mg/kg indomethacine was administered orally to the
II, III and IV groups. C-reactive protein (CRP) levels was higher than the
control group IND group (I-III p<0.001, I-II, IV p<0.05). However,
significant decreases were observed in the Crpic and pantoprazole groups
compared to the group treated with indomethacine alone (p<0.01).
Cyclooxygenase-2 (COX-2) levels were increased by indomethacine administration
(p<0.001), whereas Crpic (p<0.001) and pantoprazole (p<0.01)
applications significantly decreased. Tumor necrosis factor-alpha (TNF-α)
(p<0.05) and interleukin-6 (IL-6) (p<0.01), levels increased significantly
in İND group. There were some decreases in TNF-α and IL-6 levels in chromium
picolinate and pantoprazole groups, but these decreases were not significant.
As a result; it has been found that chromium picolinate significantly reduces
COX-2 and CRP levels, which play an important role in the pathogenesis of
inflammation. Furthermore, it was determined that chromium picolinate was very
effective in preventing indomethacin-induced gastric lesions. 

Keywords

Stomach ulcer, Chromium picolinate, Indomethacine, Cyclooxygenase-2

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