The Effect of Interval Training Program on Nuclear Factor Erythroid-Derived 2-like 2 (NFE2L2/Nrf2) Gene Expression in Women

Year 2019, , 410 - 414, 01.12.2019

Neşe Akpınar

https://doi.org/10.15671/hjbc.546962

Cited By: 1

Abstract

Purpose in this
work, to investigate whether interval training
program has an effect on Nuclear factor
erythroid-derived 2-like 2 (NFE2L2/Nrf2)
gene expression in women.The research
was made on 12 women. Participants
were given a medium-term interval training program for 8 weeks, 3 days a week. The 
blood samples of the participants were collected before and after the 8
weeks of training. RNA isolation was performed using TRIzol Reagent from
peripheral blood mononuclear cells. NFE2L2 gene expression was
determined by Biomark Real-Time PCR (RT-PCR). The participants  was a significant increase in heart rate and
maximal oxygen use capacity (VO₂ max ) after the exercise (p
<0.001). There was a significant decrease in the body weight and body mass
index of women after the exercise (p <0.001). There was a decrease in NFE2L2
gene expressions after 8 weeks of the training program (p <0.05,). It shows that
interval exercise reduces NFE2L2 gene expression in women. 

Keywords

Exercise, gene expression, NFE2L2 gene, interval training

References

• 1. J.N.Stathopoulos , J.G.Tzanninis , A.A.Philippou,M. Koutsilieris. Epigenetic regulation on gene expression induced by physical exercise. JMusculoskelet Neuronal Interact.,13 (2013) 133-146.
• 2. P. Meier , M.Renga , H.Hoppeler, O.Baum The impact of antioxidant supplements and endurance exercise on genes of the carbohydrate and lipid metabolism in skeletal muscle of mice. Cell Biochem Funct., 31 (2013) 51–59.
• 3. G. Bangi , M.Delfabbro, C.Maurş, M.Corsi, G.Melegati.Haematological parameters in elite rugby players during a competitive season. Clin.Lab.Haem., 28 (2000) 183-188
• 4. Z. Rodak, J. Pucsuk, S. Boros, L. Josfai, A.W. Taylor. Changes in ürine 8-hytdroxydeoxyguanozine levels of super-marathon runners during a four-day race period. Life Sci.,66 ( 2000) 1763-1767.
• 5. Z.Rodak, T. Kaneko, S.Tahara , H. Nakamoto, H. Ohno, M. Sasvari, C. Nyakas, S.Goto. The effect of exercise training on oxidative damage of lipids, proteins, and DNA in rat skeletal muscle: evidence for beneficial outcomes. Free Radic Biol Med., 27 (1999) 69-74.
• 6. L.Packer, E.Cadenas, K.J.A Davies. Free radicals and exercise: an introduction.Free Radic. Biol. Med., 44 (2008) 123–125.
• 7. G. Gandhi, G.Gunjan. Exercise-Induced Genetic Damage: A Review. Int J Hum Genet., 9 (2009) 69-96
• 8. Z. Radak , Y.H. Chung , E. Koltai , A.W. Taylor, S. Goto. Exercise, oxidative stress and hormesis. Ageing Res. Rev., 7 ( 2007) 9:170.
• 9. M.C. Gomez-Cabrera, E. Domenech. J. Vina. Moderate exercise is an antioxidant: upregulation of antioxidant genes by training. Free Radic Biol Med., 44( 2008) 126-31.
• 10. C. L. Saw, Q. Wu, A.N. Kong. Anti-cancer and potential chemopreventive actions of ginseng by activating Nrf2 (NFE2L2) anti-oxidative stress/anti-inflammatory pathways, Chinese Medicine., 27( 2010) 5:37
• 11. C. Nilsson, S. Aboud, K. Karlen, B. Hejdeman, W. Urassa. Optimal blood mononuclear cell isolation procedures for gamma interferon enzyme-linked immunospot testing of healthy Swedish and Tanzanian subjects. Clin Vaccine Immunol., 15 ( 2008) 585–9.
• 12. F. Bayram , H. Diri , E. F Sener , M. Dundar , Y. Simsek. Genetic expressions of thrombophilic factors in patients with Sheehan’s syndrome. Gynecol Endocrinol., 32(11) (2016) 908–911
