Polymorphism of the Calpastatin (CAST) and Growth Differentiation Factor 9 (GDF9) genes in Akkaraman Sheep Breed

Yıl 2022, , 21 - 26, 01.07.2022

Koray Kırıkçı

https://doi.org/10.29185/hayuretim.1008768

Cited By: 1

Öz

Objective: In this study, the gene polymorphisms of Calpastatin (CAST) and Growth Differentiation factor 9 (GDF9) were determined in Akkaraman sheep breed.
Material and Methods: Genomic DNA was obtained from blood samples of 50 Akkaraman sheep. All samples for CAST and GDF9 were genotyped by the Polymerase Chain Reaction-Restriction Fragment Length Polymorphism (PCR-RFLP) method using the enzymes MspI and Hhal.
Results: Three (MM, MN, and NN) and two (GG and GA) genotypes, respectively, were detected at the CAST and GDF9 genes. The frequencies of the genotypes MM, MN and NN were determined to be 44 %, 54 % and 2 %, respectively. The frequencies of the GG and GA genotypes were found to be 84 % and 16 %, respectively. The AA genotype was not found.
Conclusion: The study showed for the first time that the Akkaraman breed carries the GDF9 G1 mutation and has sufficient variation in the CAST. Previous studies provided that both genes can be used as biomarkers for increasing lambing yield and growth rates. Therefore, in order for the studied genes to be used as biomarkers in the Akkaraman breed, association studies should first be performed. 

Anahtar Kelimeler

Akkaraman koyunu, CAST/Msp1, GDF9/Hha1, PCR-RFLP

Destekleyen Kurum

Bulunmamaktadır

Proje Numarası

Bulunmamaktadır

Teşekkür

The author would like to thank the General Directorate of Agricultural Research and Policy of the Ministry of Agriculture and Forestry and the Yozgat Provincial Sheep Breeders Association and the technical staff of the project for their support. The author also thanks Ms. Melike Kavuzkoz, a trainee of molecular biology and genetics at Kırşehir Ahi Evran for her help in laboratory analysis.

Akkaraman Koyun Irkında Kalpastatin (CAST) ve Büyüme Farklılaşma Faktörü 9 (GDF9) Genlerin Polimorfizmi

Öz

Objective: Bu çalışmada, Kalpastatin ve Büyüme Farklılaşma Faktörü 9 gen polimorfizmleri Akkaraman koyun ırkında belirlenmiştir.
Materyal ve Metot: Genomik DNA 50 baş Akkaraman koyun ırkına ait kan numunelerinden elde edilmiştir. Kalpastatin ve Büyüme Farklılaşma Faktörü 9 genleri için tüm örnekler, sırasıyla MspI ve Hhal enzimleri kullanılarak Polimeraz Zincir Reaksiyon- Restriksiyon Parça Uzunluk Polimorfizm yöntemi ile genotiplendirilmiştir.
Bulgular: Kalpastatin ve Büyüme Farklılaşma Faktörü 9 geninde sırasıyla üç (MM, MN ve NN) ve iki genotip (GG ve GA) grupu tanımlanmıştır. Kalpastatin locusunda, MM, MN ve NN genotiplerin frekansları sırasıyla % 44, % 54 ve % 2 olarak belirlenmiştir. Büyüme Farklılaşma Faktörü 9 lokusunda ise GG ve GA genotiplerin frekansı ise % 84 ve % 16 olarak bulunmuştur. AA genotipine ise rastlanılmamıştır
Sonuç: Çalışma ilk defa Akkaraman koyun ırkının GDF9-G1 mutasyonunu taşıdığını ve CAST geni bakımından yeterli derecede genetik varyasona sahip olduğunu göstermiştir. Önceki araştırmalar her iki genin kuzu verimi ve büyüme oranlarının arttırılabilmesi amacıyla biyomarkör olarak kullanılabileceğini göstermiştir. Sonuç olarak, incelenen genlerin Akkaraman ırkında biyomarkör olarak kullanılabilmesi için öncelikle ilişkilendirme çalışmalarının yapılması önerilmektedir.

