Kitosan-Altın Kompozit ve İyonik Sıvı Modifiye Tek Kullanımlık Elektrotlar ile Kurkumin-DNA Etkileşiminin İncelenmesi

Abstract

Kurkumin-nükleik asit etkileşiminin elektrokimyasal yöntemle tayinine yönelik, kalem grafit elektrot (PGE) yüzeyi kitosan (CHIT), altın kompozit çözeltisi ve iyonik sıvı (IL) modifikasyonu ile yenilikçi bir sensör tasarlanmıştır. Geliştirilen CHIT-Au-IL modifiye elektrotların (CHIT-Au-IL-PGE) elektrokimyasal davranışı, döngüsel voltametri (CV) ve elektrokimyasal empedans spektroskopisi (EIS) gibi teknikler kullanılarak incelenmiştir. Ayrıca, elektrotların yüzey morfolojisini ve yapısal özelliklerini analiz etmek için taramalı elektron mikroskobu (SEM) kullanılmıştır. Kurkumin ve nükleik asitler arasındaki etkileşim üzerine optimizasyon çalışmaları elektrokimyasal teknikler kullanılarak gerçekleştirilmiştir. CHIT-Au-IL-PGE yüzeyindeki DNA konsantrasyonu ve immobilizasyon süresi döngüsel voltametri (CV) tekniği kullanılarak optimize edilirken, curcumin konsantrasyonu optimizasyonunda diferansiyel puls voltammetri (DPV) tekniği kullanılmıştır. Kurkumin için tayin limiti 0,29 µg/mL (0,78 µM) olarak hesaplanmıştır. Ayrıca, kurkumin-DNA etkileşimi farklı sürelerde gerçekleştirilerek, etkileşime süresinin yanıta etkisi elektrokimyasal empedans spektroskopisi (EIS) yöntemiyle incelenmiştir. Bu çalışmada sunulan elektrokimyasal yöntem, verimli ve hızlı analizi mümkün kılan kullanıcı dostu bir yöntem olmakla birlikte, DNA'nın küçük moleküllerle etkileşiminin araştırılması için umut verici bir araçtır.

Keywords

• Kitosan-altın kompoziti
• iyonik sıvı
• kurkumin
• elektrokimyasal DNA biyosensörü

Exploring the Interaction Between Curcumin and DNA using Chitosan-Gold Composite and Ionic Liquid Modified Disposable Electrodes

Abstract

A combination of chitosan (CHIT), gold composite, and ionic liquid (IL) modification was employed to construct novel pencil graphite electrodes (PGE) for the electrochemical investigation of curcumin-nucleic acid interaction. The electrochemical behaviour of these CHIT-Au-IL modified electrodes (CHIT-Au-IL-PGEs) was evaluated using techniques such as cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS). Additionally, scanning electron microscopy (SEM) was utilized to analyse the surface morphology and structural features of the electrodes. Optimization studies on the interaction between curcumin and nucleic acids were conducted using electrochemical techniques. DNA concentration and immobilization time on the CHIT-Au-IL-PGE surface were optimized using cyclic voltammetry (CV), while curcumin concentration was investigated through differential pulse voltammetry (DPV). The detection limit for curcumin was determined to be 0.29 µg/mL (0.78 µM). The curcumin-DNA interaction was further analysed at varying time intervals using EIS. The proposed electrochemical method is efficient, user-friendly, and rapid, providing a promising tool for screening small molecule interactions with DNA.

Keywords

• Chitosan-gold composite
• ionic liquid
• curcumin
• electrochemical DNA biosensor

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