N. Nishida, H. Yano, T. Nishida, T. Kamura, M. Kojiro, Angiogenesis in cancer, Vasc. Health Risk Manag. 2 (2006) 213–219.
S.Y. Yoo, S.M. Kwon, Angiogenesis and its therapeutic opportunities, Mediators Inflamm. 127170 (2013) 1-11.
N. Rahimi, K. Rezazadeh, J.E. Mahoney, E. Hartsough, R.D. Meyer, Identification of IGPR-1 as a novel adhesion molecule involved in angiogenesis, Mol. Biol. Cell. 23 (2012) 1646-1656.
Y. Cao, Y. Gong, L. Liu, Y. Zhou, X. Fang, C. Zhang, Y. Li, J. Li, The use of human umbilical vein endothelial cells (HUVECs) as an in vitro model to assess the toxicity of nanoparticles to endothelium: a review, J. Appl. Toxicol. 37 (2017) 1359-1369.
C. He, X. Sun, H. Qiao, X. Jiang, D. Wang, X. Jin, X. Dong, J. Wang, H. Jiang, X. Sun, Downregulating hypoxia‐inducible factor‐2α improves the efficacy of doxorubicin in the treatment of hepatocellular carcinoma, Cancer Sci. 103 (2012) 528-534.
C. Belger, C. Abrahams, A. Imamdin, S. Lecour, Doxorubicin-induced cardiotoxicity and risk factors, Int. J. Cardiol. Heart Vasc. 50 (2023) 101332.
R. Mattioli, A. Ilari, B. Colotti, L. Mosca, F. Fazi, G. Colotti, Doxorubicin and other anthracyclines in cancers: Activity, chemoresistance and its overcoming, Mol. Aspects Med. 93 (2023) 101205.
K. Elumalai, S. Srinivasan, A. Shanmugam, Review of the efficacy of nanoparticle-based drug delivery systems for cancer treatment, Biomed. Technol. 5 (2024) 109-122.
T.D. Malouff, D.S. Seneviratne, D.K. Ebner, W.C. Stross, M.R. Waddle, D.M. Trifiletti, S. Krishnan, Boron neutron capture therapy: A review of clinical applications. Front Oncol. 26(11) (2021) 601820.
D. Çetin Altındal, Therapeutic potential of boron-based nanoparticles for enhanced glioblastoma treatment, J Drug Deliv. Sci. Tec. 99 (2024) 105936.
K. Nedunchezhian, N. Aswath, M. Thiruppathy, S. Thirugnanamurthy, Boron neutron capture therapy - A literature review, J. Clin. Diagn. Res. 10 (2016) ZE01-ZE04.
J. Folkman, Role of angiogenesis in tumor growth and metastasis, Semin. Oncol. 29 (2022) 15-18.
A.R. Gada, P. Vaswani, R. Singh, D. Bhatia, Self-assembled DNA nanocages promote cell migration and differentiation of human umbilical vein endothelial cells, Chembiochem 24 (2023) e202200634.
M.A. Abou El Hassan, H.M. Verheul, A.S. Jorna, C. Schalkwijk, J. van Bezu, W.J. van der Vijgh, A. Bast, The new cardioprotector monohydroxyethylrutoside protects against doxorubicin-induced inflammatory effects in vitro, Br. J. Cancer 89 (2003) 357-362.
M. Peng, M. Yang, Y. Ding, L. Yu, Y. Deng, W. Lai, Y. Hu, Mechanism of endogenous digitalis-like factor induced vascular endothelial cell damage in patients with severe preeclampsia, Int. J. Mol. Med. 41 (2018) 985-994.
C. Duy, M. Li, M. Teater, C. Meydan, F.E. Garrett-Bakelman, T.C. Lee, C.R. Chin, C. Durmaz, K.C. Kawabata, E. Dhimolea, C.S. Mitsiades, H. Doehner, R.J. D’Andrea, M.W. Becker, E.M. Paietta, C.E. Mason, M. Carroll, A.M. Melnick, Chemotherapy induces senescence-like resilient cells capable of initiating AML recurrence. Cancer Discov. 11(6) (2021) 1542-1561.
E. Lorenzo, C. Ruiz-Ruiz, A.J. Quesada, G. Hernández, A. Rodríguez, A. López-Rivas, J.M. Redondo, Doxorubicin induces apoptosis and CD95 gene expression in human primary endothelial cells through a p53-dependent mechanism, J. Biol. Chem. 277 (2002) 10883-1092.
