Synthesis and Characterization of Macroporous Poly Acrylamide-Methacrylamido Histidine Cryogels and Their Use in Antibody Purification

İlker Koç; Nilay Bereli; Gözde Baydemir; Igor Yu Galaev; Adil Denizli

Synthesis and Characterization of Macroporous Poly Acrylamide-Methacrylamido Histidine Cryogels and Their Use in Antibody Purification

Abstract

Poly acrylamide-methacrylamidohistidine [poly AAm-MAH ] cryogel was prepared by bulk polymerization whichproceeds in aqueous solution of monomers frozen inside a syringe column cryo-polymerization . After thawing, themonolithic cryogel contains a continuous polymeric matrix having interconnected pores of 10-100 µm size. Poly AAmMAH cryogel was characterized by swelling studies, FTIR and scanning electron microscopy. The equilibrium swellingdegree of the poly AAm-MAH monolithic cryogel was 6.97 g H2O/g cryogel. Poly AAm-MAH cryogel was used in theadsorption/desorption of immunoglobulin G IgG from aqueous solutions and human plasma. The non-specificadsorption of IgG was very low 0.22 mg/g . The maximum amount of IgG adsorption from aqueous solution in HEPESbuffer was 32.879 mg/g at pH 7.4. Higher IgG adsorption value was obtained from human plasma up to 100 mg/g .Desorption of IgG from poly AAm-MAH cryogel was obtained using 20 mM Acetate buffer containing 1.0 M NaCl atpH 4. It was observed that IgG could be repeatedly adsorbed and desorbed with poly AAm-MAH cryogel withoutsignificant loss in the adsorption capacity

Keywords

Affinity chromatography,cryogel,Protein Purification,IgG

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Details

Primary Language

English

Subjects

-

Journal Section

-

Authors

İlker Koç This is me

Nilay Bereli This is me

Gözde Baydemir This is me

Igor Yu Galaev This is me

Adil Denizli This is me

Publication Date

January 1, 2008

Submission Date

-

Acceptance Date

-

Published in Issue

Year 2008 Volume: 36 Number: 1

IZ

https://izlik.org/JA36DU22NX

Cite

RIS / Bibtex

APA

Koç, İ., Bereli, N., Baydemir, G., Galaev, I. Y., & Denizli, A. (2008). Synthesis and Characterization of Macroporous Poly Acrylamide-Methacrylamido Histidine Cryogels and Their Use in Antibody Purification. Hacettepe Journal of Biology and Chemistry, 36(1), 61-76. https://izlik.org/JA36DU22NX

AMA

1.Koç İ, Bereli N, Baydemir G, Galaev IY, Denizli A. Synthesis and Characterization of Macroporous Poly Acrylamide-Methacrylamido Histidine Cryogels and Their Use in Antibody Purification. HJBC. 2008;36(1):61-76. https://izlik.org/JA36DU22NX

Chicago

Koç, İlker, Nilay Bereli, Gözde Baydemir, Igor Yu Galaev, and Adil Denizli. 2008. “Synthesis and Characterization of Macroporous Poly Acrylamide-Methacrylamido Histidine Cryogels and Their Use in Antibody Purification”. Hacettepe Journal of Biology and Chemistry 36 (1): 61-76. https://izlik.org/JA36DU22NX.

EndNote

Koç İ, Bereli N, Baydemir G, Galaev IY, Denizli A (January 1, 2008) Synthesis and Characterization of Macroporous Poly Acrylamide-Methacrylamido Histidine Cryogels and Their Use in Antibody Purification. Hacettepe Journal of Biology and Chemistry 36 1 61–76.

IEEE

[1]İ. Koç, N. Bereli, G. Baydemir, I. Y. Galaev, and A. Denizli, “Synthesis and Characterization of Macroporous Poly Acrylamide-Methacrylamido Histidine Cryogels and Their Use in Antibody Purification”, HJBC, vol. 36, no. 1, pp. 61–76, Jan. 2008, [Online]. Available: https://izlik.org/JA36DU22NX

ISNAD

Koç, İlker - Bereli, Nilay - Baydemir, Gözde - Galaev, Igor Yu - Denizli, Adil. “Synthesis and Characterization of Macroporous Poly Acrylamide-Methacrylamido Histidine Cryogels and Their Use in Antibody Purification”. Hacettepe Journal of Biology and Chemistry 36/1 (January 1, 2008): 61-76. https://izlik.org/JA36DU22NX.

JAMA

1.Koç İ, Bereli N, Baydemir G, Galaev IY, Denizli A. Synthesis and Characterization of Macroporous Poly Acrylamide-Methacrylamido Histidine Cryogels and Their Use in Antibody Purification. HJBC. 2008;36:61–76.

MLA

Koç, İlker, et al. “Synthesis and Characterization of Macroporous Poly Acrylamide-Methacrylamido Histidine Cryogels and Their Use in Antibody Purification”. Hacettepe Journal of Biology and Chemistry, vol. 36, no. 1, Jan. 2008, pp. 61-76, https://izlik.org/JA36DU22NX.

Vancouver

1.İlker Koç, Nilay Bereli, Gözde Baydemir, Igor Yu Galaev, Adil Denizli. Synthesis and Characterization of Macroporous Poly Acrylamide-Methacrylamido Histidine Cryogels and Their Use in Antibody Purification. HJBC [Internet]. 2008 Jan. 1;36(1):61-76. Available from: https://izlik.org/JA36DU22NX