Anahtar Kelimeler

Akkaraman koyunu, CAST/Msp1, GDF9/Hha1, PCR-RFLP

Proje Numarası

Bulunmamaktadır

Kaynakça

• Aboelhassan DM, Darwish AM, Ali NI, Ghaly IS, Farag IM. 2021. A study on mutation points of GDF9 gene and their association with prolificacy in Egyptian small ruminants. Journal of Genetic Engineering and Biotechnology, 19(1): 1-11.
• Aktaş AH, Halıcı İ, Doğan Ş, Demirci U, Ali ATİK, Yaylacı E, Recep ÇİL. 2016. Akkaraman Koyunların Yetiştirici Şartlarındaki Döl Verimleri, Canlı Ağırlıkları ve Bazı Vücut Ölçüleri. Hayvansal Üretim, 57(1): 7-14.
• Avanus K. 2015. Genetic variability of CAST gene in native sheep breeds of Turkey. Kafkas Üniversitesi Veteriner Fakültesi Dergisi, 21(6): 789-794.
• Balcıoğlu MS, Karslı T, Șahin E, Ulutaș, Z, Aksoy Y. 2014. Determination of calpastatin (CAST) gene polimorphism in some native sheep breeds reared in Turkey by PCR-RFLP method. Tarim Bilimleri Dergisi, 20(4): 427-433.
• Bayram D, Akyüz B, Arslan K, Özdemir F, Aksel EG, Çınar, MU. 2019. DGAT1, CAST and IGF-I gene polymorphisms in Akkaraman lambs and their effects on live weights up to weaning age. Kafkas Üniversitesi Veteriner Fakültesi Dergisi, 25(1): 9-15.
• Bradford GE. 1985. In Genetics of Reproduction in Sheep, eds. Land R.B. and Robinson D.W. 3-18.
• Calus MPL. 2013. Predicted accuracy of and response to genomic selection for new traits in dairy cattle. Animal: an international journal of animal bioscience 7(2):183-191.
• Hill W.G. 2000. Maintenance of quantitative genetic variation in animal breeding programmes. Livestock Production Science 63(2): 99-109.
• Donicer Montes V, Claudia Lenis V, Darwin Hernández H. 2019. Polymorphisms of the calpain and calpastatin genes in two populations of colombian creole sheep. Revista MVZ Córdoba, 24(1): 7113-7118.
• Donicer Montes V, Claudia Lenis V, Darwin Hernández H. 2019. Polymorphisms of the calpain and calpastatin genes in two populations of colombian creole sheep. Revista MVZ Córdoba, 24(1): 7113-7118.
• Eghbalsaied S, Khorasgani FR, Amini HR, Farahi M, Davari M, Pirali A, Pourali S, Vatankhah M, Rostami M, Atashi H. 2017. Variant GDF9 mRNA is likely not the main cause of larger litter size in Iranian Lori-Bakhtyari, Shal, Ghezel, and Afshari sheep breeds. Archives Animal Breeding, 60(2): 119-129.
• Gbangboche AB, Adamou-Ndiaye M, Youssao AKI, Farnir F, Detilleux J, Abiola FA, Leroy PL. 2006. Non-genetic factors affecting the reproduction performance, lamb growth and productivity indices of Djallonke sheep. Small Ruminant Research, 64(1-2): 133-142.
• Getmantseva L, Bakoev, N, Shirokova N, Kolosova M, Bakoev S, Kolosov A, Usatov A, Shevtsova V, Kolosov Y. 2019. Effect of the GDF9 gene on the weight of lambs at birth. Bulgarian Journal of Agricultural Science, 25(1): 153-157.
• Gorlov IF, Kolosov YA, Shirokova NV, Getmanseva LV, Slozhenkina MI, Mosolova NI, Bakoev NF, Leonova MA, Kolosov AY, Zlobina EY. 2018. GDF9 gene polymorphism and its association with litter size in two Russian sheep breeds. Rendiconti Lincei. Scienze Fisiche e Naturali, (29): 61–66.
• Gursel E. 2011. Determination of BMP-15, BMPR-1B and GDF-9 gene mutations of the indigenous sheep breeds in Turkey. Kafkas Üniversitesi Veteriner Fakültesi Dergisi, 17(5): 725-729.
