Bazı Akrep Türlerinden Elde Edilen Hemolenf ve Zehrin Antimikrobiyal Aktivitesi

Abstract

Bu çalışmanın amacı, bazı akrep türlerinden elde edilen hemolenf ve zehirlerin antibakteriyal ve antifungal özelliklerinin belirlenmesidir. Zehir örnekleri elektrik stimülasyonu yöntemiyle beş farklı akrep türünden elde edilmiştir. Bununla birlikte hemolenf sıvısı da beş farklı akrep türünden elde edilmiştir. 1:1 oranında tuz solüsyonu ile seyreltilen hemolenf ve zehirlerin antimikrobiyal aktiviteleri yedi bakteri ve üç mayadan oluşan 10 mikroorganizmaya karşı disk difüzyon yöntemi ile test edilmiştir. Protoiurus kraepelini’den elde edilen zehir örnekleri test edilen bütün mikroorganizmalar üzerinde 9-20 mm inhibisyon zonu meydana getirmiştir. Bu türden elde edilen zehir Bacillus subtilis’e karşı en yüksek antibakteriyal aktivite gösteren örnek olmuştur. Mesobuthus gibbosus’dan alınan zehir örnekleri Enterococcus faecalis ve Micrococcus luteus haricindeki tüm mikroorganizmalarda etkili (7-12 mm) olmuştur. Ayrıca, Androctonus crassicauda, Hottentotta saulcyi ve Leiurus abdullahbayrami’den elde edilen zehirler farklı düzeylerde antibakteriyal etkiye sahip olmakla birlikte Candida türleri üzerinde antifungal bir etkisi olmamıştır. Akrep hemolenflerinin Gram-pozitif ve Gram-negatif bakteriler üzerinde benzer etkilerinin olduğu gösterilmiştir. Bunun yanında, elde edilen hemolenflerin mikroorganizmalar üzerinde düşük etkiye (7-9 mm) sahip olduğu tespit edilmiştir

Keywords

• Akrep
• antimikrobiyal aktivite
• zehir
• hemolenf

Antimicrobial Activity of Hemolymph and Venom Obtained from Some Scorpion Species

Abstract

The aim of this study was to determine the antibacterial and antifungal activities of hemolymph and venom obtained from some scorpion species. Venom samples were obtained by electrical stimulation method from five different scorpion species. Again, hemolymph fluids were obtained from five different scorpion species. Antimicrobial activities of hemolymph and venom diluted 1:1 with sterile saline were tested against a total of 10 microorganisms including seven bacteria and three yeasts using disc diffusion method. Venom samples of Protoiurus kraepelini showed inhibition zones of 9-20 mm on all microorganisms tested. Venom from this species has the highest antibacterial activity against Bacillus subtilis. The venom samples of Mesobuthus gibbosus were effective (7-12 mm) on all microorganisms except Enterococcus faecalis and Micrococcus luteus. In addition, it was found that venoms obtained from Androctonus crassicauda, Hottentotta saulcyi and Leiurus abdullahbayrami have different levels of antibacterial effect but no antifungal effect against Candida species. Scorpion hemolymphs have been shown to have similar effects against Gram-positive and Gram-negative bacteria. Besides, the obtained hemolymphs have been found to have low effect (7-9 mm) on microorganisms.

Keywords

• Scorpion
• antimicrobial activity
• venom

References

1. Z. Zhao, Y. Ma, C. Dai, R. Zhao, S. Li, Y. Wu, Z. Cao, W. Li, Imcroporin, a new cationic antimicrobial peptide from the venom of the scorpion Isometrus maculates, Antimic. Agents & Chem., 53 (8) (2009) 3472-3477.
2. R. Singh, H.S. Nalwa, Medical applications of nanoparticles in biological imaging, cell labeling, antimicrobial agents, and anticancer nanodrugs, J. Biomed. Nanotech., 7 (4) (2011) 489-503.
3. N. Yiğit, Örümcek zehirlerinin antimikrobiyal aktivitesi, Mikrobiyo. Derg., 1 (3) (2003) 1-9.
4. R. Samy, P. Gopalakrishnakone, M.M. Thwin, T.K.V. H. Chow, Bow, E.H. Yap, T.W.J. Thong, Antibacterial activity of snake, scorpion and bee venoms: a comparison with purified venom phospholipase A2 enzymes, J. App. Microbiol., 102 (3) (2007) 650-659.
5. R.H. Qi, Y. Chen, Z.L. Guo, F. Zhang, Z. Fang, K. Huang, , H. Yu, Y.P. Wang, Identification and characterization of two novel cathelicidins from the frog Odorrana livida, Zool. Res., 40 (2) (2019) 94.
6. H. Steiner, D. Hultmark, Å. Engström, H. Bennich, H.G. Boman, Sequence and specificity of two antibacterial proteins involved in insect immunity, Nature, 292 (5820) (1981) 246.
7. L. Kuhn-Nentwig, Antimicrobial and cytolytic peptides of venomous arthropods, CMLS, 60 (12) (2003) 2651-2668.
8. Y. Ma, R. Zhao, Y. He, S. Li, J. Liu, Y. Wu, Z. Cao, W. Li, Transcriptome analysis of the venom gland of the scorpion Scorpiops jendeki: implication for the evolution of the scorpion venom arsenal, BMC, 10 (1) (2009) 290.

