Preparation of Fe III -Chelated Poly HEMA-MAH Cryogel for Lysozyme Adsorption

Bahar Ergün; Ali Derazshamshir; Mehmet Odabaşı

Preparation of Fe III -Chelated Poly HEMA-MAH Cryogel for Lysozyme Adsorption

Abstract

Poly 2-hydroxyethyl methacrylate-N-methacryloyl- L -histidine methyl ester [poly HEMA-MAH ] cryogel was preparedby bulk polymerization which proceeds in an aqueous solution of monomers frozen inside a glass column cryo-polymerization . After thawing, the monolithic cryogel contains a continuous polymeric matrix having interconnected poresof 10-50 μm size. Then the monolithic cryogel was loaded with Fe III ions to form the metal chelate with poly HEMAMAH cryogel. Poly HEMA-MAH cryogel was characterized by swelling studies, FTIR, scanning electron microscopy,and elemental analysis. The equilibrium swelling degree of the poly HEMA-MAH monolithic cryogel was 2.82 g H2O/gcryogel. Poly HEMA-MAH cryogel containing 36.4 μmol MAH/g was used in the adsorption/desorption of lysozymefrom aqueous solutions. The nonspecific adsorption of lysozyme was very low 1.1 mg/g . The maximum amount oflysozyme adsorption from aqueous solution in phosphate buffer was 40.7 mg/g at pH 7.0. It was observed thatlysozyme could be repeatedly adsorbed and desorbed with the poly HEMA-MAH cyogel without significant loss ofadsorption capacity

Keywords

cryogel,lysozyme,immobilized metal affinity chromatography,histidine,poly HEMA

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Details

Primary Language

English

Subjects

-

Journal Section

-

Authors

Bahar Ergün This is me

Ali Derazshamshir This is me

Mehmet Odabaşı This is me

Publication Date

April 1, 2007

Submission Date

-

Acceptance Date

-

Published in Issue

Year 2007 Volume: 35 Number: 2

IZ

https://izlik.org/JA23TC46AA

Cite

RIS / Bibtex

APA

Ergün, B., Derazshamshir, A., & Odabaşı, M. (2007). Preparation of Fe III -Chelated Poly HEMA-MAH Cryogel for Lysozyme Adsorption. Hacettepe Journal of Biology and Chemistry, 35(2), 143-148. https://izlik.org/JA23TC46AA

AMA

1.Ergün B, Derazshamshir A, Odabaşı M. Preparation of Fe III -Chelated Poly HEMA-MAH Cryogel for Lysozyme Adsorption. HJBC. 2007;35(2):143-148. https://izlik.org/JA23TC46AA

Chicago

Ergün, Bahar, Ali Derazshamshir, and Mehmet Odabaşı. 2007. “Preparation of Fe III -Chelated Poly HEMA-MAH Cryogel for Lysozyme Adsorption”. Hacettepe Journal of Biology and Chemistry 35 (2): 143-48. https://izlik.org/JA23TC46AA.

EndNote

Ergün B, Derazshamshir A, Odabaşı M (April 1, 2007) Preparation of Fe III -Chelated Poly HEMA-MAH Cryogel for Lysozyme Adsorption. Hacettepe Journal of Biology and Chemistry 35 2 143–148.

IEEE

[1]B. Ergün, A. Derazshamshir, and M. Odabaşı, “Preparation of Fe III -Chelated Poly HEMA-MAH Cryogel for Lysozyme Adsorption”, HJBC, vol. 35, no. 2, pp. 143–148, Apr. 2007, [Online]. Available: https://izlik.org/JA23TC46AA

ISNAD

Ergün, Bahar - Derazshamshir, Ali - Odabaşı, Mehmet. “Preparation of Fe III -Chelated Poly HEMA-MAH Cryogel for Lysozyme Adsorption”. Hacettepe Journal of Biology and Chemistry 35/2 (April 1, 2007): 143-148. https://izlik.org/JA23TC46AA.

JAMA

1.Ergün B, Derazshamshir A, Odabaşı M. Preparation of Fe III -Chelated Poly HEMA-MAH Cryogel for Lysozyme Adsorption. HJBC. 2007;35:143–148.

MLA

Ergün, Bahar, et al. “Preparation of Fe III -Chelated Poly HEMA-MAH Cryogel for Lysozyme Adsorption”. Hacettepe Journal of Biology and Chemistry, vol. 35, no. 2, Apr. 2007, pp. 143-8, https://izlik.org/JA23TC46AA.

Vancouver

1.Bahar Ergün, Ali Derazshamshir, Mehmet Odabaşı. Preparation of Fe III -Chelated Poly HEMA-MAH Cryogel for Lysozyme Adsorption. HJBC [Internet]. 2007 Apr. 1;35(2):143-8. Available from: https://izlik.org/JA23TC46AA