• Hanrahan JP, Gregan SM, Mulsant P, Mullen M, Davis GH, Powell R, Galloway SM. 2004. Mutations in the genes for oocyte-derived growth factors GDF9 and BMP15 are associated with both increased ovulation rate and sterility in Cambridge and Belclare sheep (Ovis aries). Biology of reproduction, 70(4): 900-909.
• Karsli T, Sahin ., Karsli BA, Alkan S, Balcioglu MS. 2012. An investigation of mutations (FecXG, FecXI, FecXH, FecXB) on BMP-15 gene in some local sheep breeds raised in Turkey. Akdeniz Üniv. Ziraat Fak, 25(1): 29-33.
• Karslı T, Şahin E, Karsli BA, Eren MG, Balcioğlu MS. 2011. Kangal ve Güney Karaman koyunlarında FecB, FecXG, FecXH Allellerinin PZR-RFLP yöntemi kullanılarak araştırılması. Lalahan Hayvancılık Araştırma Enstitüsü Dergisi, 51(2): 71-80. Kasiriyan MM, Hafazian SH, Hassani N. 2011. Genetic polymorphism BMP15 and GDF9 genes in Sangsari sheep of Iran. International Journal of Genetics and Molecular Biology, 3(1): 31-34.
• Kırıkçı K, Çam MA, Mercan L. 2021a. Investigation of the CAST Gene Polymorphism in Karayaka Sheep. Manas Journal of Agriculture Veterinary and Life Sciences, 11(1): 89-93.
• Kirikci K, Cam MA, Mercan L. 2021b. Investigation of G1 (c. 260G> A) polymorphism in exon 1 of GDF9 gene in Turkish sheep breed Karayaka. Turkish Journal of Veterinary and Animal Sciences, 45(1): 191-197.
• Kulikova K, Yuldashbaev Y, Hatataev S. 2018. The polymorphism of Cast and GDF9 genes in the Tuvan short-fat-tailed sheep population. Scientific Papers-Series D-Animal Science, 61(1): 14-17.
• OECD. 2021. OECD-FAO Agricultural Outlook, https://data.oecd.org/agroutput/meat-consumption.htm (October 2021).
• Palmer BR, Roberts N, Hickford JG, Bickerstaffe R. 1998. Rapid communication: PCR-RFLP for MspI and NcoI in the ovine calpastatin gene. Journal of Animal Science, 76(5): 1499-1500.
• Polley S, De S, Brahma B, Mukherjee A, Vinesh PV, Batabyal S, Arora JS, Pan S, Samanta AK, Datta TK, Goswami SL. 2010. Polymorphism of BMPR1B, BMP15 and GDF9 fecundity genes in prolific Garole sheep. Tropical animal health and production, 42(5): 985-993.
• Ramadevi B, Kumari B, Sudhakar K, Gangaraju G, Vinod U. 2020. Polymorphism of the Ovine Calpastatin (CAST) gene and its association with productive traits in Nellore sheep. Journal of Animal Research, 10(6): 881-887.
• Sutikno S, Yamin M, Sumantri, C. 2011. Association of polymorphisms calpastatin gene with body weight of local sheep in Jonggol, Indonesia. Media Peternakan, 34(1): 1-1.
• Tekerli M, Gündoğan M, Akıncı Z, Akcan A. 2002. Akkaraman, Dağlıç, Sakız ve İvesi koyunlarının Afyon koşullarındaki verim özelliklerinin belirlenmesi. Lalahan Hayvancılık Araştırma Enstitüsü Dergisi, 42(2): 29-36.
• Yeh FC, Yang RC, Boyle TBJ, Ye ZH, Mao JX. POPGENE. 1997. The User-Friendly Shareware for Population Genetic Analysis. Edmonton, Canada: University of Alberta.
• Yilmaz O, Cemal I, Karaca O, Ata N 2014. Association of Calpastatin (CAST) gene polymorphism with weaning weight and ultrasonic measurements of loin eye muscle in Kıvırcık lambs. Journal of the Faculty of Veterinary Medicine, Kafkas University, 20(5): 675-680.
• Yilmaz O, Sezenler T, Ata N, Yaman Y, Cemal I, Karaca O. 2014. Polymorphism of the ovine calpastatin gene in some Turkish sheep breeds. Turkish Journal of Vetetinary and Animal Sciences, 38 (4): 354-357.