Antioxidant properties and phenolic compounds of methanol extracts of raisin and grape seed of Horoz Karası (Vitis vinifera L)

Year 2024, , 293 - 303, 21.06.2024

Gülcan Koyuncu , Filiz Uçan Türkmen

https://doi.org/10.29050/harranziraat.1358296

Abstract

It is of great importance to determine the bioactive properties of grapes and grape products that have significant positive effects on nutrition and health. In this study, phytochemical properties, antioxidant properties by different methods and phenolic compounds of raisins and seeds methanol extracts of Horoz Karası (Vitis vinifera L) variety were determined. While the total phenolic content of the methanol extracts of raisins was 0.662 mg GAE g-1, this value was determined as 1.542 mg GAE g-1 in the seeds. The amounts of total flavonoid substance (0.15 mg RE g-1) and ascorbic acid (174.29 mg L-1) were found to be higher in the seeds. While DPPH radical scavenging activity was higher in raisins, FRAP antioxidant capacity was higher in seeds. In all antioxidant analyses, the differences between raisins and seeds were found to be statistically significant (p<0.05). The phenolic compounds found in the highest amount in raisins were catechin hydrate (406.91 mg kg-1) from flavonoids and chrysin (331.60 mg kg-1) from flavones, while caffeic acid (990.42 mg kg-1) from hydroxycinnamic acids in seeds and naringine (310.56 mg kg-1) from flavones were found. It has also been determined that the seeds contain a very high amount of phenolic compounds compared to raisins. The data obtained revealed that methanol extracts of Horoz Karası raisins and seeds can be used as a phenolic component and natural antioxidant source thanks to the bioactive properties they contain. The differences between raisins and seeds were statistically significant in all antioxidant analyses.

Keywords

Horoz Karası, Grape seed, Extraction, Antioxidant capacity, Phenolic compound.

Project Number

21-13112

Horoz Karası (Vitis vinifera L) kuru üzüm ve üzüm çekirdeği metanol ekstraktlarının antioksidan özellikleri ile fenolik bileşikleri

Abstract

Beslenme ve sağlık üzerine önemli olumlu etkileri olan üzüm ve üzüm ürünlerinin biyoaktif özelliklerinin belirlenmesi büyük önem taşımaktadır. Bu çalışmada, Horoz Karası (Vitis vinifera L) çeşidi kuru üzüm ve çekirdek metanol ekstraktlarının fitokimyasal özellikleri, farklı yöntemlerle antioksidan özellikleri ve fenolik bileşikleri belirlenmiştir. Kuru üzümlerin metanol ekstraktlarının toplam fenolik madde miktarı 0.662 mg GAE g-1 olarak bulunurken, çekirdekte bu değer 1.542 mg GAE g-1 olarak belirlenmiştir. Toplam flavonoid madde (0.15 mg RE g-1) ve askorbik asit (174.29 mg L-1) miktarları çekirdekte daha yüksek tespit edilmiştir. DPPH radikal giderme aktivitesi kuru üzümde daha fazla bulunurken, FRAP antioksidan kapasitesi çekirdekte daha yüksek bulunmuştur. Tüm antioksidan analizlerinde kuru üzüm ve çekirdek arasındaki farklar istatistiksel olarak önemli bulunmuştur (p<0.05). Kuru üzümlerde en yüksek miktarda bulunan fenolik bileşikler flavonoidlerden kateşin hidrat (406.91 mg kg-1) ve flavonlardan krisin (331.60 mg kg-1) olurken, çekirdeklerde hidroksisinamik asitlerden kafeik asit (990.42 mg kg-1) ve flavonlardan narinjin (310.56 mg kg-1) olmuştur. Çekirdeklerin kuru üzüme kıyasla oldukça yüksek miktarda fenolik bileşik içerdiği de tespit edilmiştir. Elde edilen veriler Horoz Karası kuru üzüm ve çekirdeklerinin metanol ekstraktlarının içerdikleri biyoaktif özellikler sayesinde fenolik bileşen ve doğal antioksidan kaynağı olarak kullanılabileceğini ortaya koymuştur.

Keywords

Horoz Karası, Üzüm çekirdeği, Ekstraksiyon, Antioksidan kapasitesi, Fenolik bileşik

Supporting Institution

Kilis 7 Aralık Üniversitesi BAP Birimi

Project Number

21-13112

Thanks

Kilis 7 Aralık Üniversitesi BAP birimine (Proje No: 21-13112) ve fenolik bileşen tayininde yarıdımcı olan Öğr. Gör. Dr. Ümit Haydar EROL’a teşekkür ederiz.

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