Preparation of Bentonite-Cysteine Bent-Cys micro-composite affinity sorbents for Ferritin adsorption

Nevra Öztürk; Ahmet Tabak; Sinan Akgöl; Lokman Uzun; Adil Denizli

Preparation of Bentonite-Cysteine Bent-Cys micro-composite affinity sorbents for Ferritin adsorption

Abstract

This study focused on developing bentonite-cysteine micro-composite affinity sorbents Bent-Cys 38-105 m for Ferritin adsorption from aqueous solutions. The pseudo-specific affinity ligand L-cysteine was immobilized by covalent binding onto the bentonite structure. X-ray diffraction and FTIR analysis of Bent-Cys composite affinity sorbents were performed. The specific surface 2 area of the bentonite and Bent-Cys micro-composite structures were found to be 33.0 and 22.8 m /g, respectively. Elemental analysis of immobilized L-cysteine for nitrogen was estimated to be 541.3 mol/g bentonite. The non-specific ferritin adsorption onto bentonite structure was negligible ca. 0.9 mg/g . Higher adsorption values up to 40.0 mg/g were obtained in which Bent-Cys micro-composite affinity sorbents were used from aqueous solutions. Adsorption studies were performed to evaluate the effect of initial concentration of ferritin, pH, temperature. The desorption characteristics of Bent-Cys micro-composite affinity sorbents were also studied. The maximum amount of ferritin adsorption from aqueous solution was observed at pH 4.0. Ferritin molecules could be repeatedly adsorbed and desorbed with the micro-composite affinity sorbent system without noticeable loss in the ferritin adsorption capacity.

Keywords

bentonite,Ferritin adsorption,Composite systems,Affinity chromatography,Cysteine

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Details

Primary Language

English

Subjects

-

Journal Section

-

Authors

Nevra Öztürk This is me

Ahmet Tabak This is me

Sinan Akgöl This is me

Lokman Uzun This is me

Adil Denizli This is me

Publication Date

January 1, 2007

Submission Date

-

Acceptance Date

-

Published in Issue

Year 2007 Volume: 35 Number: 1

IZ

https://izlik.org/JA29ZP96HN

Cite

RIS / Bibtex

APA

Öztürk, N., Tabak, A., Akgöl, S., Uzun, L., & Denizli, A. (2007). Preparation of Bentonite-Cysteine Bent-Cys micro-composite affinity sorbents for Ferritin adsorption. Hacettepe Journal of Biology and Chemistry, 35(1), 67-76. https://izlik.org/JA29ZP96HN

AMA

1.Öztürk N, Tabak A, Akgöl S, Uzun L, Denizli A. Preparation of Bentonite-Cysteine Bent-Cys micro-composite affinity sorbents for Ferritin adsorption. HJBC. 2007;35(1):67-76. https://izlik.org/JA29ZP96HN

Chicago

Öztürk, Nevra, Ahmet Tabak, Sinan Akgöl, Lokman Uzun, and Adil Denizli. 2007. “Preparation of Bentonite-Cysteine Bent-Cys Micro-Composite Affinity Sorbents for Ferritin Adsorption”. Hacettepe Journal of Biology and Chemistry 35 (1): 67-76. https://izlik.org/JA29ZP96HN.

EndNote

Öztürk N, Tabak A, Akgöl S, Uzun L, Denizli A (January 1, 2007) Preparation of Bentonite-Cysteine Bent-Cys micro-composite affinity sorbents for Ferritin adsorption. Hacettepe Journal of Biology and Chemistry 35 1 67–76.

IEEE

[1]N. Öztürk, A. Tabak, S. Akgöl, L. Uzun, and A. Denizli, “Preparation of Bentonite-Cysteine Bent-Cys micro-composite affinity sorbents for Ferritin adsorption”, HJBC, vol. 35, no. 1, pp. 67–76, Jan. 2007, [Online]. Available: https://izlik.org/JA29ZP96HN

ISNAD

Öztürk, Nevra - Tabak, Ahmet - Akgöl, Sinan - Uzun, Lokman - Denizli, Adil. “Preparation of Bentonite-Cysteine Bent-Cys Micro-Composite Affinity Sorbents for Ferritin Adsorption”. Hacettepe Journal of Biology and Chemistry 35/1 (January 1, 2007): 67-76. https://izlik.org/JA29ZP96HN.

JAMA

1.Öztürk N, Tabak A, Akgöl S, Uzun L, Denizli A. Preparation of Bentonite-Cysteine Bent-Cys micro-composite affinity sorbents for Ferritin adsorption. HJBC. 2007;35:67–76.

MLA

Öztürk, Nevra, et al. “Preparation of Bentonite-Cysteine Bent-Cys Micro-Composite Affinity Sorbents for Ferritin Adsorption”. Hacettepe Journal of Biology and Chemistry, vol. 35, no. 1, Jan. 2007, pp. 67-76, https://izlik.org/JA29ZP96HN.

Vancouver

1.Nevra Öztürk, Ahmet Tabak, Sinan Akgöl, Lokman Uzun, Adil Denizli. Preparation of Bentonite-Cysteine Bent-Cys micro-composite affinity sorbents for Ferritin adsorption. HJBC [Internet]. 2007 Jan. 1;35(1):67-76. Available from: https://izlik.org/JA29ZP96HN