Supermacroporous Support Having Dye for Reuseable Enzyme Immobilization

M. Nalan Tüzmen; Nilay Bereli; Müge Andaç; Sinan Akgöl; Adil Denizli

Supermacroporous Support Having Dye for Reuseable Enzyme Immobilization

Abstract

Poly acrylamide-allyl glycidyl ether [poly AAm-AGE ] cryogel was prepared by bulk polymerization which proceedsin an aqueous solution of monomers frozen inside a glass column cryo-polymerization . After thawing, the monolithiccryogel contains a continuous polymeric matrix having interconnected pores of 10-100 µm size. Cibacron Blue F3GAwas attached by covalent binding onto poly AAm-AGE cryogel via epoxy groups. Poly AAm-AGE cryogel wascharacterized by FTIR and scanning electron microscopy SEM . The equilibrium swelling degree of the poly AAmAGE monolithic cryogel was 6.84 g H2O/g cryogel. Poly AAm-AGE cryogel containing 68.9 µmol Cibacron BlueF3GA/g was used in the adsorption ofβ-casein from aqueous solution. The effects of initial concentration, pH, ionicstrength and flow rate on the adsorption efficiency of cryogel were studied in a column system. The non-specificadsorption ofβ-casein was very low 1.26 mg/g polymer . The maximum amount of β-casein adsorption from aqueoussolution in acetate buffer was 44.7 mg/g at pH 4.0. Up to 99% of the adsorbedβ-casein was eluted when 0.1 MTris/HCl buffer containing 0.5 M NaCl was used as elution agent. We concluded that Cibacron Blue F3GA attachedpoly AAm-AGE cryogel could be repeatedly applied forβ-casein adsorption without significant losses in the adsorptioncapacity

Keywords

β-Casein,cryogels,Dye-affinity Chromatography,Protein Purification

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Details

Primary Language

English

Subjects

-

Journal Section

-

Authors

M. Nalan Tüzmen This is me

Nilay Bereli This is me

Müge Andaç This is me

Sinan Akgöl This is me

Adil Denizli This is me

Publication Date

January 1, 2008

Submission Date

-

Acceptance Date

-

Published in Issue

Year 2008 Volume: 36 Number: 1

IZ

https://izlik.org/JA34HX26BK

Cite

RIS / Bibtex

APA

Tüzmen, M. N., Bereli, N., Andaç, M., Akgöl, S., & Denizli, A. (2008). Supermacroporous Support Having Dye for Reuseable Enzyme Immobilization. Hacettepe Journal of Biology and Chemistry, 36(1), 9-19. https://izlik.org/JA34HX26BK

AMA

1.Tüzmen MN, Bereli N, Andaç M, Akgöl S, Denizli A. Supermacroporous Support Having Dye for Reuseable Enzyme Immobilization. HJBC. 2008;36(1):9-19. https://izlik.org/JA34HX26BK

Chicago

Tüzmen, M. Nalan, Nilay Bereli, Müge Andaç, Sinan Akgöl, and Adil Denizli. 2008. “Supermacroporous Support Having Dye for Reuseable Enzyme Immobilization”. Hacettepe Journal of Biology and Chemistry 36 (1): 9-19. https://izlik.org/JA34HX26BK.

EndNote

Tüzmen MN, Bereli N, Andaç M, Akgöl S, Denizli A (January 1, 2008) Supermacroporous Support Having Dye for Reuseable Enzyme Immobilization. Hacettepe Journal of Biology and Chemistry 36 1 9–19.

IEEE

[1]M. N. Tüzmen, N. Bereli, M. Andaç, S. Akgöl, and A. Denizli, “Supermacroporous Support Having Dye for Reuseable Enzyme Immobilization”, HJBC, vol. 36, no. 1, pp. 9–19, Jan. 2008, [Online]. Available: https://izlik.org/JA34HX26BK

ISNAD

Tüzmen, M. Nalan - Bereli, Nilay - Andaç, Müge - Akgöl, Sinan - Denizli, Adil. “Supermacroporous Support Having Dye for Reuseable Enzyme Immobilization”. Hacettepe Journal of Biology and Chemistry 36/1 (January 1, 2008): 9-19. https://izlik.org/JA34HX26BK.

JAMA

1.Tüzmen MN, Bereli N, Andaç M, Akgöl S, Denizli A. Supermacroporous Support Having Dye for Reuseable Enzyme Immobilization. HJBC. 2008;36:9–19.

MLA

Tüzmen, M. Nalan, et al. “Supermacroporous Support Having Dye for Reuseable Enzyme Immobilization”. Hacettepe Journal of Biology and Chemistry, vol. 36, no. 1, Jan. 2008, pp. 9-19, https://izlik.org/JA34HX26BK.

Vancouver

1.M. Nalan Tüzmen, Nilay Bereli, Müge Andaç, Sinan Akgöl, Adil Denizli. Supermacroporous Support Having Dye for Reuseable Enzyme Immobilization. HJBC [Internet]. 2008 Jan. 1;36(1):9-19. Available from: https://izlik.org/JA34HX26BK