Method Optimization and Separation of Aromatic Biogenic Amines and Precursor Amino Acids by High Performance Liquid Chromatography

Nurullah Şanlı; Senem Şanlı; Güleren Ozkan

Method Optimization and Separation of Aromatic Biogenic Amines and Precursor Amino Acids by High Performance Liquid Chromatography

Abstract

In this study, the determination of aromatic biogen amines; histamine, β-phenyl ethylamine, tyramine and tryptamine,and their precursor amino acids; histidine, phenylalanine, tyrosine and tyriptophan was performed without using anyderivatization reagents. Mobile phase was optimised by establishing relationships between retention parameters andReichardt’s ETNscale of solvent polarity. In addition, selectivity α and resolution RS values were used in order toestablish a general model relating elution behaviour of substances with the composition of mobile phase. In order tofind the optimum pH value, pKa values of amino acids in the mobile phase were determined by potentiometric methodin 20% and 30% v/v MeCN media. For biogenic amines and amino acids studied, the optimised mobile phase wascomposed of acetonitrile-water and 6.10-3mol l-11-heptanesulphonic acid sodium salt adjusted at pH 2.45 with a percentage of MeCN-water of 28% v/v

Keywords

Biogenic amines,LC,Optimisation of mobile phase,Reichardt’s ETN scale of solvent polarity

References

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Details

Primary Language

English

Subjects

-

Journal Section

-

Authors

Nurullah Şanlı This is me

Senem Şanlı This is me

Güleren Ozkan This is me

Publication Date

April 1, 2007

Submission Date

-

Acceptance Date

-

Published in Issue

Year 2007 Volume: 35 Number: 2

IZ

https://izlik.org/JA55RP59TF

Cite

RIS / Bibtex

APA

Şanlı, N., Şanlı, S., & Ozkan, G. (2007). Method Optimization and Separation of Aromatic Biogenic Amines and Precursor Amino Acids by High Performance Liquid Chromatography. Hacettepe Journal of Biology and Chemistry, 35(2), 129-134. https://izlik.org/JA55RP59TF

AMA

1.Şanlı N, Şanlı S, Ozkan G. Method Optimization and Separation of Aromatic Biogenic Amines and Precursor Amino Acids by High Performance Liquid Chromatography. HJBC. 2007;35(2):129-134. https://izlik.org/JA55RP59TF

Chicago

Şanlı, Nurullah, Senem Şanlı, and Güleren Ozkan. 2007. “Method Optimization and Separation of Aromatic Biogenic Amines and Precursor Amino Acids by High Performance Liquid Chromatography”. Hacettepe Journal of Biology and Chemistry 35 (2): 129-34. https://izlik.org/JA55RP59TF.

EndNote

Şanlı N, Şanlı S, Ozkan G (April 1, 2007) Method Optimization and Separation of Aromatic Biogenic Amines and Precursor Amino Acids by High Performance Liquid Chromatography. Hacettepe Journal of Biology and Chemistry 35 2 129–134.

IEEE

[1]N. Şanlı, S. Şanlı, and G. Ozkan, “Method Optimization and Separation of Aromatic Biogenic Amines and Precursor Amino Acids by High Performance Liquid Chromatography”, HJBC, vol. 35, no. 2, pp. 129–134, Apr. 2007, [Online]. Available: https://izlik.org/JA55RP59TF

ISNAD

Şanlı, Nurullah - Şanlı, Senem - Ozkan, Güleren. “Method Optimization and Separation of Aromatic Biogenic Amines and Precursor Amino Acids by High Performance Liquid Chromatography”. Hacettepe Journal of Biology and Chemistry 35/2 (April 1, 2007): 129-134. https://izlik.org/JA55RP59TF.

JAMA

1.Şanlı N, Şanlı S, Ozkan G. Method Optimization and Separation of Aromatic Biogenic Amines and Precursor Amino Acids by High Performance Liquid Chromatography. HJBC. 2007;35:129–134.

MLA

Şanlı, Nurullah, et al. “Method Optimization and Separation of Aromatic Biogenic Amines and Precursor Amino Acids by High Performance Liquid Chromatography”. Hacettepe Journal of Biology and Chemistry, vol. 35, no. 2, Apr. 2007, pp. 129-34, https://izlik.org/JA55RP59TF.

Vancouver

1.Nurullah Şanlı, Senem Şanlı, Güleren Ozkan. Method Optimization and Separation of Aromatic Biogenic Amines and Precursor Amino Acids by High Performance Liquid Chromatography. HJBC [Internet]. 2007 Apr. 1;35(2):129-34. Available from: https://izlik.org/JA55RP59TF