• 13. B. Jemiolo, Trappe S, Single muscle fiber gene expression in human skeletal muscle: validation of internal contro lwith exercise,Biochem.Biophys. Res. Commun., 320(3) (2004) 1043–1050.
• 14. M. Catoire , M. Mensink ,W.V. Boekschoten, M.Müller, P. Schrauwen, S.Kersten. Pronounced effects of acute endurance exercise on gene expression in resting and exercising human skeletal muscle, PloS One (2012) 7 (11) : e51066.
• 15. Z. Radak, S.Kumagai ,  A.W.Taylor , H. Hisashi, S.Goto. Effects of exercise on brain function: role of free radicals. Applied Physiology, Nutrition, and Metabolism., 32(2007) 942-946.
• 16. T.L Merry , M. Ristow . Nuclear factor erythroid-derived 2-like 2 (NFE2L2, Nrf2)mediates exercise-induced mitochondrial biogenesis and the anti-oxidant response in mice. The Journal of Physiology., 594(18) (2016) 5195–5207
• 17. K.Nakatani , M. Komatsu , T. Kato , T. Yamanaka , H. Takekura , et al. Habitual exercise induced resistance to oxidative stress. Free Radical Res., 39(9) (2005) 905-911,
• 18. H. Orhan , B.Van Holland ,B. Krab ,J. Moeken , N.P.Vermeulen , J.H. Meerman . Evaluation of a multiparameter biomarker set for oxidative damage in man: Increased urinary excretion of lipid, protein and DNA oxidation products after one hour of exercise. Free Radical Res, 38(12) (2004) 1269–1279
• 19. R.J. Bloomer , A.H. Goldfarb, J.M. Mckenzie. Oxidative stress response to aerobic exercise: Comparison of antioxidant supplements, Med Sci Sports Exerc.,38 ( 2006) 1099-1105
• 20. M. Horie, E. Warabi, S. Komine, S. Oh, J. Shoda, Cytoprotective role of Nrf2 in electrical pulse stimulated C2C12 myotube, PLoS One. , (2015) 10 (12) : e0144835
• 21. P. C.G Wang, Z. Li, D. Qi, S .Ding. Acute exercise stress promotes Ref/Nrf signaling and increases mitochondrial antioxidant activity in skeletal muscle, Exp. Physiol., 101(3) (2015) 410-20. 22. A.J. Done, M.J. Gage, N.C. Nieto, T. Traustadottir. Exercise-induced Nrf2-signaling is impaired in aging, Free Radical Biology and Medicine.,96 ( 2016) 130–138.
• 23. M.Narasimhan , J. Hong ,N. Atieno , V.R. Muthusamy, C.J. Davidson, N. Abu-Rmaileh, R. Richardson, A.V. Gomes, J.R. Hoidal, N.S.Rajasekaran. Nrf2 deficiency promotes apoptosis and impairs PAX7/MyoD expression in aging skeletal muscle cells, Free Radic. Biol. Med.,71 ( 2014) 402–414.
• 24. I. Bos , P. De Boever , L. Int Panis, S.Sarren, R. Meeusen. Negative effects of ultrafine particle exposure during forced exercise on the expression of Brain-Derived Neurotrophic Factor in the hippocampus of rats, Neuroscience., 223 ( 2012) 131–139.
• 25. T. Li ,S. He , S.Liu , Z. Kong , J. Wang , Y. Zhang. Effects of different exercise durations on Keap1-Nrf2-ARE pathway activation in mouse skeletal muscle, Free Radic. Res.,49(10) ( 2015) 1269–1274.
• 26. H.A. Scott , J.R. Latham , R. Callister . J.J. Pretto, K. Baines, N. Saltos, J.W. Uphan, L.G. Wood. Acute exercise is associated with reduced exhaled nitric oxide in physically inactive adults with asthma, Ann. Allergy Asthma Immunol., 114(6)( 2015) 470–479.
• 27. F.C. Gomes , L.G. Chuffa , W.R. Scarano ,P.F.F. Pinheiro, W.J.Favaro, R.F. Domeniconi. Nandrolone decanoate and resistance exercise training favor the occurrence of lesions and activate the inflammatory response in the ventral prostate. Andrology., 4 (2016) 473–480.
• 28. J. Done Aaron , T Tinna. Nrf2 mediates redox adaptations to exercise. Redox Biology.,10 ( 2016) 191–199