Effect of Doxorubicin-Loaded Tetraborate Nanoparticles on HUVEC Behavior for Tumor Angiogenesis
Angiogenez, tümör büyümesini destekler ve yakın dokulara metastazı kolaylaştırır, bu nedenle inhibisyonu kanser tedavisinde ana hedeflerden biridir. İnsan göbek kordonu venöz endotel hücreleri (HUVECler), kan damarlarının oluşumundaki rolleri nedeniyle in vitro angiogenez çalışmalarında sıklıkla kullanılmaktadır. Bu çalışma, doxorubisin (Dox) ile yüklenmiş kalsiyum tetraborat nanopartikülleri (CaB4O7-Dox) ve magnezyum tetraborat nanopartiküllerinin (MgB4O7-Dox) HUVEC davranışları üzerindeki etkilerini incelemekte olup, hücre proliferasyonunu inhibe etmeyi ve kanser tedavi etkinliğini artırmayı amaçlamaktadır. CaB4O7 ve MgB4O7 nanopartiküllerinin boyutları sırasıyla yaklaşık 200 nm ve 300 nm olarak belirlenmiştir. Sitotoksisite çalışmaları, Dox'un HUVECler için yaklaşık 350 ng/mL (440 nM) IC50 değerine sahip olduğunu göstermiştir. Dox ile yüklenmiş kalsiyum ve magnezyum tetraborat nanopartikülleri, HUVEC proliferasyonunu inhibe etmede serbest Dox ve kontrol gruplarına göre belirgin derecede üstündür. Ayrıca, bu nanopartiküller hücre morfolojisini önemli ölçüde değiştirmiş ve tüp oluşum eğilimini bozmuştur, bu da anti-anjiyogenik etkilerini göstermektedir. Bu bulgular, Dox ile yüklenmiş tetraborat nanopartiküllerinin HUVEC davranışını ve anjiyogenezini etkili bir şekilde modüle ettiğini ve bu nanopartikül bazlı sistemlerin kanser tedavilerinin etkinliğini artırma potansiyelini vurgulamaktadır.
Bu çalışmanın, özgün bir çalışma olduğunu; çalışmanın hazırlık, veri toplama, analiz ve bilgilerin sunumu olmak üzere tüm aşamalarından bilimsel etik ilke ve kurallarına uygun davrandığımı beyan ederim.
Supporting Institution
TÜBİTAK
Project Number
Tübitak 120Z954
References
N. Nishida, H. Yano, T. Nishida, T. Kamura, M. Kojiro, Angiogenesis in cancer, Vasc. Health Risk Manag. 2 (2006) 213–219.
S.Y. Yoo, S.M. Kwon, Angiogenesis and its therapeutic opportunities, Mediators Inflamm. 127170 (2013) 1-11.
N. Rahimi, K. Rezazadeh, J.E. Mahoney, E. Hartsough, R.D. Meyer, Identification of IGPR-1 as a novel adhesion molecule involved in angiogenesis, Mol. Biol. Cell. 23 (2012) 1646-1656.
Y. Cao, Y. Gong, L. Liu, Y. Zhou, X. Fang, C. Zhang, Y. Li, J. Li, The use of human umbilical vein endothelial cells (HUVECs) as an in vitro model to assess the toxicity of nanoparticles to endothelium: a review, J. Appl. Toxicol. 37 (2017) 1359-1369.
C. He, X. Sun, H. Qiao, X. Jiang, D. Wang, X. Jin, X. Dong, J. Wang, H. Jiang, X. Sun, Downregulating hypoxia‐inducible factor‐2α improves the efficacy of doxorubicin in the treatment of hepatocellular carcinoma, Cancer Sci. 103 (2012) 528-534.
C. Belger, C. Abrahams, A. Imamdin, S. Lecour, Doxorubicin-induced cardiotoxicity and risk factors, Int. J. Cardiol. Heart Vasc. 50 (2023) 101332.
R. Mattioli, A. Ilari, B. Colotti, L. Mosca, F. Fazi, G. Colotti, Doxorubicin and other anthracyclines in cancers: Activity, chemoresistance and its overcoming, Mol. Aspects Med. 93 (2023) 101205.
K. Elumalai, S. Srinivasan, A. Shanmugam, Review of the efficacy of nanoparticle-based drug delivery systems for cancer treatment, Biomed. Technol. 5 (2024) 109-122.
T.D. Malouff, D.S. Seneviratne, D.K. Ebner, W.C. Stross, M.R. Waddle, D.M. Trifiletti, S. Krishnan, Boron neutron capture therapy: A review of clinical applications. Front Oncol. 26(11) (2021) 601820.
D. Çetin Altındal, Therapeutic potential of boron-based nanoparticles for enhanced glioblastoma treatment, J Drug Deliv. Sci. Tec. 99 (2024) 105936.
