In Vitro Shoot Regeneration of Oregano Origanum minutiflorum O. Schwarz & Davis

Volume: 35 Number: 2 April 1, 2007
  • Dudu Özkum

In Vitro Shoot Regeneration of Oregano Origanum minutiflorum O. Schwarz & Davis

Abstract

In vitro shoot regeneration procedure for oregano O. minutiflorum O. Schwarz & Davis , endemic species of Turkey,is described. Leaf segments and shoot explants hypocotyl, single nodal segment and shoot tip excised from 30-40d old in vitro germinated seedlings and cultured on Murashige and Skoog MS and Gamborg B5 media containingdifferent combinations of benzyl amino purine BAP 0.0, 1.0, 2.0 or 3.0 mg L-1 and naphtalene acetic acid NAA 0.0, 0.1 or 0.5 mg L-1 . The single nodal segments were the most succesful explant in all hormone combinationsused. MS medium supplemented with 2.0 mg. L-1BAP + 0.1 mg L-1NAA was the most effective medium for shootformation. However for all explants, B5 medium with all phytohormone combinations had no effective success andtherefore were not suitable on in vitro shoot regeneration

Keywords

References

  1. 1. Murashige, T. and Skoog, F. A revised medium for rapid growth and bioassays with tobacco tissue cultures, Physiol. Plantarum, 15, 473- 497, 1962.
  2. 2. Gamborg, O.L., Miller, R.A. and Ojima, K. Nutrient requirements of suspension cultures of soybean root cells. Exp. Cell Res., 50, 151- 158, 1968.
  3. 3. Bajaj, Y.P.S, Furmanowa, M. and Olszowska, O. Biotechnology of the micropropagation of medicinal and aromatic plants. p. 60-103. In: Y.P.S. Bajaj (Ed.) Biotechnology in Agriculture and Forestry 4. Medicinal and Aromatic Plants I. Springer-Verlag, Berlin, 1988.
  4. 4. Ekim, T., Koyuncu, M., Vural, M., Duman, H., Aytaç, Z. and Adıgüzel, N. Türkiye Bitkileri Kırmızı Kitabı. Türkiye Tabiatını Koruma Derneği, VanYüzüncü Yıl Üniv. Ankara, 2000.
  5. 5. IUCN. Red List Categories: Version 3.1. Prepared By IUCN Species Survival Commission, IUCN, Gland Switzerland and Cambridge, UK, 2001.
  6. 6. Kumari, N. and Saradhi, P.P. Regeneration of plants from callus cultures of Origanum vulgare L. Plant Cell Rep., 11 (9), 476-479, 1992.
  7. 7. Crutis, O.F. and Shetty, K. Growth medium effects of vitrification, total phenolics, chlorophyll and water content of in vitro propagated oregano clones. Acta Hort., 426, 489-497, 1996.
  8. 8. Iyer, P.V. and Pai, J.S. In vitro regeneration of Majorana hortensis Moench from callus and nodal stem segments. Journal of Species and Aromatic Crops, 9 (1), 47-50, 2000.

Details

Primary Language

English

Subjects

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Journal Section

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Authors

Dudu Özkum This is me

Publication Date

April 1, 2007

Submission Date

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Acceptance Date

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Published in Issue

Year 2007 Volume: 35 Number: 2

APA
Özkum, D. (2007). In Vitro Shoot Regeneration of Oregano Origanum minutiflorum O. Schwarz & Davis. Hacettepe Journal of Biology and Chemistry, 35(2), 97-100. https://izlik.org/JA96ZX46TW
AMA
1.Özkum D. In Vitro Shoot Regeneration of Oregano Origanum minutiflorum O. Schwarz & Davis. HJBC. 2007;35(2):97-100. https://izlik.org/JA96ZX46TW
Chicago
Özkum, Dudu. 2007. “In Vitro Shoot Regeneration of Oregano Origanum Minutiflorum O. Schwarz & Davis”. Hacettepe Journal of Biology and Chemistry 35 (2): 97-100. https://izlik.org/JA96ZX46TW.
EndNote
Özkum D (April 1, 2007) In Vitro Shoot Regeneration of Oregano Origanum minutiflorum O. Schwarz & Davis. Hacettepe Journal of Biology and Chemistry 35 2 97–100.
IEEE
[1]D. Özkum, “In Vitro Shoot Regeneration of Oregano Origanum minutiflorum O. Schwarz & Davis”, HJBC, vol. 35, no. 2, pp. 97–100, Apr. 2007, [Online]. Available: https://izlik.org/JA96ZX46TW
ISNAD
Özkum, Dudu. “In Vitro Shoot Regeneration of Oregano Origanum Minutiflorum O. Schwarz & Davis”. Hacettepe Journal of Biology and Chemistry 35/2 (April 1, 2007): 97-100. https://izlik.org/JA96ZX46TW.
JAMA
1.Özkum D. In Vitro Shoot Regeneration of Oregano Origanum minutiflorum O. Schwarz & Davis. HJBC. 2007;35:97–100.
MLA
Özkum, Dudu. “In Vitro Shoot Regeneration of Oregano Origanum Minutiflorum O. Schwarz & Davis”. Hacettepe Journal of Biology and Chemistry, vol. 35, no. 2, Apr. 2007, pp. 97-100, https://izlik.org/JA96ZX46TW.
Vancouver
1.Dudu Özkum. In Vitro Shoot Regeneration of Oregano Origanum minutiflorum O. Schwarz & Davis. HJBC [Internet]. 2007 Apr. 1;35(2):97-100. Available from: https://izlik.org/JA96ZX46TW

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