Optimization of Microwave-assisted Extraction of Phenolics from Organic Strawberry Using Response Surface Methodology

Year 2017, Volume 21, Issue 2, 143 - 154, 19.06.2017

Aysel ELİK Derya KOÇAK YANIK Fahrettin GÖĞÜŞ

https://doi.org/10.29050/harranziraat.321124

Abstract

The effects of microwave- assisted extraction (MAE) were investigated on extraction of phenolic compounds from strawberry fruit. Response surface methodology (RSM) was used to optimize the extraction conditions. A face-centered central composite design (FCCCD) was employed to determine the effects of independent variables such as microwave power (100-300 W), extraction time (2-16 min) and solvent to sample ratio (5:1-25:1 mL g^-1) on the extraction yield and total phenolic content (TPC). Optimized conditions were determined as 265 W of microwave power, 2 min of extraction time and 24:1 mL g^-1 of solvent to sample ratio. The maximum predicted extraction yield and TPC under the optimum conditions were 8.23 % and 19.65 mg GAE g^-1 dry strawberry, respectively. Total anthocyanin content (TAC), DPPH·EC₅₀ and FRAP values of extracts produced at optimum conditions were determined as 2.3 mg Cyn-3-glu g^-1 dry strawberry, 1.67 mg dry strawberry mL^-1 and 197.83 μmoles TE g^-1 dry strawberry, respectively.

Keywords

Microwave-assisted extraction, Strawberry, Phenolics, Response surface methodology

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