Borik Asitin 8305C Anaplastik Tiroit Kanseri Hücrelerinde Antioksidan ve Anti-kanser Aktivitesi

Yıl 2021, Cilt: 10 Sayı: 2, 213 - 221, 28.06.2021

Melike Ersöz

https://doi.org/10.37989/gumussagbil.834614

Cited By: 2

Öz

Bu çalışmanın amacı, borik asitin 8305C insan anaplastik tiroit kanseri (ATK) hücrelerinde sitotoksik, anti-proliferatif, apoptotik ve antioksidan etkilerini değerlendirmektir. Borik asitin sitotoksisitesi 0-1000 μg/mL doz aralığında (24, 48 ve 72 saat) 8305C insan ATK hücrelerinde bir tetrazolyum testiyle (MTT) belirlendi. Hücrelerdeki proliferasyon ve apoptoz incelendi. Biyokimyasal parametreler spektrofotometrik olarak tespit edildi. 24, 48 ve 72 saat borik asit ile muamele edilen 8305C insan ATK hücrelerinin yarı-maksimum inhibisyon konsantrasyon (IC50) değerleri sırasıyla 238 µg/mL, 116 µg/mL ve 70 µg/mL olarak hesaplandı (p<0,05). Prolifere olan hücre çekirdek antijeni (PCNA) pozitif hücrelerin yüzdesi 200, 250, 300 µg/mL konsantrasyonda borik asit ile 48 saat muamele edilen hücrelerde anlamlı azalma gösterdi (p<0,01). Borik asitin 250 ve 300 µg/mL konsantrasyonlarında 24 ve 48 saatlik muamelesi, kontrol hücrelerine kıyasla 8305C ATK hücrelerinde apoptotik hücre sayısında anlamlı artış gösterdi (p<0,01). En düşük malondialdehit seviyesi 48 saat 300 µg/mL konsantrasyonda uygulanan hücrelerde saptandı (p<0,01). En yüksek süperoksit dismutaz aktivitesi 48 saat 250 µg/mL borik asit uygulanan hücrelerde olurken (p<0,01), glutatyon seviyesi 300 µg/mL borik asit uygulanan hücrelerde kontrol hücrelerine göre anlamlı olarak arttı (p<0,05). Bu çalışma ile elde edilen sonuçlar, borik asitin 8305C insan ATK hücrelerinde anti-proliferatif ve apoptotik aktiviteye sahip umut verici yeni bir terapötik ajan olabilirliğini gösterir. Çalışma in vivo deneylerle desteklenmelidir.

Anahtar Kelimeler

Apoptoz, Borik Asit, Kanser, Proliferasyon, Troit.

Teşekkür

Çalışma sürecinde katkılarından dolayı Doç. Dr. Banu Mansur’a, Gülşah Akbaş’a ve Fatma Şayan Poyraz’a teşekkür ederim.

Antioxidant and Anti-cancer Activity of Boric Acid in 8305C Anaplastic Thyroid Cancer Cells

Öz

anti-proliferative, apoptotic and antioxidant effects of boric acid in 8305C human anaplastic thyroid cancer (ATC) cells. The cytotoxicity of boric acid was determined at a dose range of 0-1000 μg/mL in the human ATC cells (24, 48, and 72 hours) by a tetrazolium test (MTT). Proliferation and apoptosis in the cells were examined. Biochemical parameters were determined spectrophotometrically. The half-maximal inhibitory concentration (IC50) values of cells treated with boric acid for 24, 48, and 72 hours were calculated as 238 µg/mL, 116 µg/mL, and 70 µg/mL, respectively (p<0.05). The percentage of proliferating cell nuclear antigen (PCNA) positive cells showed a significant decrease in the cells treated with boric acid at 200, 250, and 300 µg/mL concentrations for 48 hours (p<0.01). Boric acid administered at 250 and 300 µg/mL showed a significant increase in the percentage of apoptotic cells of 8305C ATC cells compared to control cells (p<0.01). The lowest malondialdehyde level was detected in cells treated with boric acid at 300 µg/mL for 48 hours (p<0.01). While the highest superoxide dismutase level was in cells treated with 250 µg/mL boric acid for 48 hours (p<0.01), glutathione level increased significantly in cells treated with 300 µg/mL boric acid compared to control cells (p<0.05). The results obtained from this study demonstrate that boric acid can be a promising new therapeutic agent in terms of anti-proliferative and apoptotic activities in 8305C human ATC cells. The study should be supported by in vivo experiments.

Anahtar Kelimeler

Apoptosis, Boric Acid, Cancer, Proliferation, Thyroid.

Kaynakça

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