Polymorphism of the Calpastatin (CAST) and Growth Differentiation Factor 9 (GDF9) genes in Akkaraman Sheep Breed

Year 2022, , 21 - 26, 01.07.2022

Koray Kırıkçı

https://doi.org/10.29185/hayuretim.1008768

Cited By: 1

Abstract

Objective: In this study, the gene polymorphisms of Calpastatin (CAST) and Growth Differentiation factor 9 (GDF9) were determined in Akkaraman sheep breed.
Material and Methods: Genomic DNA was obtained from blood samples of 50 Akkaraman sheep. All samples for CAST and GDF9 were genotyped by the Polymerase Chain Reaction-Restriction Fragment Length Polymorphism (PCR-RFLP) method using the enzymes MspI and Hhal.
Results: Three (MM, MN, and NN) and two (GG and GA) genotypes, respectively, were detected at the CAST and GDF9 genes. The frequencies of the genotypes MM, MN and NN were determined to be 44 %, 54 % and 2 %, respectively. The frequencies of the GG and GA genotypes were found to be 84 % and 16 %, respectively. The AA genotype was not found.
Conclusion: The study showed for the first time that the Akkaraman breed carries the GDF9 G1 mutation and has sufficient variation in the CAST. Previous studies provided that both genes can be used as biomarkers for increasing lambing yield and growth rates. Therefore, in order for the studied genes to be used as biomarkers in the Akkaraman breed, association studies should first be performed. 

Keywords

Akkaraman koyunu, CAST/Msp1, GDF9/Hha1, PCR-RFLP

Supporting Institution

Bulunmamaktadır

Project Number

Bulunmamaktadır

Thanks

The author would like to thank the General Directorate of Agricultural Research and Policy of the Ministry of Agriculture and Forestry and the Yozgat Provincial Sheep Breeders Association and the technical staff of the project for their support. The author also thanks Ms. Melike Kavuzkoz, a trainee of molecular biology and genetics at Kırşehir Ahi Evran for her help in laboratory analysis.

Akkaraman Koyun Irkında Kalpastatin (CAST) ve Büyüme Farklılaşma Faktörü 9 (GDF9) Genlerin Polimorfizmi

Abstract

Objective: Bu çalışmada, Kalpastatin ve Büyüme Farklılaşma Faktörü 9 gen polimorfizmleri Akkaraman koyun ırkında belirlenmiştir.
Materyal ve Metot: Genomik DNA 50 baş Akkaraman koyun ırkına ait kan numunelerinden elde edilmiştir. Kalpastatin ve Büyüme Farklılaşma Faktörü 9 genleri için tüm örnekler, sırasıyla MspI ve Hhal enzimleri kullanılarak Polimeraz Zincir Reaksiyon- Restriksiyon Parça Uzunluk Polimorfizm yöntemi ile genotiplendirilmiştir.
Bulgular: Kalpastatin ve Büyüme Farklılaşma Faktörü 9 geninde sırasıyla üç (MM, MN ve NN) ve iki genotip (GG ve GA) grupu tanımlanmıştır. Kalpastatin locusunda, MM, MN ve NN genotiplerin frekansları sırasıyla % 44, % 54 ve % 2 olarak belirlenmiştir. Büyüme Farklılaşma Faktörü 9 lokusunda ise GG ve GA genotiplerin frekansı ise % 84 ve % 16 olarak bulunmuştur. AA genotipine ise rastlanılmamıştır
Sonuç: Çalışma ilk defa Akkaraman koyun ırkının GDF9-G1 mutasyonunu taşıdığını ve CAST geni bakımından yeterli derecede genetik varyasona sahip olduğunu göstermiştir. Önceki araştırmalar her iki genin kuzu verimi ve büyüme oranlarının arttırılabilmesi amacıyla biyomarkör olarak kullanılabileceğini göstermiştir. Sonuç olarak, incelenen genlerin Akkaraman ırkında biyomarkör olarak kullanılabilmesi için öncelikle ilişkilendirme çalışmalarının yapılması önerilmektedir.

Keywords

Akkaraman koyunu, CAST/Msp1, GDF9/Hha1, PCR-RFLP

Project Number

Bulunmamaktadır

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