Quartz Crystal Piezo Electrode Surface Modification for Gold Nano-particles Immobilization

Year 2007, Volume: 35 Issue: 3, 203 - 208, 01.08.2007

Fatma Ayhan Aydan Gülsu Hakan Ayhan

https://izlik.org/JA98RM85XT

Abstract

In this study silver electrode quartz crystal surfaces were cleaned and modified to immobilize gold nanoparticles.First, the crystal surfaces were cleaned and activated with sodium hydroxide, acetone and methanol. After the cleaningand the activation process, first cysteamine immobilization stage was performed in phosphate buffer pH=7.0 .Glutaraldehyde was used as a spacer arm and immobilized on the electrode surfaces in tetraborate buffer pH 8.2 .After then, second cysteamine immobilization step was achieved. Frequency shift were measured in all of themodification steps. Gold nanoparticles were immobilized on the modified surfaces by self-assembled monolayer SAM formation, for expanded crystal surface

Keywords

Surface modification , self-assembled monolayer , gold nanoparticles Immobilization

References

• Shubo, H.A., three-dimensional heterogeneous DNA sensing surface formed by attaching oligodeoxynucleotide-capped gold nanoparticles onto a gold coated quartz crystal, Chem. Commun., (2001) 609 .
• Zhou, X.C., Microgravimetric DNA sensor based on quartz crystal microbalance: comparison of oligonucleotide immobilization methods and the application in genetic diagnosis, Biosensors and Bioelectronics, 16 (2001) 85.
• Göpel, W. Bioelectronics and nanotechnologies, Biosensors and Bioelectronics, 13 (1998) 723.
• DeSilva, A. P. Gunaratne, H. Q. N. McCoy, C. P., A molecular photoionic and gate based on fluorescent signalling, Nature, 364 (1993) 42.
• Andrew N. Shipway, Nanoparticle Arrays on Surfaces for Elektronic, Optical and sensor applications, CHEMPHYSCHEM, 1 (2000) 18.
• Csáki, A. DNA monolayer on gold substrates characterized by nanoparticle labeling and scanning force microscopy, Nucleic Acids Research, 29 (2001) 16.
• Kimura, K. and Takashima, S., Molecular Approach to the Surface Potential Estimate of Thiolate- Modified Gold Nanoparticles, J. Phys. Chem. B, 106 (2002) 7260.
• Lvov, Y., Munge, B., Giraldo, O., Ichinose, I., Suib, S.L., Rusling, J.F., Films of manganese oxide nanoparticles with polycations or myoglobin from alternate-layer adsorption. Langmuir, 16 (2000) Tabrizi, A., Ayhan, F., Ayhan, H., Gold
• Nanoparticle Synthesis and Characterisation For self-assembled monolayer Immobilization on to Piezo Crystal surfaces, Journal of Biomaterial Science, Polymer Edition, (2008), submitted.
• Koçum, C., Erdamar, A., Ayhan, H., Design of Temperature Controlled Quartz Crystal Microbalance System, Review of Scientifi Instruments, (2008) submitted.
• Sauerbrey, G., Verwendung von Schwingquarzen zur dunner Schichten und zur. Z Phys., 155 (1959) Tonya M. Herne and Michael J. Tarlov
• Characterization of DNA Probes Immobilized on Gold Surfaces, J. Am. Chem. Soc., 119 (1997)
• Wang, J., Flow injection amperometric detection of OP nerve agents based on an organophosphorus– hydrolase biosensor detector, Biosensors and Bioelectronics 18 (2003) 255.