Eklem Kıkırdağı Hasarının Tedavisinde Rejeneratif Materyal: Hyaluronik Asit ve Poli(glikolik asit) Kompozit Matrislerin Sentezi ve Karakterizasyonu

Abstract

Günümüzde eklem kıkırdağı bozukluklarıyla ilişkili ağrıyı hafifletmek için analjezikler, anti-inflamatuar ilaçlar ve hyaluronik asit viskosuplementasyonu kullanılmaktadır. Hyaluronik asit enjeksiyonları sadece ağrıyı azaltıcı etkileriyle değil aynı zamanda kıkırdak rejenerasyonunu uyarmasıyla da bilinmektedir. Bu çalışmada, mikro kırık ve subkondral kemik stimülasyonu sonrası dejenerasyona uğramış eklem kıkırdağının onarımı için poli(glikolik asit) ağ ve çapraz bağlı hyaluronik asitten oluşan rejeneratif bir biyomalzeme platformu geliştirilmiştir. Bu amaçla, ilk aşamada 23 mg/mL konsantrasyon içeren bütandiol diglisidil eter ile çapraz bağlı hyaluronik asit jelleri hazırlanmıştır. Elde edilen jellerdeki kalıntı bütandiol diglisidil eter çapraz bağlayıcısı 1 ppb'nin altında elde edilmiştir. pH değeri 6,95 ± 0,2 ve ozmolalite 361,3 ± 2,9 mOsm/kg olarak belirlenmiştir. Enjeksiyon kuvveti ve ilgili reolojik özellikler incelenmiştir. İkinci aşamada, çapraz bağlı hyaluronik asit jelleri poli(glikolik asit) ağlara emdirilmiş, taramalı elektron mikroskobu kullanılarak incelenmiş ve kimyasal olarak karakterize edilmiştir. Son olarak, kompozit matrisler kondrositlerle reselülerize edilmiş ve hücre canlılığı analizi Alamar Blue kullanılarak gerçekleştirilmiştir. Poli(glikolik asit) ağ ve çapraz bağlı hyaluronik asitten oluşan kompozit yapının Alamar Blue sonuçları ve taramalı elektron mikroskobu görüntüleri kompozit yapının kondrosit canlılığını desteklediğini göstermiştir.

Keywords

• Biyomateryal
• Kıkırdak rekonstrüksiyonu
• Hyaluronik Asit
• Poli(glikolik asit)

Regenerative Material for the Treatment of Articular Cartilage Damage: Synthesis and Characterization of Hyaluronic Acid and Poly(glycolic acid) Composite Matrices

Abstract

Currently, analgesics, anti-inflammatory drugs, and hyaluronic acid viscosupplementation are used to alleviate pain associated with joint cartilage disorders. Hyaluronic acid injections are known not only for their pain-reducing effects but also for stimulating cartilage regeneration. In this study, a regenerative biomaterial platform comprising poly (glycolic acid) mesh and cross-linked hyaluronic acid was developed for the repair of degenerated joint cartilage following microfracture and subchondral bone stimulation. For this purpose, in the first stage, hyaluronic acid gels cross-linked with butanediol diglycidyl ether, containing a concentration of 23 mg/mL, were prepared. The residual butanediol diglycidyl ether cross-linker in the obtained gels was below 1 ppb. The pH value was determined to be 6.95 ± 0.2, and the osmolality was 361.3 ± 2.9 mOsm/kg. The injection force and related rheological properties were investigated. In the second stage, the cross-linked hyaluronic acid gels were impregnated into poly (glycolic acid) meshes, evaluated using scanning electron microscopy and characterized chemically. Finally, the composite matrices were recellularized with chondrocytes, and cell viability analysis was conducted using Alamar Blue. The Alamar Blue results and scanning electron microscopy images of the composite structure consisting of poly (glycolic acid) mesh and cross-linked hyaluronic acid indicated that the structure supports chondrocyte viability.

Keywords

• Biomaterial
• Cartilage Reconstruction
• Hyaluronic Acid
• Poly(glycolic acid)

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