PURIFICATION OF OVALBUMIN FROM EGG WHITE USING MOLECULAR IMPRINTED CRYOGELS

Abstract

Son yıllarda, dünya çapında gıda alerjisi en önemli sorunlardan biridir. Ovalbumin yumurta akında bulunan ana alerjanlardan biridir. Bu çalışmada, ovalbumin baskılanmamış ve baskılanmış poli (hidroksietil metakrilat-metakrilik asit) poli(HEMA-MAA) kriyojeller moleküler baskılama yöntemi kullanılarak sentezlenmiştir. Ovalbumin baskılanmış kriyojellerin karakterizasyonundan sonra, akış hızı, pH etkisi, başlangıçtaki ovalbumin konsantrasyonu ve sıcaklık parametrelerinin etkisi incelenmiştir. Ovalbumin baskılanmış ve baskılanmamış kriyojellerin ovalbumine karşı seçiciliğinin belirlenmesi, yarışmacı moleküller olarak lizozim ve transferrin kullanılarak gerçekleştirilmiştir. Ayrıca ovalbumin baskılanmış kriyojellerin yeniden kullanılabilirlik deneyleri gerçekleştirilmiştir. Ovalbuminin saflığı, sodyum-dodesil sülfat poliakrilamid jel elektroforezi kullanılarak yumurta akından belirlenmiştir.

Keywords

• ovalbumin
• cryogel
• molecular imprinting
• purification

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