Boronic Acid Functionalized Adsorbents for Antibody Removal

Başak Şaşmaz; Gözde Baydemir; Nilay Bereli; Serap Şenel; Handan Yavuz

Boronic Acid Functionalized Adsorbents for Antibody Removal

Abstract

In this study a novel approach was developed to obtain an efficient separation of human-IgG from human plasma. 4- Vinylphenylboronic acid VPBA and 2-hydroxyethylmethacrylate HEMA were chosen as monomers. Spherical microbeads with an average size of 100-140 µm were obtained by the radical suspension polymerization of VPBA and HEMA conducted in an aqueous dispersion medium. VPBA monomer was characterized by FTIR. p HEMA-VPBA beads were characterized by swelling studies, FTIR and SEM. The pHEMA and p HEMA-VPBA microbeads were contacted with blood in in-vitro systems. Loss of the blood cells and the clotting times were also followed. Good blood compatibility properties of both pHEMA and p HEMA-VPBA were clearly observed with blood coagulation experiments. Loss of cells in the blood contacting with pHEMA and p HEMA-VPBA microbeads were nearly the same. These VPBA containing p HEMA-VPBA microbeads with a swelling ratio of 57 % were used in the adsorption/desorption of human-IgG from aqueous solutions and human plasma. The maximum human-IgG adsorption 139 mg/g polymer was observed at pH 8.5 for HEPES buffer. IgG adsorption from human plasma was 38.75 mg/g. It was also observed that human-IgG could be repeatedly adsorbed and desorbed with p HEMA-VPBA microbeads without significant loss in the adsorption capacity.

Keywords

Boronic Acid,Antibody Removal,Boranate affinity

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Details

Primary Language

English

Subjects

-

Journal Section

-

Authors

Başak Şaşmaz This is me

Gözde Baydemir This is me

Nilay Bereli This is me

Serap Şenel This is me

Handan Yavuz This is me

Publication Date

January 1, 2008

Submission Date

-

Acceptance Date

-

Published in Issue

Year 2008 Volume: 36 Number: 1

IZ

https://izlik.org/JA64XS87TC

Cite

RIS / Bibtex

APA

Şaşmaz, B., Baydemir, G., Bereli, N., Şenel, S., & Yavuz, H. (2008). Boronic Acid Functionalized Adsorbents for Antibody Removal. Hacettepe Journal of Biology and Chemistry, 36(1), 47-60. https://izlik.org/JA64XS87TC

AMA

1.Şaşmaz B, Baydemir G, Bereli N, Şenel S, Yavuz H. Boronic Acid Functionalized Adsorbents for Antibody Removal. HJBC. 2008;36(1):47-60. https://izlik.org/JA64XS87TC

Chicago

Şaşmaz, Başak, Gözde Baydemir, Nilay Bereli, Serap Şenel, and Handan Yavuz. 2008. “Boronic Acid Functionalized Adsorbents for Antibody Removal”. Hacettepe Journal of Biology and Chemistry 36 (1): 47-60. https://izlik.org/JA64XS87TC.

EndNote

Şaşmaz B, Baydemir G, Bereli N, Şenel S, Yavuz H (January 1, 2008) Boronic Acid Functionalized Adsorbents for Antibody Removal. Hacettepe Journal of Biology and Chemistry 36 1 47–60.

IEEE

[1]B. Şaşmaz, G. Baydemir, N. Bereli, S. Şenel, and H. Yavuz, “Boronic Acid Functionalized Adsorbents for Antibody Removal”, HJBC, vol. 36, no. 1, pp. 47–60, Jan. 2008, [Online]. Available: https://izlik.org/JA64XS87TC

ISNAD

Şaşmaz, Başak - Baydemir, Gözde - Bereli, Nilay - Şenel, Serap - Yavuz, Handan. “Boronic Acid Functionalized Adsorbents for Antibody Removal”. Hacettepe Journal of Biology and Chemistry 36/1 (January 1, 2008): 47-60. https://izlik.org/JA64XS87TC.

JAMA

1.Şaşmaz B, Baydemir G, Bereli N, Şenel S, Yavuz H. Boronic Acid Functionalized Adsorbents for Antibody Removal. HJBC. 2008;36:47–60.

MLA

Şaşmaz, Başak, et al. “Boronic Acid Functionalized Adsorbents for Antibody Removal”. Hacettepe Journal of Biology and Chemistry, vol. 36, no. 1, Jan. 2008, pp. 47-60, https://izlik.org/JA64XS87TC.

Vancouver

1.Başak Şaşmaz, Gözde Baydemir, Nilay Bereli, Serap Şenel, Handan Yavuz. Boronic Acid Functionalized Adsorbents for Antibody Removal. HJBC [Internet]. 2008 Jan. 1;36(1):47-60. Available from: https://izlik.org/JA64XS87TC