9. C.V.F. Batista, S.A. Román-González, S.P. Salas-Castillo, F.Z. Zamudio, F. Gómez-Lagunas, L.D. Possani, Proteomic analysis of the venom from the scorpion Tityus stigmurus: biochemical and physiological comparison with other Tityus species. Comp. Biochem. & Physiol Part C, 146 (1-2) (2007) 147-157.
10. H.M. Akef, Anticancer and antimicrobial activities of scorpion venoms and their peptides, Toxin Reviews, 38 (1) (2019) 41-53.
11. E. Zlotkin, A.S. Shulov, A simple device for collecting scorpion venom, Toxicon, 7 (4) (1969) 331-332.
12. A.W. Bauer, W.M.M. Kirby, J.C. Sherris, M. Turck, Antibiotic susceptibility testing by a standardized single disk method, Am. J. Clin. Path., 45 (4) (1966) 493-496.
13. A. Díaz-García, L. Morier-Díaz, Y. Frión-Herrera, H. Rodríguez-Sánchez, Y. Caballero-Lorenzo, D. Mendoza-Llanes, J.A. Fraga-Castro, In vitro anticancer effect of venom from Cuban scorpion Rhopalurus junceus against a panel of human cancer cell lines, J. Ven. Res., 4 (2013) 5.
14. Y. Guo, S.L. Liu, W.Z. Ju, C.Y. Li, P. Cao, Analgesic-antitumor peptide induces apoptosis and inhibits the proliferation of SW480 human colon cancer cells, Oncol. Let., 5 (2) (2013) 483-488.
15. A.K. Al Asmari, A.Q. Khan, Investigation of in vivo potential of scorpion venom against skin tumorigenesis in mice via targeting markers associated with cancer development, Drug Des. Dev. Ther., 10 (2016) 3387.
16. H. Akef, N. Kotb, D. Abo-Elmatty, S. Salem, Anti-proliferative effects of Androctonus amoreuxi Scorpion and Cerastes cerastes snake venoms on human prostate cancer cells, J. Cancer Prev., 22 (1) (2017) 40.
17. A. Almaaytah, M. Zhou, L. Wang, T. Chen, B. Walker, C. Shaw, Antimicrobial/cytolytic peptides from the venom of the North African scorpion, Androctonus amoreuxi: biochemical and functional characterization of natural peptides and a single site-substituted analog, Peptides, 35 (2) (2012) 291-299.
18. L. Cao, C. Dai, Z. Li, Z. Fan, Y. Song, Y. Wu, W. Li, Antibacterial activity and mechanism of a scorpion venom peptide derivative in vitro and in vivo, PloS one, 7 (7) (2012) e40135.
19. S. Ramírez-Carreto, V. Quintero-Hernández, J.M. Jiménez-Vargas, G. Corzo, L.D. Possani, B. Becerril, E. Ortiz, Gene cloning and functional characterization of four novel antimicrobial-like peptides from scorpions of the family Vaejovidae, Peptides, 34 (2) (2012) 290-295.
20. T. Arpornsuwan, W. Sriwai, J. Jaresitthikunchai, N. Phaonakrop, H. Sritanaudomchai, S. Roytrakul, Anticancer activities of antimicrobial BmKn2 peptides against oral and colon cancer cells, Int. J. Pept. Res. Ther., 20 (4) (2014) 501-509.
21. A. Bao, J. Zhong, X.C. Zeng, Y. Nie, L. Zhang, Z.F. Peng, A novel cysteine‐free venom peptide with strong antimicrobial activity against antibiotics‐resistant pathogens from the scorpion Opistophthalmus glabrifrons, J. Peptide Sci., 21 (10) (2015) 758-764.
22. U. Ahmed, N.K. Malik Mujaddad-ur-Rehman, S.A. Fawad, A. Fatima, Antibacterial activity of the venom of Heterometrus xanthopus, Ind. J. Pharm., 44 (4) (2012) 509.
23. W. Hong, R. Zhang, Z. Di, Y. He, Z. Zhao, J. Hu, Y. Wu, W. Li, Z. Cao, Design of histidine-rich peptides with enhanced bioavailability and inhibitory activity against hepatitis C virus, Biomaterials, 34 (13) (2013) 3511-3522.
24. W. Hong, T. Li, Y. Song, R. Zhang, Z. Zeng, S. Han, X. Zhang, Y. Wu, W. Li, Z. Cao, Inhibitory activity and mechanism of two scorpion venom peptides against herpes simplex virus type 1, Antivir. Res., 102 (2014) 1-10.
25. A.M. El-Bitar, M.M. Sarhan, C. Aoki, Y. Takahara, M. Komoto, L. Deng, M.A. Moustafa, H. Hotta, Virocidal activity of Egyptian scorpion venoms against hepatitis C virus, Virol. J., 12 (1) (2015) 47.
26. R. Zabihollahi, K.P. Bagheri, Z. Keshavarz, F. Motevalli, G. Bahramali, S.D. Siadat, S.B. Momen, D. Shahbazzadeh, M.R. Aghasadeghi, Venom components of Iranian scorpion Hemiscorpius lepturus inhibit the growth and replication of human immunodeficiency virus 1 (HIV-1), Iran. Biomed. J., 20 (5) (2016) 259.
27. Zeng, Z., Zhang, Q., Hong, W., Xie, Y., Liu, Y., Li, W., Wu, Y., Cao, Z. A scorpion defensin BmKDfsin4 inhibits hepatitis B virus replication in vitro, Toxins, 8 (5) (2016) 124.