Tyrosinase Immobilization on Cu2+Chelated Poly ethylene glycol dimethacrylate-N-Vinyl Imidazole Beads
Abstract
Poly ethylene glycol dimethacrylate-n-vinyl imidazole [poly EGDMA-VIM ] hydrogel beads average diameter 150–200 µm was prepared by copolymerizing of ethylene glycol dimethacrylate EGDMA with n-vinyl imidazole VIM .Poly EGDMA-VIM beads had a specific surface area of 59.8 m2/g. Poly- EGDMA-VIM beads were characterized byswelling studies, elemental and SEM. Cu2+ions were chelated on the poly EGDMA-VIM beads, then these beadswere used in the reversible adsorption of mushroom tyrosinase. The maximum tyrosinase adsorption capacity of thepoly EGDMA-VIM –Cu2+beads was observed as 14.04 mg/g at pH 6.5. Storage stability was found to increase withimmobilization. It was observed that enzyme could be repeatedly adsorbed and desorbed without significant loss inadsorption capacity or enzyme activity
Keywords
References
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Details
Primary Language
English
Subjects
-
Journal Section
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Publication Date
August 1, 2007
Submission Date
-
Acceptance Date
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Published in Issue
Year 2007 Volume: 35 Number: 3
APA
Osman, B., Kara, A., & Beşirli, N. (2007). Tyrosinase Immobilization on Cu2+Chelated Poly ethylene glycol dimethacrylate-N-Vinyl Imidazole Beads. Hacettepe Journal of Biology and Chemistry, 35(3), 233-241. https://izlik.org/JA36JR23KP
AMA
1.Osman B, Kara A, Beşirli N. Tyrosinase Immobilization on Cu2+Chelated Poly ethylene glycol dimethacrylate-N-Vinyl Imidazole Beads. HJBC. 2007;35(3):233-241. https://izlik.org/JA36JR23KP
Chicago
Osman, Bilgen, Ali Kara, and Necati Beşirli. 2007. “Tyrosinase Immobilization on Cu2+Chelated Poly Ethylene Glycol Dimethacrylate-N-Vinyl Imidazole Beads”. Hacettepe Journal of Biology and Chemistry 35 (3): 233-41. https://izlik.org/JA36JR23KP.
EndNote
Osman B, Kara A, Beşirli N (August 1, 2007) Tyrosinase Immobilization on Cu2+Chelated Poly ethylene glycol dimethacrylate-N-Vinyl Imidazole Beads. Hacettepe Journal of Biology and Chemistry 35 3 233–241.
IEEE
[1]B. Osman, A. Kara, and N. Beşirli, “Tyrosinase Immobilization on Cu2+Chelated Poly ethylene glycol dimethacrylate-N-Vinyl Imidazole Beads”, HJBC, vol. 35, no. 3, pp. 233–241, Aug. 2007, [Online]. Available: https://izlik.org/JA36JR23KP
ISNAD
Osman, Bilgen - Kara, Ali - Beşirli, Necati. “Tyrosinase Immobilization on Cu2+Chelated Poly Ethylene Glycol Dimethacrylate-N-Vinyl Imidazole Beads”. Hacettepe Journal of Biology and Chemistry 35/3 (August 1, 2007): 233-241. https://izlik.org/JA36JR23KP.
JAMA
1.Osman B, Kara A, Beşirli N. Tyrosinase Immobilization on Cu2+Chelated Poly ethylene glycol dimethacrylate-N-Vinyl Imidazole Beads. HJBC. 2007;35:233–241.
MLA
Osman, Bilgen, et al. “Tyrosinase Immobilization on Cu2+Chelated Poly Ethylene Glycol Dimethacrylate-N-Vinyl Imidazole Beads”. Hacettepe Journal of Biology and Chemistry, vol. 35, no. 3, Aug. 2007, pp. 233-41, https://izlik.org/JA36JR23KP.
Vancouver
1.Bilgen Osman, Ali Kara, Necati Beşirli. Tyrosinase Immobilization on Cu2+Chelated Poly ethylene glycol dimethacrylate-N-Vinyl Imidazole Beads. HJBC [Internet]. 2007 Aug. 1;35(3):233-41. Available from: https://izlik.org/JA36JR23KP