K. Nedunchezhian, N. Aswath, M. Thiruppathy, S. Thirugnanamurthy, Boron neutron capture therapy - A literature review, J. Clin. Diagn. Res. 10 (2016) ZE01-ZE04.
J. Folkman, Role of angiogenesis in tumor growth and metastasis, Semin. Oncol. 29 (2022) 15-18.
A.R. Gada, P. Vaswani, R. Singh, D. Bhatia, Self-assembled DNA nanocages promote cell migration and differentiation of human umbilical vein endothelial cells, Chembiochem 24 (2023) e202200634.
M.A. Abou El Hassan, H.M. Verheul, A.S. Jorna, C. Schalkwijk, J. van Bezu, W.J. van der Vijgh, A. Bast, The new cardioprotector monohydroxyethylrutoside protects against doxorubicin-induced inflammatory effects in vitro, Br. J. Cancer 89 (2003) 357-362.
M. Peng, M. Yang, Y. Ding, L. Yu, Y. Deng, W. Lai, Y. Hu, Mechanism of endogenous digitalis-like factor induced vascular endothelial cell damage in patients with severe preeclampsia, Int. J. Mol. Med. 41 (2018) 985-994.
C. Duy, M. Li, M. Teater, C. Meydan, F.E. Garrett-Bakelman, T.C. Lee, C.R. Chin, C. Durmaz, K.C. Kawabata, E. Dhimolea, C.S. Mitsiades, H. Doehner, R.J. D’Andrea, M.W. Becker, E.M. Paietta, C.E. Mason, M. Carroll, A.M. Melnick, Chemotherapy induces senescence-like resilient cells capable of initiating AML recurrence. Cancer Discov. 11(6) (2021) 1542-1561.
E. Lorenzo, C. Ruiz-Ruiz, A.J. Quesada, G. Hernández, A. Rodríguez, A. López-Rivas, J.M. Redondo, Doxorubicin induces apoptosis and CD95 gene expression in human primary endothelial cells through a p53-dependent mechanism, J. Biol. Chem. 277 (2002) 10883-1092.
There are 17 citations in total.
Details
Primary Language
English
Subjects
Cell Development, Proliferation and Death, Cellular Interactions, Biochemistry and Cell Biology (Other)
Çetin Altındal, D. (2025). Effect of Doxorubicin-Loaded Tetraborate Nanoparticles on HUVEC Behavior for Tumor Angiogenesis. Hacettepe Journal of Biology and Chemistry, 53(1), 87-96. https://doi.org/10.15671/hjbc.1532663
AMA
Çetin Altındal D. Effect of Doxorubicin-Loaded Tetraborate Nanoparticles on HUVEC Behavior for Tumor Angiogenesis. HJBC. January 2025;53(1):87-96. doi:10.15671/hjbc.1532663
Chicago
Çetin Altındal, Damla. “Effect of Doxorubicin-Loaded Tetraborate Nanoparticles on HUVEC Behavior for Tumor Angiogenesis”. Hacettepe Journal of Biology and Chemistry 53, no. 1 (January 2025): 87-96. https://doi.org/10.15671/hjbc.1532663.
EndNote
Çetin Altındal D (January 1, 2025) Effect of Doxorubicin-Loaded Tetraborate Nanoparticles on HUVEC Behavior for Tumor Angiogenesis. Hacettepe Journal of Biology and Chemistry 53 1 87–96.
IEEE
D. Çetin Altındal, “Effect of Doxorubicin-Loaded Tetraborate Nanoparticles on HUVEC Behavior for Tumor Angiogenesis”, HJBC, vol. 53, no. 1, pp. 87–96, 2025, doi: 10.15671/hjbc.1532663.
ISNAD
Çetin Altındal, Damla. “Effect of Doxorubicin-Loaded Tetraborate Nanoparticles on HUVEC Behavior for Tumor Angiogenesis”. Hacettepe Journal of Biology and Chemistry 53/1 (January 2025), 87-96. https://doi.org/10.15671/hjbc.1532663.
JAMA
Çetin Altındal D. Effect of Doxorubicin-Loaded Tetraborate Nanoparticles on HUVEC Behavior for Tumor Angiogenesis. HJBC. 2025;53:87–96.
MLA
Çetin Altındal, Damla. “Effect of Doxorubicin-Loaded Tetraborate Nanoparticles on HUVEC Behavior for Tumor Angiogenesis”. Hacettepe Journal of Biology and Chemistry, vol. 53, no. 1, 2025, pp. 87-96, doi:10.15671/hjbc.1532663.
Vancouver
Çetin Altındal D. Effect of Doxorubicin-Loaded Tetraborate Nanoparticles on HUVEC Behavior for Tumor Angiogenesis. HJBC. 2025;53(1):87-96.