A sensitive and label-free electrochemical impedance immunosensor for CDH 22 biomarker detection based on organo-functional silane modified ITO electrode

Abstract

Bu çalışmada, anti-CDH 22 antikoru ve CDH 22 antijeni arasındaki spesifik immüno-reaksiyona dayanan, kadherin benzeri protein (CDH 22) biyobelirtecinin tespiti için yeni bir elektrokimyasal etiketsiz immünosensör üretilmiştir. Geliştirilen immünosensör, anti-CDH 22 antikorlarının 3-siyanopropiltrimetoksisilan (CPTMS) ile modifiye edilmiş ITO substratı üzerinde immobilizasyonu ile oluşturulmuştur. Anti-CDH 22 antikorlarının CPTMS silanizasyon ajanına etkili bağlanması, morfolojik karakterizasyon (Taramalı Elektron Mikroskobu (SEM), Atomik Kuvvet Mikroskobu (AFM)) ve elektrokimyasal karakterizasyon (Elektrokimyasal İmpedans Spektroskopisi (EIS), Siklik Voltammetri (CV) kullanılarak incelendi. Optimum deney koşulları altında, ITO / CPTMS ile modifiye edilmiş elektrot, anti-CDH 22 antikor bağlanması için iyi bir bağlayıcıydır. Ek olarak, CPTMS modifiye elektrot CDH 22 antijen tespiti için etkili bir yüzey sunmuştur. İmmünosensör, geniş bir lineer tespit aralığı (0.03-3 pg / mL) ile düşük tespit limiti (9 fg / mL) ile sahipti. Ayrıca, iyi tekrarlanabilirlik, mükemmel tekrarlanabilirlik ve uzun depolama kararlılığına sahipti. Ek olarak, önerilen immünosensörün pratik uygulanabilirliği, insan serum numuneleri kullanılarak araştırıldı. İnsan serum numuneleri geri kazanım sonuçları (% 99,16 -% 101,94) önerilen biyosensörün doğruluğunu göstermektedir. Sonuç olarak, CPTMS, biyosensör yapımı için umut verici bir platform olabilir ve bu önerilen immünosensör gerçek insan serum analizi için uygulanabilir.

 

Keywords

• Cadherin-like protein 22,electrochemical impedance spectroscopy

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