Biosynthesis of Gold Nanoparticles using Scytosiphon lomentaria (Brown algae) and Spyridia filamentosa (Red algae) from Kyrenia Region and Evaluation of their Antimicrobial and Antioxidant Activity

Abstract

This study was carried out for biosynthesis of gold nanoparticles using Scytosiphon lomentaria (brown algae) and Spyridia filamentosa (red algae) and compared. Synthesized gold nanoparticles were characterized using the UV-Vis spectroscopy (UV-Vis), Fourier transform infrared (FTIR) and Master Sizer analysis. Antioxidant and antimicrobial activity of the synthesized nanoparticles were tested against E. coli and S. typhi. Macro algae involvement in the stabilization of the gold nanoparticles was confirmed by the presence of UV-Vis peak at 540 nm and is an indication of the presence of the gold nanoparticles (AuNPs). Stretching in peaks of the FTIR showed that the biomolecules present in the seaweed extract reduced the gold ions. Master sizer results for AuNPs were within the range of 15-55 nm. Antioxidant activity carried out using DPPH free radical scavenging activity revealed significant activity for both AuNPs. Biosynthesized AuNPs also showed antimicrobial activity against Salmonella typhii and Escherichia coli. The S. lomentaria gold nanoparticles exhibited great inhibition against Escherichia coli, whereas S. filamentosa gold nanoparticles showed great antibacterial activity against S .typhi. Synthesized AuNPs using S. lomentaria and S. filamentosa   as stabilizing agents showed convincing antioxidant and antimicrobial activity against gram negative and gram positive bacteria.

Keywords

• Gold nanoparticles,antimicrobial activity,Spyridia filamentosa,Scytosiphon lomentaria

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