Preparation and Characterization of Polyhydroxybutyrate Scaffolds to be Used in Tissue Engineering Applications

Eylem Öztürk Güven; Murat Demirbilek; Necdet Sağlam; Zeynep Karahaliloğlu; Ebru Erdal; Cem Bayram; Emir Baki Denkbaş

Preparation and Characterization of Polyhydroxybutyrate Scaffolds to be Used in Tissue Engineering Applications

Abstract

Polyhydroxyalkanoates PHA are good alternatives on account of biocompatible and biodegradable properties toproduce materials as scaffolds for engineered tissues. Polyhydroxybutyrate PHB which is a member ofpolyhydroxyalkanoate family have been widely used as a biomaterial for in vitro and in vivo studies due to its uniqueproperties such as improved flexibility and processability. In this study polyhydroxybutyrate scaffolds were preparedfor tissue engineering applications. In order to improve cell attachment on the scaffolds they were modified. Duringthe modification three different immunologically inactive compounds, polyetyhylene glycol PEG , 2-hydroxyethylmethacrylate HEMA and ethylenediamine EDA were used in radio frequency glow discharge RFGD plasmapolymerization system. Morphological evaluations were obtained by using scanning electron microscopy. Obtainedresults showed high and interconnected porosity. In vitro weight loss profiles of the scaffolds were investigated byusing gravimetric method and found to be influenced by PHB concentration used in the preparation of scaffolds. Theirbiological promotion of activities including cell attachment, morphology and proliferation on L929 mouse fibroblast cellswere examined and cytotoxicity tests were performed at the last part of the study

Keywords

Polyhydroxybutyrate,scaffold,tissue engineering,morphology,glow discharge

References

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Details

Primary Language

English

Subjects

-

Journal Section

-

Authors

Eylem Öztürk Güven This is me

Murat Demirbilek This is me

Necdet Sağlam This is me

Zeynep Karahaliloğlu This is me

Ebru Erdal This is me

Cem Bayram This is me

Emir Baki Denkbaş This is me

Publication Date

December 1, 2008

Submission Date

-

Acceptance Date

-

Published in Issue

Year 2008 Volume: 36 Number: 4

IZ

https://izlik.org/JA38EL72EB

Cite

RIS / Bibtex

APA

Öztürk Güven, E., Demirbilek, M., Sağlam, N., Karahaliloğlu, Z., Erdal, E., Bayram, C., & Denkbaş, E. B. (2008). Preparation and Characterization of Polyhydroxybutyrate Scaffolds to be Used in Tissue Engineering Applications. Hacettepe Journal of Biology and Chemistry, 36(4), 305-311. https://izlik.org/JA38EL72EB

AMA

1.Öztürk Güven E, Demirbilek M, Sağlam N, et al. Preparation and Characterization of Polyhydroxybutyrate Scaffolds to be Used in Tissue Engineering Applications. HJBC. 2008;36(4):305-311. https://izlik.org/JA38EL72EB

Chicago

Öztürk Güven, Eylem, Murat Demirbilek, Necdet Sağlam, et al. 2008. “Preparation and Characterization of Polyhydroxybutyrate Scaffolds to Be Used in Tissue Engineering Applications”. Hacettepe Journal of Biology and Chemistry 36 (4): 305-11. https://izlik.org/JA38EL72EB.

EndNote

Öztürk Güven E, Demirbilek M, Sağlam N, Karahaliloğlu Z, Erdal E, Bayram C, Denkbaş EB (December 1, 2008) Preparation and Characterization of Polyhydroxybutyrate Scaffolds to be Used in Tissue Engineering Applications. Hacettepe Journal of Biology and Chemistry 36 4 305–311.

IEEE

[1]E. Öztürk Güven et al., “Preparation and Characterization of Polyhydroxybutyrate Scaffolds to be Used in Tissue Engineering Applications”, HJBC, vol. 36, no. 4, pp. 305–311, Dec. 2008, [Online]. Available: https://izlik.org/JA38EL72EB

ISNAD

Öztürk Güven, Eylem - Demirbilek, Murat - Sağlam, Necdet - Karahaliloğlu, Zeynep - Erdal, Ebru - Bayram, Cem - Denkbaş, Emir Baki. “Preparation and Characterization of Polyhydroxybutyrate Scaffolds to Be Used in Tissue Engineering Applications”. Hacettepe Journal of Biology and Chemistry 36/4 (December 1, 2008): 305-311. https://izlik.org/JA38EL72EB.

JAMA

1.Öztürk Güven E, Demirbilek M, Sağlam N, Karahaliloğlu Z, Erdal E, Bayram C, Denkbaş EB. Preparation and Characterization of Polyhydroxybutyrate Scaffolds to be Used in Tissue Engineering Applications. HJBC. 2008;36:305–311.

MLA

Öztürk Güven, Eylem, et al. “Preparation and Characterization of Polyhydroxybutyrate Scaffolds to Be Used in Tissue Engineering Applications”. Hacettepe Journal of Biology and Chemistry, vol. 36, no. 4, Dec. 2008, pp. 305-11, https://izlik.org/JA38EL72EB.

Vancouver

1.Eylem Öztürk Güven, Murat Demirbilek, Necdet Sağlam, Zeynep Karahaliloğlu, Ebru Erdal, Cem Bayram, Emir Baki Denkbaş. Preparation and Characterization of Polyhydroxybutyrate Scaffolds to be Used in Tissue Engineering Applications. HJBC [Internet]. 2008 Dec. 1;36(4):305-11. Available from: https://izlik.org/JA38EL72EB